Proteome-pI 2.0 - Proteome Isoelectric Point Database

Lysinibacillus odysseyi 34hs-1 = NBRC 100172

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Lysinibacillus; Lysinibacillus odysseyi

Average proteome isoelectric point is 6.08

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 4109 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>tr|A0A0A3IDT0|A0A0A3IDT0_9BACI Uncharacterized protein OS=Lysinibacillus odysseyi 34hs-1 = NBRC 100172 OX=1220589 GN=CD32_19010 PE=4 SV=1

MM1 pKa = 7.59EE2 pKa = 5.56LVQIGDD8 pKa = 3.52WEE10 pKa = 4.51LLVDD14 pKa = 4.14IPKK17 pKa = 9.63TKK19 pKa = 10.68AFYY22 pKa = 10.71EE23 pKa = 4.15QMDD26 pKa = 3.75VQNDD30 pKa = 3.08ILHH33 pKa = 6.04WLNYY37 pKa = 9.85IEE39 pKa = 4.61ISSFIDD45 pKa = 3.61LEE47 pKa = 3.95IQAFFDD53 pKa = 3.58SFGIDD58 pKa = 2.62IVKK61 pKa = 10.07PSNLSCHH68 pKa = 5.48PVEE71 pKa = 5.71DD72 pKa = 4.54GSKK75 pKa = 9.35MMYY78 pKa = 8.38TGSYY82 pKa = 8.92HH83 pKa = 7.71LYY85 pKa = 9.77GEE87 pKa = 4.28RR88 pKa = 11.84LKK90 pKa = 11.55GEE92 pKa = 4.23LDD94 pKa = 3.2GWDD97 pKa = 4.93LIVGDD102 pKa = 4.32YY103 pKa = 10.48CFSITTEE110 pKa = 3.97MEE112 pKa = 4.11AVPEE116 pKa = 4.11GMNGNITEE124 pKa = 4.02ISFEE128 pKa = 4.3VVLPWMLPLPISSMM142 pKa = 3.69

Molecular weight: 16.25 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|A0A0A3IVS0|A0A0A3IVS0_9BACI Succinate--CoA ligase [ADP-forming] subunit beta OS=Lysinibacillus odysseyi 34hs-1 = NBRC 100172 OX=1220589 GN=sucC PE=3 SV=1

MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.14QPSKK9 pKa = 9.1RR10 pKa = 11.84KK11 pKa = 9.07HH12 pKa = 5.9SKK14 pKa = 8.59VHH16 pKa = 5.68GFRR19 pKa = 11.84ARR21 pKa = 11.84MSTKK25 pKa = 10.19NGRR28 pKa = 11.84KK29 pKa = 8.91VLAARR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 8.81GRR39 pKa = 11.84KK40 pKa = 8.75VLSAA44 pKa = 4.05

Molecular weight: 5.17 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
4109	0	4109	1154994	37	1976	281.1	31.55

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
7.748 ± 0.039	0.738 ± 0.012	4.947 ± 0.027	7.81 ± 0.043	4.565 ± 0.035	6.711 ± 0.039	2.081 ± 0.019	7.809 ± 0.041	6.477 ± 0.028	9.803 ± 0.044

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
2.892 ± 0.018	4.104 ± 0.031	3.509 ± 0.019	3.855 ± 0.028	4.041 ± 0.028	5.641 ± 0.026	5.563 ± 0.031	7.011 ± 0.031	0.986 ± 0.014	3.709 ± 0.026

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski