Phycisphaera sp.

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; Phycisphaerae; Phycisphaerales; Phycisphaeraceae; Phycisphaera; unclassified Phycisphaera

Average proteome isoelectric point is 5.76

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2755 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3M8FWW9|A0A3M8FWW9_9BACT Uncharacterized protein OS=Phycisphaera sp. OX=2030824 GN=ED559_12255 PE=4 SV=1
MM1 pKa = 7.0VFSHH5 pKa = 6.91AAAAQDD11 pKa = 3.91VVYY14 pKa = 10.85LPVFYY19 pKa = 11.12VGTNLSADD27 pKa = 3.43GSVVVGNQVGSYY39 pKa = 7.31EE40 pKa = 4.26TFRR43 pKa = 11.84WTASSGPVLLGEE55 pKa = 4.11ATAPVLGVGAGGPDD69 pKa = 3.41VSHH72 pKa = 7.65DD73 pKa = 3.75GTRR76 pKa = 11.84VSATILTEE84 pKa = 4.27DD85 pKa = 3.84ATLATPGFWTQGLGWTRR102 pKa = 11.84CMPPMAPDD110 pKa = 4.17GRR112 pKa = 11.84ILDD115 pKa = 3.89QAIGSAWGISGDD127 pKa = 3.47GTTVSGLHH135 pKa = 6.31WYY137 pKa = 10.56SMPDD141 pKa = 3.08GSYY144 pKa = 10.64GGARR148 pKa = 11.84PFTWSASSGLIQLDD162 pKa = 3.22ITAGQDD168 pKa = 2.94GRR170 pKa = 11.84ANTTNLDD177 pKa = 3.33GSVAVGWEE185 pKa = 4.28SNAFGHH191 pKa = 5.7WQPVVWRR198 pKa = 11.84NGTRR202 pKa = 11.84MQLSEE207 pKa = 3.75NDD209 pKa = 3.33AFVGCEE215 pKa = 3.73EE216 pKa = 4.26VTSDD220 pKa = 3.08GNTVVGQSWYY230 pKa = 10.2EE231 pKa = 3.64PTLNRR236 pKa = 11.84VATVWAWNGSSYY248 pKa = 11.18DD249 pKa = 3.77QNQLGVLPGTPVDD262 pKa = 4.52FGSSVAFGVSDD273 pKa = 4.38DD274 pKa = 3.47GSMIVGVNRR283 pKa = 11.84FAVSPGGPSKK293 pKa = 11.12GFIWTAEE300 pKa = 3.89TGMVEE305 pKa = 4.18AEE307 pKa = 4.83AYY309 pKa = 10.48LADD312 pKa = 4.5LGIDD316 pKa = 3.57VSDD319 pKa = 3.82EE320 pKa = 3.73MDD322 pKa = 3.54LRR324 pKa = 11.84SVDD327 pKa = 4.36GISADD332 pKa = 3.19GSTILISGWDD342 pKa = 3.48SATQIIKK349 pKa = 10.46SAIVTLTTPCVADD362 pKa = 3.7TNGDD366 pKa = 3.84GQLTPADD373 pKa = 3.53FTAWINAFNNALPEE387 pKa = 4.44CDD389 pKa = 3.44QNADD393 pKa = 3.92GACTPTDD400 pKa = 3.73FTAWINNYY408 pKa = 9.49NAGCDD413 pKa = 3.3

Molecular weight: 43.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3M8FWU6|A0A3M8FWU6_9BACT FHA domain-containing protein OS=Phycisphaera sp. OX=2030824 GN=ED559_11140 PE=3 SV=1
MM1 pKa = 7.08VHH3 pKa = 6.64TSPDD7 pKa = 3.04SSYY10 pKa = 11.88DD11 pKa = 3.83CLKK14 pKa = 10.3HH15 pKa = 6.13ACTRR19 pKa = 11.84RR20 pKa = 11.84PHH22 pKa = 6.46RR23 pKa = 11.84GSHH26 pKa = 4.13GRR28 pKa = 11.84IRR30 pKa = 11.84TRR32 pKa = 11.84TKK34 pKa = 9.89RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.66HH38 pKa = 4.4RR39 pKa = 11.84WQRR42 pKa = 11.84KK43 pKa = 7.29LLARR47 pKa = 11.84KK48 pKa = 8.75PRR50 pKa = 11.84GRR52 pKa = 11.84KK53 pKa = 8.23QLVRR57 pKa = 11.84RR58 pKa = 11.84LLRR61 pKa = 11.84RR62 pKa = 11.84PRR64 pKa = 11.84GRR66 pKa = 11.84RR67 pKa = 11.84PLARR71 pKa = 11.84RR72 pKa = 11.84RR73 pKa = 11.84PARR76 pKa = 11.84RR77 pKa = 11.84QPARR81 pKa = 11.84RR82 pKa = 11.84QPARR86 pKa = 11.84RR87 pKa = 11.84QPARR91 pKa = 11.84RR92 pKa = 11.84PLARR96 pKa = 11.84RR97 pKa = 11.84PLARR101 pKa = 11.84RR102 pKa = 11.84PLARR106 pKa = 11.84RR107 pKa = 11.84PLARR111 pKa = 11.84RR112 pKa = 11.84PLARR116 pKa = 11.84RR117 pKa = 11.84PLARR121 pKa = 11.84RR122 pKa = 11.84PPARR126 pKa = 11.84RR127 pKa = 11.84RR128 pKa = 11.84PARR131 pKa = 11.84RR132 pKa = 11.84RR133 pKa = 11.84PARR136 pKa = 11.84RR137 pKa = 11.84PLARR141 pKa = 11.84RR142 pKa = 11.84PLARR146 pKa = 11.84RR147 pKa = 11.84RR148 pKa = 11.84PARR151 pKa = 11.84RR152 pKa = 11.84PLARR156 pKa = 11.84RR157 pKa = 11.84QPARR161 pKa = 11.84RR162 pKa = 11.84PLARR166 pKa = 11.84RR167 pKa = 11.84RR168 pKa = 11.84PARR171 pKa = 11.84RR172 pKa = 11.84PLAAAKK178 pKa = 10.34ANRR181 pKa = 11.84LSDD184 pKa = 3.99RR185 pKa = 11.84LSLSPILMLNLAAGPSGPAVFSCARR210 pKa = 11.84GRR212 pKa = 11.84ALFVRR217 pKa = 11.84DD218 pKa = 3.43

Molecular weight: 25.75 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2755

0

2755

989657

31

4552

359.2

39.17

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.218 ± 0.053

1.033 ± 0.016

6.561 ± 0.055

6.654 ± 0.053

3.496 ± 0.03

8.445 ± 0.054

2.044 ± 0.029

5.506 ± 0.033

3.263 ± 0.044

9.341 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.357 ± 0.027

3.081 ± 0.039

4.948 ± 0.033

3.279 ± 0.027

6.54 ± 0.046

6.522 ± 0.038

5.749 ± 0.043

7.395 ± 0.035

1.326 ± 0.022

2.241 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski