Eubacterium saphenum ATCC 49989

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Eubacteriales incertae sedis; Eubacteriales Family XIII. Incertae Sedis; [Eubacterium] saphenum

Average proteome isoelectric point is 6.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 952 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C7H0V8|C7H0V8_9FIRM Bacterial transferase hexapeptide repeat protein OS=Eubacterium saphenum ATCC 49989 OX=592031 GN=GCWU000322_00883 PE=4 SV=1
MM1 pKa = 7.43AKK3 pKa = 9.99VAVVYY8 pKa = 9.79WSGTGNTEE16 pKa = 3.61YY17 pKa = 9.73MANSIVEE24 pKa = 4.24GAKK27 pKa = 10.16GAGAEE32 pKa = 4.3VEE34 pKa = 4.45TFTAGDD40 pKa = 3.86FSPAKK45 pKa = 9.97VAEE48 pKa = 4.04FDD50 pKa = 4.22VIAFGCPSMGEE61 pKa = 4.1EE62 pKa = 3.99VLEE65 pKa = 4.24EE66 pKa = 4.51SEE68 pKa = 4.72FAPMWDD74 pKa = 3.38EE75 pKa = 4.42VKK77 pKa = 11.18GEE79 pKa = 3.96LSGKK83 pKa = 9.63KK84 pKa = 9.08VALFGSYY91 pKa = 9.06GWGDD95 pKa = 4.14GEE97 pKa = 4.83WMRR100 pKa = 11.84NWVAEE105 pKa = 4.18CNEE108 pKa = 3.95IGATLIGEE116 pKa = 4.14GLMINEE122 pKa = 4.38TPDD125 pKa = 3.51DD126 pKa = 4.28EE127 pKa = 5.42GVEE130 pKa = 4.21EE131 pKa = 4.8CKK133 pKa = 10.9ALGASLAA140 pKa = 3.9

Molecular weight: 14.85 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C7H0Y6|C7H0Y6_9FIRM 30S ribosomal protein S5 OS=Eubacterium saphenum ATCC 49989 OX=592031 GN=rpsE PE=3 SV=1
MM1 pKa = 7.83AKK3 pKa = 9.58TSLKK7 pKa = 10.36VKK9 pKa = 9.73QKK11 pKa = 10.44RR12 pKa = 11.84KK13 pKa = 9.31PKK15 pKa = 9.96FSTRR19 pKa = 11.84AYY21 pKa = 8.11TRR23 pKa = 11.84CRR25 pKa = 11.84VCGRR29 pKa = 11.84PHH31 pKa = 7.1SVLKK35 pKa = 10.64KK36 pKa = 10.27YY37 pKa = 9.54GICRR41 pKa = 11.84ICFRR45 pKa = 11.84EE46 pKa = 3.81LAYY49 pKa = 10.0KK50 pKa = 10.91GEE52 pKa = 4.05IPGVKK57 pKa = 9.3KK58 pKa = 10.94ASWW61 pKa = 3.03

Molecular weight: 7.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

952

0

952

328471

39

4743

345.0

38.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.557 ± 0.086

1.125 ± 0.035

6.155 ± 0.062

7.115 ± 0.106

4.132 ± 0.055

7.165 ± 0.073

1.525 ± 0.033

8.201 ± 0.113

9.39 ± 0.118

8.395 ± 0.116

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.855 ± 0.042

4.842 ± 0.091

3.006 ± 0.042

2.151 ± 0.045

4.175 ± 0.067

6.239 ± 0.068

4.865 ± 0.077

6.89 ± 0.063

0.663 ± 0.035

3.553 ± 0.046
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski