Amino acid dipepetide frequency for Japanese holly fern mottle virus

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
5.155AlaAla: 5.155 ± 1.627
1.375AlaCys: 1.375 ± 0.787
5.498AlaAsp: 5.498 ± 1.742
3.436AlaGlu: 3.436 ± 0.667
3.093AlaPhe: 3.093 ± 1.85
3.436AlaGly: 3.436 ± 2.138
0.344AlaHis: 0.344 ± 0.192
2.062AlaIle: 2.062 ± 0.832
2.405AlaLys: 2.405 ± 0.789
5.498AlaLeu: 5.498 ± 1.313
2.405AlaMet: 2.405 ± 0.935
1.375AlaAsn: 1.375 ± 0.595
2.062AlaPro: 2.062 ± 0.832
2.062AlaGln: 2.062 ± 0.726
2.062AlaArg: 2.062 ± 1.508
7.216AlaSer: 7.216 ± 0.946
2.405AlaThr: 2.405 ± 0.986
11.34AlaVal: 11.34 ± 3.261
0.687AlaTrp: 0.687 ± 0.384
1.718AlaTyr: 1.718 ± 0.631
0.0AlaXaa: 0.0 ± 0.0
Cys
2.062CysAla: 2.062 ± 0.788
0.687CysCys: 0.687 ± 0.384
2.749CysAsp: 2.749 ± 1.054
0.344CysGlu: 0.344 ± 0.576
0.344CysPhe: 0.344 ± 0.192
1.031CysGly: 1.031 ± 0.467
1.718CysHis: 1.718 ± 0.96
0.0CysIle: 0.0 ± 0.0
1.718CysLys: 1.718 ± 0.701
2.062CysLeu: 2.062 ± 0.933
1.375CysMet: 1.375 ± 0.595
0.687CysAsn: 0.687 ± 0.384
1.375CysPro: 1.375 ± 0.521
1.031CysGln: 1.031 ± 0.544
1.031CysArg: 1.031 ± 0.626
2.405CysSer: 2.405 ± 0.92
0.0CysThr: 0.0 ± 0.0
3.436CysVal: 3.436 ± 1.08
0.687CysTrp: 0.687 ± 1.111
1.031CysTyr: 1.031 ± 0.467
0.0CysXaa: 0.0 ± 0.0
Asp
4.124AspAla: 4.124 ± 1.186
0.687AspCys: 0.687 ± 0.384
5.155AspAsp: 5.155 ± 0.705
4.811AspGlu: 4.811 ± 1.078
1.375AspPhe: 1.375 ± 0.768
3.436AspGly: 3.436 ± 1.79
1.375AspHis: 1.375 ± 0.576
4.811AspIle: 4.811 ± 1.71
4.124AspLys: 4.124 ± 1.864
7.56AspLeu: 7.56 ± 1.521
1.718AspMet: 1.718 ± 0.701
1.375AspAsn: 1.375 ± 0.768
4.124AspPro: 4.124 ± 1.32
1.031AspGln: 1.031 ± 0.45
4.467AspArg: 4.467 ± 1.023
4.811AspSer: 4.811 ± 1.564
2.062AspThr: 2.062 ± 1.152
8.247AspVal: 8.247 ± 1.565
0.344AspTrp: 0.344 ± 0.192
1.375AspTyr: 1.375 ± 0.768
0.0AspXaa: 0.0 ± 0.0
Glu
3.78GluAla: 3.78 ± 1.078
1.031GluCys: 1.031 ± 0.576
1.718GluAsp: 1.718 ± 0.631
3.093GluGlu: 3.093 ± 2.078
2.062GluPhe: 2.062 ± 0.774
2.749GluGly: 2.749 ± 1.174
1.031GluHis: 1.031 ± 1.009
2.405GluIle: 2.405 ± 0.935
3.436GluLys: 3.436 ± 2.328
5.155GluLeu: 5.155 ± 1.879
1.031GluMet: 1.031 ± 0.474
1.375GluAsn: 1.375 ± 0.521
5.498GluPro: 5.498 ± 3.43
1.375GluGln: 1.375 ± 0.768
2.062GluArg: 2.062 ± 0.774
3.78GluSer: 3.78 ± 0.525
1.375GluThr: 1.375 ± 0.521
4.467GluVal: 4.467 ± 1.036
0.687GluTrp: 0.687 ± 0.384
3.093GluTyr: 3.093 ± 1.324
0.0GluXaa: 0.0 ± 0.0
Phe
2.749PheAla: 2.749 ± 1.06
2.062PheCys: 2.062 ± 0.774
5.498PheAsp: 5.498 ± 2.05
5.842PheGlu: 5.842 ± 2.228
1.031PhePhe: 1.031 ± 0.576
1.375PheGly: 1.375 ± 0.787
1.375PheHis: 1.375 ± 0.984
2.749PheIle: 2.749 ± 1.192
2.062PheLys: 2.062 ± 0.832
3.093PheLeu: 3.093 ± 1.324
0.344PheMet: 0.344 ± 0.632
1.718PheAsn: 1.718 ± 0.96
2.405PhePro: 2.405 ± 0.525
1.375PheGln: 1.375 ± 0.521
3.78PheArg: 3.78 ± 1.128
6.873PheSer: 6.873 ± 1.994
2.749PheThr: 2.749 ± 0.696
3.78PheVal: 3.78 ± 1.475
0.344PheTrp: 0.344 ± 0.576
1.718PheTyr: 1.718 ± 0.587
0.0PheXaa: 0.0 ± 0.0
Gly
4.467GlyAla: 4.467 ± 0.598
1.031GlyCys: 1.031 ± 0.576
6.186GlyAsp: 6.186 ± 1.626
2.405GlyGlu: 2.405 ± 0.873
5.498GlyPhe: 5.498 ± 0.869
2.405GlyGly: 2.405 ± 0.963
0.687GlyHis: 0.687 ± 0.492
1.718GlyIle: 1.718 ± 1.395
3.78GlyLys: 3.78 ± 1.487
2.749GlyLeu: 2.749 ± 1.775
1.718GlyMet: 1.718 ± 0.558
1.718GlyAsn: 1.718 ± 0.924
1.718GlyPro: 1.718 ± 0.714
1.031GlyGln: 1.031 ± 0.717
2.405GlyArg: 2.405 ± 1.014
3.78GlySer: 3.78 ± 0.525
2.405GlyThr: 2.405 ± 1.297
5.155GlyVal: 5.155 ± 0.667
1.031GlyTrp: 1.031 ± 0.906
1.031GlyTyr: 1.031 ± 0.717
0.0GlyXaa: 0.0 ± 0.0
His
1.031HisAla: 1.031 ± 0.691
0.344HisCys: 0.344 ± 0.192
1.718HisAsp: 1.718 ± 0.631
0.0HisGlu: 0.0 ± 0.0
2.062HisPhe: 2.062 ± 0.474
2.062HisGly: 2.062 ± 0.99
0.687HisHis: 0.687 ± 0.492
0.687HisIle: 0.687 ± 0.384
1.031HisLys: 1.031 ± 1.076
3.093HisLeu: 3.093 ± 3.587
1.031HisMet: 1.031 ± 0.576
0.687HisAsn: 0.687 ± 0.488
0.687HisPro: 0.687 ± 0.488
0.344HisGln: 0.344 ± 0.192
1.718HisArg: 1.718 ± 0.701
1.718HisSer: 1.718 ± 0.96
0.687HisThr: 0.687 ± 0.384
2.749HisVal: 2.749 ± 1.406
0.0HisTrp: 0.0 ± 0.0
1.031HisTyr: 1.031 ± 1.009
0.0HisXaa: 0.0 ± 0.0
Ile
1.031IleAla: 1.031 ± 0.45
0.687IleCys: 0.687 ± 0.384
3.78IleAsp: 3.78 ± 0.958
2.062IleGlu: 2.062 ± 1.152
1.031IlePhe: 1.031 ± 0.576
2.405IleGly: 2.405 ± 1.124
0.687IleHis: 0.687 ± 0.384
1.031IleIle: 1.031 ± 0.45
1.031IleLys: 1.031 ± 0.45
2.405IleLeu: 2.405 ± 1.344
0.687IleMet: 0.687 ± 0.558
1.375IleAsn: 1.375 ± 0.595
4.124IlePro: 4.124 ± 1.562
0.687IleGln: 0.687 ± 0.384
2.749IleArg: 2.749 ± 1.19
5.498IleSer: 5.498 ± 2.714
2.405IleThr: 2.405 ± 0.986
3.093IleVal: 3.093 ± 0.251
0.344IleTrp: 0.344 ± 0.576
1.031IleTyr: 1.031 ± 0.467
0.0IleXaa: 0.0 ± 0.0
Lys
3.093LysAla: 3.093 ± 1.671
0.687LysCys: 0.687 ± 0.488
4.124LysAsp: 4.124 ± 1.176
4.467LysGlu: 4.467 ± 1.226
3.78LysPhe: 3.78 ± 1.172
3.78LysGly: 3.78 ± 0.531
1.031LysHis: 1.031 ± 1.368
2.749LysIle: 2.749 ± 0.696
2.749LysLys: 2.749 ± 1.535
4.124LysLeu: 4.124 ± 1.668
1.031LysMet: 1.031 ± 0.544
1.375LysAsn: 1.375 ± 0.595
3.436LysPro: 3.436 ± 1.77
2.062LysGln: 2.062 ± 0.542
2.062LysArg: 2.062 ± 0.51
5.498LysSer: 5.498 ± 1.776
2.749LysThr: 2.749 ± 0.986
3.093LysVal: 3.093 ± 1.631
1.031LysTrp: 1.031 ± 0.576
2.062LysTyr: 2.062 ± 0.773
0.0LysXaa: 0.0 ± 0.0
Leu
5.155LeuAla: 5.155 ± 0.87
2.749LeuCys: 2.749 ± 0.696
2.749LeuAsp: 2.749 ± 0.633
5.842LeuGlu: 5.842 ± 0.824
4.467LeuPhe: 4.467 ± 1.878
4.124LeuGly: 4.124 ± 1.207
1.375LeuHis: 1.375 ± 1.617
3.436LeuIle: 3.436 ± 0.996
5.155LeuLys: 5.155 ± 0.718
5.155LeuLeu: 5.155 ± 1.273
1.375LeuMet: 1.375 ± 0.768
4.124LeuAsn: 4.124 ± 1.076
6.529LeuPro: 6.529 ± 2.164
2.062LeuGln: 2.062 ± 0.773
8.247LeuArg: 8.247 ± 2.206
11.34LeuSer: 11.34 ± 3.503
3.436LeuThr: 3.436 ± 1.259
7.216LeuVal: 7.216 ± 0.974
2.062LeuTrp: 2.062 ± 1.548
3.78LeuTyr: 3.78 ± 1.237
0.0LeuXaa: 0.0 ± 0.0
Met
3.436MetAla: 3.436 ± 1.2
0.344MetCys: 0.344 ± 0.779
1.718MetAsp: 1.718 ± 0.631
0.344MetGlu: 0.344 ± 0.192
1.031MetPhe: 1.031 ± 0.576
0.687MetGly: 0.687 ± 0.492
0.687MetHis: 0.687 ± 0.384
1.031MetIle: 1.031 ± 0.544
1.375MetLys: 1.375 ± 0.576
3.436MetLeu: 3.436 ± 1.272
0.0MetMet: 0.0 ± 0.0
0.344MetAsn: 0.344 ± 0.192
1.375MetPro: 1.375 ± 0.768
0.0MetGln: 0.0 ± 0.0
0.687MetArg: 0.687 ± 0.384
2.749MetSer: 2.749 ± 0.814
1.718MetThr: 1.718 ± 0.558
2.405MetVal: 2.405 ± 0.579
0.344MetTrp: 0.344 ± 0.632
0.0MetTyr: 0.0 ± 0.0
0.0MetXaa: 0.0 ± 0.0
Asn
1.375AsnAla: 1.375 ± 0.595
1.031AsnCys: 1.031 ± 0.576
1.718AsnAsp: 1.718 ± 0.96
0.344AsnGlu: 0.344 ± 0.576
2.749AsnPhe: 2.749 ± 1.054
1.718AsnGly: 1.718 ± 0.96
1.031AsnHis: 1.031 ± 0.45
1.031AsnIle: 1.031 ± 0.576
1.375AsnLys: 1.375 ± 0.595
3.78AsnLeu: 3.78 ± 1.172
1.375AsnMet: 1.375 ± 0.768
2.062AsnAsn: 2.062 ± 0.774
1.375AsnPro: 1.375 ± 0.521
0.0AsnGln: 0.0 ± 0.0
0.687AsnArg: 0.687 ± 0.488
2.062AsnSer: 2.062 ± 0.537
0.687AsnThr: 0.687 ± 0.488
2.749AsnVal: 2.749 ± 1.19
1.375AsnTrp: 1.375 ± 0.768
0.687AsnTyr: 0.687 ± 0.384
0.0AsnXaa: 0.0 ± 0.0
Pro
3.436ProAla: 3.436 ± 1.07
2.062ProCys: 2.062 ± 1.806
3.093ProAsp: 3.093 ± 1.327
2.405ProGlu: 2.405 ± 1.648
2.749ProPhe: 2.749 ± 1.989
4.124ProGly: 4.124 ± 1.038
2.405ProHis: 2.405 ± 1.555
1.718ProIle: 1.718 ± 0.96
4.811ProLys: 4.811 ± 2.793
6.873ProLeu: 6.873 ± 4.435
0.344ProMet: 0.344 ± 0.192
1.718ProAsn: 1.718 ± 0.96
2.062ProPro: 2.062 ± 1.132
2.062ProGln: 2.062 ± 0.9
2.749ProArg: 2.749 ± 1.494
5.155ProSer: 5.155 ± 1.339
2.062ProThr: 2.062 ± 1.089
6.529ProVal: 6.529 ± 2.62
0.344ProTrp: 0.344 ± 0.192
0.687ProTyr: 0.687 ± 0.558
0.0ProXaa: 0.0 ± 0.0
Gln
1.375GlnAla: 1.375 ± 0.984
0.344GlnCys: 0.344 ± 0.632
2.749GlnAsp: 2.749 ± 1.105
0.687GlnGlu: 0.687 ± 0.558
2.062GlnPhe: 2.062 ± 0.726
1.031GlnGly: 1.031 ± 0.467
0.0GlnHis: 0.0 ± 0.0
1.031GlnIle: 1.031 ± 0.45
1.031GlnLys: 1.031 ± 0.544
2.405GlnLeu: 2.405 ± 0.92
0.687GlnMet: 0.687 ± 0.384
0.0GlnAsn: 0.0 ± 0.0
0.0GlnPro: 0.0 ± 0.0
1.031GlnGln: 1.031 ± 0.576
1.031GlnArg: 1.031 ± 0.576
1.375GlnSer: 1.375 ± 0.768
2.405GlnThr: 2.405 ± 0.918
2.749GlnVal: 2.749 ± 0.807
0.344GlnTrp: 0.344 ± 0.192
0.344GlnTyr: 0.344 ± 0.192
0.0GlnXaa: 0.0 ± 0.0
Arg
2.062ArgAla: 2.062 ± 0.933
2.062ArgCys: 2.062 ± 0.774
3.78ArgAsp: 3.78 ± 1.803
2.405ArgGlu: 2.405 ± 0.935
4.124ArgPhe: 4.124 ± 1.456
2.062ArgGly: 2.062 ± 1.435
2.405ArgHis: 2.405 ± 0.935
2.405ArgIle: 2.405 ± 1.062
4.124ArgLys: 4.124 ± 2.048
5.842ArgLeu: 5.842 ± 1.272
1.031ArgMet: 1.031 ± 0.789
1.718ArgAsn: 1.718 ± 0.587
3.093ArgPro: 3.093 ± 1.63
1.031ArgGln: 1.031 ± 0.576
5.155ArgArg: 5.155 ± 1.264
4.811ArgSer: 4.811 ± 1.493
2.749ArgThr: 2.749 ± 0.974
4.467ArgVal: 4.467 ± 1.687
0.687ArgTrp: 0.687 ± 0.384
2.062ArgTyr: 2.062 ± 1.023
0.0ArgXaa: 0.0 ± 0.0
Ser
5.498SerAla: 5.498 ± 1.83
3.78SerCys: 3.78 ± 1.147
5.842SerAsp: 5.842 ± 1.736
2.405SerGlu: 2.405 ± 1.181
5.498SerPhe: 5.498 ± 1.492
3.78SerGly: 3.78 ± 1.475
1.718SerHis: 1.718 ± 0.587
3.78SerIle: 3.78 ± 1.032
4.124SerLys: 4.124 ± 1.082
10.309SerLeu: 10.309 ± 2.54
2.062SerMet: 2.062 ± 0.726
3.436SerAsn: 3.436 ± 1.001
4.467SerPro: 4.467 ± 3.126
3.093SerGln: 3.093 ± 0.894
5.842SerArg: 5.842 ± 0.467
9.622SerSer: 9.622 ± 2.7
6.529SerThr: 6.529 ± 1.723
11.34SerVal: 11.34 ± 2.291
0.687SerTrp: 0.687 ± 0.558
2.405SerTyr: 2.405 ± 1.062
0.0SerXaa: 0.0 ± 0.0
Thr
4.124ThrAla: 4.124 ± 1.416
0.687ThrCys: 0.687 ± 0.384
1.031ThrAsp: 1.031 ± 0.576
1.718ThrGlu: 1.718 ± 0.631
3.093ThrPhe: 3.093 ± 1.34
5.498ThrGly: 5.498 ± 1.569
1.031ThrHis: 1.031 ± 1.009
2.405ThrIle: 2.405 ± 0.97
1.718ThrLys: 1.718 ± 0.558
4.811ThrLeu: 4.811 ± 1.519
1.718ThrMet: 1.718 ± 0.697
0.344ThrAsn: 0.344 ± 0.192
2.062ThrPro: 2.062 ± 0.99
1.031ThrGln: 1.031 ± 0.576
3.093ThrArg: 3.093 ± 1.061
4.467ThrSer: 4.467 ± 1.544
2.405ThrThr: 2.405 ± 1.021
4.124ThrVal: 4.124 ± 1.052
0.0ThrTrp: 0.0 ± 0.0
1.718ThrTyr: 1.718 ± 0.96
0.0ThrXaa: 0.0 ± 0.0
Val
9.622ValAla: 9.622 ± 1.693
2.405ValCys: 2.405 ± 0.97
6.529ValAsp: 6.529 ± 1.095
6.529ValGlu: 6.529 ± 0.4
4.811ValPhe: 4.811 ± 0.936
5.498ValGly: 5.498 ± 1.734
2.062ValHis: 2.062 ± 0.933
2.405ValIle: 2.405 ± 0.918
5.498ValLys: 5.498 ± 1.219
5.498ValLeu: 5.498 ± 0.974
2.405ValMet: 2.405 ± 1.922
3.093ValAsn: 3.093 ± 0.841
8.591ValPro: 8.591 ± 2.967
0.687ValGln: 0.687 ± 0.384
5.842ValArg: 5.842 ± 2.102
10.309ValSer: 10.309 ± 2.06
5.842ValThr: 5.842 ± 2.39
12.027ValVal: 12.027 ± 1.771
0.0ValTrp: 0.0 ± 0.0
2.749ValTyr: 2.749 ± 0.633
0.0ValXaa: 0.0 ± 0.0
Trp
0.687TrpAla: 0.687 ± 0.488
0.344TrpCys: 0.344 ± 0.192
0.344TrpAsp: 0.344 ± 0.192
0.687TrpGlu: 0.687 ± 0.835
0.344TrpPhe: 0.344 ± 0.779
0.344TrpGly: 0.344 ± 0.192
0.0TrpHis: 0.0 ± 0.0
0.0TrpIle: 0.0 ± 0.0
1.375TrpLys: 1.375 ± 0.487
2.062TrpLeu: 2.062 ± 0.773
0.687TrpMet: 0.687 ± 0.68
0.344TrpAsn: 0.344 ± 0.192
0.344TrpPro: 0.344 ± 0.632
0.344TrpGln: 0.344 ± 0.192
0.687TrpArg: 0.687 ± 0.384
0.344TrpSer: 0.344 ± 0.576
0.344TrpThr: 0.344 ± 0.596
1.375TrpVal: 1.375 ± 0.768
0.0TrpTrp: 0.0 ± 0.0
0.344TrpTyr: 0.344 ± 0.632
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.375TyrAla: 1.375 ± 1.302
1.375TyrCys: 1.375 ± 0.595
1.031TyrAsp: 1.031 ± 0.576
1.375TyrGlu: 1.375 ± 0.768
1.718TyrPhe: 1.718 ± 0.701
1.375TyrGly: 1.375 ± 0.898
1.718TyrHis: 1.718 ± 0.935
0.687TyrIle: 0.687 ± 0.384
2.062TyrLys: 2.062 ± 0.832
4.467TyrLeu: 4.467 ± 1.933
0.344TyrMet: 0.344 ± 0.576
0.344TyrAsn: 0.344 ± 0.192
2.062TyrPro: 2.062 ± 0.537
0.344TyrGln: 0.344 ± 0.192
1.718TyrArg: 1.718 ± 1.085
2.405TyrSer: 2.405 ± 0.901
2.062TyrThr: 2.062 ± 0.774
2.405TyrVal: 2.405 ± 1.41
0.0TyrTrp: 0.0 ± 0.0
1.031TyrTyr: 1.031 ± 0.467
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 5 proteins (2911 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski