Ralstonia solanacearum (strain GMI1000) (Pseudomonas solanacearum)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Burkholderiaceae; Ralstonia; Ralstonia solanacearum

Average proteome isoelectric point is 6.97

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5002 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q8XSB9|Q8XSB9_RALSO Putative transmembrane protein OS=Ralstonia solanacearum (strain GMI1000) OX=267608 GN=RSp0559 PE=4 SV=1
MM1 pKa = 8.26DD2 pKa = 5.07IPTANYY8 pKa = 9.5NAFVTEE14 pKa = 4.48LTAITCKK21 pKa = 10.79YY22 pKa = 9.91GVALTAIGGVSIADD36 pKa = 3.49EE37 pKa = 4.51PGAFRR42 pKa = 11.84DD43 pKa = 3.66VAYY46 pKa = 10.8VADD49 pKa = 3.81ITSGDD54 pKa = 4.36LYY56 pKa = 11.44AKK58 pKa = 10.55DD59 pKa = 3.95PEE61 pKa = 4.47SS62 pKa = 3.32

Molecular weight: 6.47 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q8XZC0|Q8XZC0_RALSO Probable transmembrane protein OS=Ralstonia solanacearum (strain GMI1000) OX=267608 GN=RSc1481 PE=4 SV=1
MM1 pKa = 8.7VLMLPQARR9 pKa = 11.84SGAQVRR15 pKa = 11.84AVRR18 pKa = 11.84VSAMSRR24 pKa = 11.84PVASAVRR31 pKa = 11.84VPVAKK36 pKa = 10.58VAGKK40 pKa = 8.97PVARR44 pKa = 11.84AKK46 pKa = 10.22PVASASAKK54 pKa = 8.93RR55 pKa = 11.84RR56 pKa = 11.84KK57 pKa = 9.41RR58 pKa = 3.4

Molecular weight: 6.08 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5002

0

5002

1662664

20

6889

332.4

35.96

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.414 ± 0.055

0.936 ± 0.013

5.403 ± 0.029

4.898 ± 0.037

3.368 ± 0.026

8.406 ± 0.051

2.323 ± 0.024

4.357 ± 0.025

2.843 ± 0.033

10.305 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.332 ± 0.019

2.689 ± 0.041

5.392 ± 0.034

3.896 ± 0.023

7.284 ± 0.051

5.318 ± 0.042

5.564 ± 0.059

7.499 ± 0.032

1.413 ± 0.015

2.361 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski