Confluentibacter flavum

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Confluentibacter

Average proteome isoelectric point is 6.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3459 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2N3HLW7|A0A2N3HLW7_9FLAO Cysteine synthase OS=Confluentibacter flavum OX=1909700 GN=cysK PE=3 SV=1
MM1 pKa = 7.24VPQIKK6 pKa = 9.91IFCNFIYY13 pKa = 9.99FIFILLSYY21 pKa = 10.73NAFAQSWSQSILTFSGTGNVNGGVTSLMYY50 pKa = 10.96GPDD53 pKa = 2.99NRR55 pKa = 11.84LYY57 pKa = 9.25VAEE60 pKa = 4.01YY61 pKa = 9.29TGAIKK66 pKa = 10.53ILTIQRR72 pKa = 11.84NSPINYY78 pKa = 8.16VATNVEE84 pKa = 4.03ILDD87 pKa = 5.04GIRR90 pKa = 11.84TMPDD94 pKa = 2.91HH95 pKa = 7.14NDD97 pKa = 3.28DD98 pKa = 3.64GTLYY102 pKa = 10.91SSLNRR107 pKa = 11.84EE108 pKa = 4.28TLGLTVVGTPTNPVIYY124 pKa = 9.21VTSSDD129 pKa = 3.48FRR131 pKa = 11.84IGAGEE136 pKa = 4.04GGGNGDD142 pKa = 4.1VNLDD146 pKa = 3.77TNSGVITRR154 pKa = 11.84FSWNGSAWNVVDD166 pKa = 4.41IVRR169 pKa = 11.84GLPRR173 pKa = 11.84SEE175 pKa = 4.39EE176 pKa = 3.77NHH178 pKa = 5.53ATNGLEE184 pKa = 3.74FVTINGTDD192 pKa = 3.73YY193 pKa = 11.43LIVAQGGHH201 pKa = 5.48TNGGSPSTNFVYY213 pKa = 10.41SCEE216 pKa = 4.12YY217 pKa = 10.32ALSGAILAVNLTALEE232 pKa = 4.18TMPILDD238 pKa = 4.46DD239 pKa = 3.59NGRR242 pKa = 11.84AYY244 pKa = 9.65IYY246 pKa = 9.82DD247 pKa = 4.85LPTLDD252 pKa = 5.59DD253 pKa = 3.58PTRR256 pKa = 11.84PNIHH260 pKa = 7.17ADD262 pKa = 3.57GSPASEE268 pKa = 5.5DD269 pKa = 3.69PDD271 pKa = 3.95DD272 pKa = 4.84PNYY275 pKa = 11.16SPLDD279 pKa = 3.56INDD282 pKa = 3.34PWGGNDD288 pKa = 4.45GLNQAILVPGGPVQILSPGYY308 pKa = 9.46RR309 pKa = 11.84NAYY312 pKa = 9.24DD313 pKa = 3.68LVVTEE318 pKa = 4.98SGALYY323 pKa = 9.26VTDD326 pKa = 3.85NGANGGWGGLPMNEE340 pKa = 4.28GGGSVTNDD348 pKa = 3.32YY349 pKa = 11.34NPAEE353 pKa = 4.71PGSSDD358 pKa = 3.18SLLYY362 pKa = 11.23GEE364 pKa = 4.89MVNNLDD370 pKa = 3.65HH371 pKa = 6.61LQLVTTNLQAYY382 pKa = 9.85AFGTLPEE389 pKa = 4.39TYY391 pKa = 10.22YY392 pKa = 10.95GGHH395 pKa = 7.19PNPTRR400 pKa = 11.84ANPVGAGLYY409 pKa = 8.79TADD412 pKa = 4.63GINNVFRR419 pKa = 11.84TLKK422 pKa = 10.08YY423 pKa = 10.78DD424 pKa = 3.98PDD426 pKa = 4.09GSTPGSTSNPNEE438 pKa = 4.17ALPVNWPPLPVSEE451 pKa = 4.66ANTVEE456 pKa = 4.25GDD458 pKa = 2.92WRR460 pKa = 11.84GPGMINPDD468 pKa = 4.11GPNDD472 pKa = 3.87DD473 pKa = 5.15PVVTWGTNTNGIDD486 pKa = 3.68EE487 pKa = 4.46YY488 pKa = 10.41TASNFGGVMQGNLLAGTHH506 pKa = 6.22NGLIRR511 pKa = 11.84RR512 pKa = 11.84VQLNANGTLASGGLTSNFLSGMGGNALGITCNSDD546 pKa = 3.05FDD548 pKa = 4.22IFPGTVWAGTLNGKK562 pKa = 9.23IVVFEE567 pKa = 3.96PTDD570 pKa = 3.61FLNCLDD576 pKa = 4.85PSDD579 pKa = 4.66PLYY582 pKa = 10.97DD583 pKa = 3.82ANADD587 pKa = 3.79YY588 pKa = 11.32DD589 pKa = 3.92SDD591 pKa = 4.47GYY593 pKa = 11.5SNQDD597 pKa = 3.17EE598 pKa = 4.55EE599 pKa = 5.8DD600 pKa = 3.8NGTEE604 pKa = 3.67ICNGGSQPDD613 pKa = 3.6DD614 pKa = 3.4WDD616 pKa = 3.53KK617 pKa = 12.1VMGGQLISDD626 pKa = 4.74LNDD629 pKa = 4.12PDD631 pKa = 5.81DD632 pKa = 5.53DD633 pKa = 4.91FDD635 pKa = 5.96GIPDD639 pKa = 4.01ALDD642 pKa = 3.78PFQLGDD648 pKa = 3.64PTVEE652 pKa = 4.03GSDD655 pKa = 3.35AFTIPILNDD664 pKa = 3.51FFNDD668 pKa = 3.48QQNLGGIYY676 pKa = 10.53GLGLTGLMNNGDD688 pKa = 3.8TGANWLLWLDD698 pKa = 4.53RR699 pKa = 11.84KK700 pKa = 10.6DD701 pKa = 5.34DD702 pKa = 4.61PNDD705 pKa = 3.89PNPNDD710 pKa = 3.76ILGGAPGLMTSQMTSGTALGTSNNQEE736 pKa = 3.44KK737 pKa = 10.32GYY739 pKa = 10.56QYY741 pKa = 11.22GVQIQNDD748 pKa = 3.62SGIITVSGGMVGFNGPLTLYY768 pKa = 9.82EE769 pKa = 4.29NLSAPSGEE777 pKa = 4.04LGFFIGDD784 pKa = 3.93GTQSNYY790 pKa = 10.1IKK792 pKa = 10.61FVVTTTGFVALQEE805 pKa = 4.52IEE807 pKa = 5.82DD808 pKa = 4.28IPQSPITYY816 pKa = 9.82NLPQANRR823 pKa = 11.84PTSGIKK829 pKa = 10.12FFFEE833 pKa = 4.04IDD835 pKa = 3.48PSIGQVTLKK844 pKa = 10.51FQIDD848 pKa = 3.88EE849 pKa = 4.6GSLTTLGTIVAQGSILQALQSSSTDD874 pKa = 3.46LAVGLIGASNEE885 pKa = 4.12DD886 pKa = 3.65GVEE889 pKa = 5.15LEE891 pKa = 4.44GTWDD895 pKa = 3.77FLNVTAEE902 pKa = 4.1KK903 pKa = 10.25PYY905 pKa = 9.99MVQNIPNLQRR915 pKa = 11.84EE916 pKa = 4.43SGAPDD921 pKa = 4.11DD922 pKa = 5.41MFDD925 pKa = 4.38LDD927 pKa = 6.57DD928 pKa = 4.36YY929 pKa = 11.91FEE931 pKa = 5.82DD932 pKa = 4.24NNGTANLTYY941 pKa = 10.67SVALNTNPNILASISNNLLTITYY964 pKa = 9.31PSEE967 pKa = 3.89LQTADD972 pKa = 2.82ITIRR976 pKa = 11.84ASDD979 pKa = 3.61LNGYY983 pKa = 8.27FVEE986 pKa = 4.55DD987 pKa = 3.93TFTVSIINSSLILARR1002 pKa = 11.84VNAGGAAVSATDD1014 pKa = 5.01GGANWEE1020 pKa = 4.24QNNVAGATSGPSYY1033 pKa = 10.95SVNTGTIYY1041 pKa = 10.96NGGLLNINRR1050 pKa = 11.84HH1051 pKa = 4.87SSIPDD1056 pKa = 3.62YY1057 pKa = 10.86IDD1059 pKa = 3.22EE1060 pKa = 4.32STYY1063 pKa = 11.16NAIFAYY1069 pKa = 9.71EE1070 pKa = 4.25RR1071 pKa = 11.84YY1072 pKa = 9.89DD1073 pKa = 3.43VTASPEE1079 pKa = 3.9MEE1081 pKa = 4.09FTFVLQNGDD1090 pKa = 3.83YY1091 pKa = 10.37QVNLYY1096 pKa = 8.86MGNSFVGTSEE1106 pKa = 3.41IGDD1109 pKa = 3.47RR1110 pKa = 11.84VFDD1113 pKa = 3.73IEE1115 pKa = 4.18IEE1117 pKa = 4.28GVIVEE1122 pKa = 4.97DD1123 pKa = 4.58NMDD1126 pKa = 4.84LIARR1130 pKa = 11.84FGHH1133 pKa = 5.6QSGGMVTYY1141 pKa = 9.79PVTVSGGVLNIQFLHH1156 pKa = 5.79EE1157 pKa = 4.36VEE1159 pKa = 4.75NPLLNAIEE1167 pKa = 4.36IMAAPPQYY1175 pKa = 10.27PPLSVNPIANQTNLTGDD1192 pKa = 3.86VISTLGVSASGGNPNNNFTYY1212 pKa = 10.55SIDD1215 pKa = 3.8GQPLGINIEE1224 pKa = 4.16PTNGLIYY1231 pKa = 9.34GTIDD1235 pKa = 3.11QTASNGGTNNDD1246 pKa = 3.28GVHH1249 pKa = 5.87GVTVTVEE1256 pKa = 4.43KK1257 pKa = 10.91VGSTSVTTNFSWTVNDD1273 pKa = 5.07LAWFDD1278 pKa = 3.87KK1279 pKa = 11.33NEE1281 pKa = 4.03NEE1283 pKa = 4.5NYY1285 pKa = 7.42TARR1288 pKa = 11.84HH1289 pKa = 5.42EE1290 pKa = 4.43CSFVQAGDD1298 pKa = 3.21KK1299 pKa = 10.58FYY1301 pKa = 11.44LLGGRR1306 pKa = 11.84EE1307 pKa = 3.93NSKK1310 pKa = 8.31TLDD1313 pKa = 3.41VYY1315 pKa = 11.5NYY1317 pKa = 9.34TSNSWNSLVDD1327 pKa = 3.78SAPMEE1332 pKa = 4.47FNHH1335 pKa = 6.4FQATEE1340 pKa = 3.94YY1341 pKa = 10.59QGLIWVIGAFQTNSFPNEE1359 pKa = 3.82TPADD1363 pKa = 4.47FIWAFDD1369 pKa = 3.69PVAQQWIQGPEE1380 pKa = 3.73IPSGRR1385 pKa = 11.84RR1386 pKa = 11.84RR1387 pKa = 11.84GSAGLVVHH1395 pKa = 6.63NDD1397 pKa = 2.33KK1398 pKa = 11.02FYY1400 pKa = 10.56IVGGNTIGHH1409 pKa = 6.3NGGYY1413 pKa = 9.77VPWFDD1418 pKa = 4.53EE1419 pKa = 4.25YY1420 pKa = 11.74DD1421 pKa = 3.68PVLGTWTTLTNAPRR1435 pKa = 11.84SRR1437 pKa = 11.84DD1438 pKa = 3.32HH1439 pKa = 5.98FHH1441 pKa = 7.21AVVINNKK1448 pKa = 9.77LYY1450 pKa = 10.3AASGRR1455 pKa = 11.84LSGGDD1460 pKa = 3.13GGTFAPVIPEE1470 pKa = 3.52VDD1472 pKa = 3.49VYY1474 pKa = 11.66DD1475 pKa = 4.8FSTNSWSSLPSDD1487 pKa = 3.72QNIPTPRR1494 pKa = 11.84AGAIVANFNNKK1505 pKa = 8.84LVIAGGEE1512 pKa = 3.96VSTNVNALDD1521 pKa = 3.14ITEE1524 pKa = 4.94IYY1526 pKa = 10.52DD1527 pKa = 4.14PLDD1530 pKa = 3.57QSWSTGASLIHH1541 pKa = 6.57PRR1543 pKa = 11.84HH1544 pKa = 5.0GTQGIVSGNGIFVVAGSPVMGGGKK1568 pKa = 8.8QKK1570 pKa = 10.94NMEE1573 pKa = 4.52FYY1575 pKa = 10.98GSDD1578 pKa = 3.64SPTGIPLDD1586 pKa = 3.76ASEE1589 pKa = 5.27LMGPTTVDD1597 pKa = 3.7FSVQNSQDD1605 pKa = 2.85ITLDD1609 pKa = 3.67LEE1611 pKa = 4.49NGNTGIFIRR1620 pKa = 11.84SVEE1623 pKa = 4.24INGHH1627 pKa = 6.6DD1628 pKa = 4.32DD1629 pKa = 3.74DD1630 pKa = 6.52DD1631 pKa = 4.98FVIQSGNLNNVLLKK1645 pKa = 10.9SNSSKK1650 pKa = 10.51IISVDD1655 pKa = 4.11FIGAGTEE1662 pKa = 3.88KK1663 pKa = 10.82NAILTIDD1670 pKa = 3.52YY1671 pKa = 8.84GANASLDD1678 pKa = 3.15ISLSYY1683 pKa = 11.19DD1684 pKa = 3.46STLSSPDD1691 pKa = 3.17EE1692 pKa = 4.34KK1693 pKa = 11.61DD1694 pKa = 3.34NISNVFTIYY1703 pKa = 10.36PNPSSEE1709 pKa = 4.05YY1710 pKa = 8.91VYY1712 pKa = 11.31VEE1714 pKa = 4.34IKK1716 pKa = 10.56SSSIDD1721 pKa = 3.27VTDD1724 pKa = 4.65FLLFDD1729 pKa = 3.15ITGRR1733 pKa = 11.84LIKK1736 pKa = 10.15KK1737 pKa = 9.48IKK1739 pKa = 10.04NDD1741 pKa = 3.16NSEE1744 pKa = 3.51QSTFKK1749 pKa = 10.83KK1750 pKa = 10.58QIDD1753 pKa = 3.69VRR1755 pKa = 11.84GLQSGLYY1762 pKa = 8.59TLYY1765 pKa = 10.17ILSKK1769 pKa = 10.52GSVVHH1774 pKa = 6.88KK1775 pKa = 10.7ARR1777 pKa = 11.84LVINNN1782 pKa = 3.78

Molecular weight: 190.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2N3HN48|A0A2N3HN48_9FLAO Uncharacterized protein OS=Confluentibacter flavum OX=1909700 GN=CSW08_04105 PE=4 SV=1
MM1 pKa = 7.69SKK3 pKa = 9.01RR4 pKa = 11.84TFQPSKK10 pKa = 9.13RR11 pKa = 11.84KK12 pKa = 9.48RR13 pKa = 11.84RR14 pKa = 11.84NKK16 pKa = 9.49HH17 pKa = 3.94GFRR20 pKa = 11.84EE21 pKa = 4.27RR22 pKa = 11.84MASANGRR29 pKa = 11.84KK30 pKa = 9.04VLARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84AKK38 pKa = 10.09GRR40 pKa = 11.84KK41 pKa = 7.97KK42 pKa = 10.6LSVSSEE48 pKa = 4.04SRR50 pKa = 11.84HH51 pKa = 5.63KK52 pKa = 10.69KK53 pKa = 9.55

Molecular weight: 6.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3459

0

3459

1212577

38

2585

350.6

39.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.072 ± 0.037

0.772 ± 0.013

5.725 ± 0.033

6.317 ± 0.037

5.277 ± 0.032

6.419 ± 0.043

1.86 ± 0.022

8.324 ± 0.043

7.737 ± 0.048

9.27 ± 0.04

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.239 ± 0.021

6.499 ± 0.047

3.417 ± 0.023

3.196 ± 0.025

3.257 ± 0.025

6.528 ± 0.03

5.79 ± 0.034

6.061 ± 0.031

1.12 ± 0.018

4.12 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski