TTV-like mini virus

Taxonomy: Viruses; Anelloviridae; Betatorquevirus; unclassified Betatorquevirus

Average proteome isoelectric point is 7.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|V5NT04|V5NT04_9VIRU Uncharacterized protein OS=TTV-like mini virus OX=93678 GN=ORF3 PE=4 SV=1
MM1 pKa = 7.62SMPFSTKK8 pKa = 9.04KK9 pKa = 10.28QRR11 pKa = 11.84LQLCNLVTGIHH22 pKa = 7.6DD23 pKa = 3.72ITCNCYY29 pKa = 10.37NPLFHH34 pKa = 7.14SAQIILKK41 pKa = 9.83QLAPEE46 pKa = 4.2LKK48 pKa = 10.27KK49 pKa = 10.86EE50 pKa = 4.29EE51 pKa = 3.96KK52 pKa = 10.22HH53 pKa = 6.25QLKK56 pKa = 9.85QCLTEE61 pKa = 3.87EE62 pKa = 4.39TTTKK66 pKa = 10.68EE67 pKa = 3.96EE68 pKa = 4.07EE69 pKa = 4.37DD70 pKa = 3.55VGFTTGDD77 pKa = 3.46LEE79 pKa = 5.31ALFAEE84 pKa = 4.82NGDD87 pKa = 3.96DD88 pKa = 4.11TEE90 pKa = 4.61DD91 pKa = 3.47AAGG94 pKa = 3.49

Molecular weight: 10.54 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|V5NSF8|V5NSF8_9VIRU Uncharacterized protein OS=TTV-like mini virus OX=93678 GN=ORF2 PE=4 SV=1
MM1 pKa = 8.07PYY3 pKa = 10.06RR4 pKa = 11.84RR5 pKa = 11.84NYY7 pKa = 6.8YY8 pKa = 7.33QRR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84RR13 pKa = 11.84RR14 pKa = 11.84WIYY17 pKa = 8.6YY18 pKa = 8.77RR19 pKa = 11.84RR20 pKa = 11.84PRR22 pKa = 11.84GPFRR26 pKa = 11.84RR27 pKa = 11.84KK28 pKa = 5.31WRR30 pKa = 11.84RR31 pKa = 11.84YY32 pKa = 7.83RR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84WVRR39 pKa = 11.84KK40 pKa = 9.13KK41 pKa = 10.29KK42 pKa = 10.85LPFLRR47 pKa = 11.84LKK49 pKa = 10.26EE50 pKa = 4.07YY51 pKa = 10.53QPSNIRR57 pKa = 11.84KK58 pKa = 9.41CKK60 pKa = 9.04IQGLIPLYY68 pKa = 9.67WGTPEE73 pKa = 5.06RR74 pKa = 11.84FVNNYY79 pKa = 10.12DD80 pKa = 3.62SYY82 pKa = 11.92EE83 pKa = 4.11MTTAPPKK90 pKa = 10.47LPSGGLFTIKK100 pKa = 10.45NFSLQSLYY108 pKa = 11.14SEE110 pKa = 3.8NRR112 pKa = 11.84YY113 pKa = 8.87IRR115 pKa = 11.84NTWSHH120 pKa = 6.23TNTDD124 pKa = 4.02LPLVRR129 pKa = 11.84YY130 pKa = 6.99TGCKK134 pKa = 8.64FKK136 pKa = 10.3IYY138 pKa = 10.12KK139 pKa = 9.63SEE141 pKa = 4.07HH142 pKa = 6.02VDD144 pKa = 3.92TILTFDD150 pKa = 4.02NNLPLASNQDD160 pKa = 3.98MYY162 pKa = 11.92LSMQPSVHH170 pKa = 7.67SMIKK174 pKa = 9.97HH175 pKa = 6.14KK176 pKa = 10.52IIIPRR181 pKa = 11.84LTKK184 pKa = 10.42GYY186 pKa = 10.21NKK188 pKa = 10.12KK189 pKa = 9.97PYY191 pKa = 8.45KK192 pKa = 9.31TIHH195 pKa = 6.27IKK197 pKa = 10.08PPKK200 pKa = 9.66PMINKK205 pKa = 9.03WYY207 pKa = 8.65FQQDD211 pKa = 2.66ITTIPLCQIRR221 pKa = 11.84ASACSLNEE229 pKa = 3.24WYY231 pKa = 9.91INYY234 pKa = 9.8KK235 pKa = 10.29SVSTTSTINFLNPGCITNTYY255 pKa = 8.43FNKK258 pKa = 10.27YY259 pKa = 10.1YY260 pKa = 9.53STGWYY265 pKa = 9.36ARR267 pKa = 11.84KK268 pKa = 9.56LDD270 pKa = 4.21SGTKK274 pKa = 9.44IYY276 pKa = 10.72LISFKK281 pKa = 11.02NNEE284 pKa = 3.48QHH286 pKa = 4.99TWEE289 pKa = 4.12NTVFLGNTLDD299 pKa = 3.76NQEE302 pKa = 3.98GKK304 pKa = 8.1TPKK307 pKa = 10.27EE308 pKa = 3.77INLKK312 pKa = 10.53SKK314 pKa = 10.86GSTTQDD320 pKa = 2.09IMQXYY325 pKa = 9.13GKK327 pKa = 8.94QQWGNPFYY335 pKa = 11.23KK336 pKa = 10.46NYY338 pKa = 10.72LNNTWKK344 pKa = 10.66VYY346 pKa = 9.68FFNEE350 pKa = 3.86EE351 pKa = 4.13LFTFLDD357 pKa = 3.97QYY359 pKa = 10.34RR360 pKa = 11.84QHH362 pKa = 7.38PNTPIPVTYY371 pKa = 8.99ITEE374 pKa = 4.28TQLTNAIRR382 pKa = 11.84YY383 pKa = 8.17NPLSDD388 pKa = 3.4EE389 pKa = 4.54GKK391 pKa = 8.76DD392 pKa = 3.08TKK394 pKa = 10.71IYY396 pKa = 9.85FKK398 pKa = 9.8PTNQPNEE405 pKa = 4.26DD406 pKa = 3.65WDD408 pKa = 4.17PPQNPNLMAVNLPLWILTFGFVDD431 pKa = 3.17YY432 pKa = 10.68HH433 pKa = 7.14KK434 pKa = 10.82KK435 pKa = 10.37LKK437 pKa = 9.19QVKK440 pKa = 10.31NIDD443 pKa = 3.45TEE445 pKa = 4.32QILTIQAKK453 pKa = 10.35LDD455 pKa = 3.61GPITKK460 pKa = 9.56VYY462 pKa = 10.18PLIDD466 pKa = 3.39SDD468 pKa = 4.73FIAGKK473 pKa = 9.96SPYY476 pKa = 9.5EE477 pKa = 4.07QVPDD481 pKa = 4.01PLDD484 pKa = 3.44YY485 pKa = 11.34NRR487 pKa = 11.84WHH489 pKa = 7.26LSFQFQQITTNNIGLSGPGSVKK511 pKa = 10.62SPTLQAVQAKK521 pKa = 9.01CHH523 pKa = 4.15YY524 pKa = 9.17TFYY527 pKa = 10.75FKK529 pKa = 10.24WGGNPPPMQPITDD542 pKa = 3.82PTKK545 pKa = 9.62QPHH548 pKa = 6.09YY549 pKa = 9.77NLPFNITKK557 pKa = 7.51TTSLQNPATSPAYY570 pKa = 10.17SLYY573 pKa = 10.06TFDD576 pKa = 3.69YY577 pKa = 10.74RR578 pKa = 11.84RR579 pKa = 11.84GXLTTKK585 pKa = 10.54AIEE588 pKa = 4.13RR589 pKa = 11.84LQKK592 pKa = 10.6DD593 pKa = 4.0FPLKK597 pKa = 9.81KK598 pKa = 9.27TFITDD603 pKa = 3.32SEE605 pKa = 4.63RR606 pKa = 11.84FQPPLQTQQEE616 pKa = 4.63TTSEE620 pKa = 4.15SSSEE624 pKa = 4.04EE625 pKa = 3.88EE626 pKa = 3.99EE627 pKa = 4.55EE628 pKa = 4.99EE629 pKa = 5.3ISTLLNKK636 pKa = 10.23LQRR639 pKa = 11.84QRR641 pKa = 11.84HH642 pKa = 4.53KK643 pKa = 10.83QKK645 pKa = 10.1QLKK648 pKa = 9.75YY649 pKa = 10.61LILKK653 pKa = 9.24NLGYY657 pKa = 10.35LPKK660 pKa = 10.71LEE662 pKa = 4.13

Molecular weight: 78.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

882

94

662

294.0

34.5

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

2.608 ± 1.119

1.134 ± 0.913

3.968 ± 0.704

5.669 ± 2.271

4.422 ± 0.164

3.741 ± 0.721

1.927 ± 0.367

6.122 ± 0.793

8.844 ± 0.212

9.524 ± 1.336

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.361 ± 0.252

6.463 ± 1.158

7.37 ± 1.222

6.689 ± 0.877

4.989 ± 1.202

5.669 ± 0.768

9.524 ± 0.902

2.041 ± 0.639

1.701 ± 0.834

5.896 ± 1.587
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski