Cassava associated gemycircularvirus 1

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemycircularvirus; Gemycircularvirus cassa1

Average proteome isoelectric point is 7.46

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|I0AY94|I0AY94_9VIRU Capsid protein OS=Cassava associated gemycircularvirus 1 OX=1985374 PE=4 SV=1
MM1 pKa = 7.81PFYY4 pKa = 11.01FNARR8 pKa = 11.84YY9 pKa = 9.8ALITYY14 pKa = 7.34AQCDD18 pKa = 3.64ALDD21 pKa = 3.81GFRR24 pKa = 11.84VMDD27 pKa = 4.02HH28 pKa = 6.63FSGLGAEE35 pKa = 4.67CIIARR40 pKa = 11.84EE41 pKa = 4.0IHH43 pKa = 5.9QDD45 pKa = 3.21GGVHH49 pKa = 5.75LHH51 pKa = 6.35CFIDD55 pKa = 4.11FGRR58 pKa = 11.84KK59 pKa = 7.81FRR61 pKa = 11.84SRR63 pKa = 11.84RR64 pKa = 11.84TDD66 pKa = 2.64IFDD69 pKa = 3.14VDD71 pKa = 3.48GRR73 pKa = 11.84HH74 pKa = 6.37PNIEE78 pKa = 4.28PSWGTPWRR86 pKa = 11.84GYY88 pKa = 11.06DD89 pKa = 3.55YY90 pKa = 10.79AIKK93 pKa = 10.71DD94 pKa = 3.36GDD96 pKa = 4.29VICGGLEE103 pKa = 3.69RR104 pKa = 11.84PEE106 pKa = 3.96QPRR109 pKa = 11.84SEE111 pKa = 4.04RR112 pKa = 11.84VKK114 pKa = 10.93KK115 pKa = 10.61DD116 pKa = 2.57WDD118 pKa = 3.12QWAEE122 pKa = 3.65ITNARR127 pKa = 11.84DD128 pKa = 3.31RR129 pKa = 11.84DD130 pKa = 4.16HH131 pKa = 6.74FWEE134 pKa = 4.84LVHH137 pKa = 7.94HH138 pKa = 7.16LDD140 pKa = 4.88PKK142 pKa = 10.88AAACNYY148 pKa = 8.82GQLAKK153 pKa = 10.78YY154 pKa = 10.3ADD156 pKa = 3.4WRR158 pKa = 11.84FASVPPVYY166 pKa = 9.8EE167 pKa = 4.19SPGGISFIGGDD178 pKa = 3.39VDD180 pKa = 6.35GRR182 pKa = 11.84DD183 pKa = 3.28AWCEE187 pKa = 3.6QSHH190 pKa = 6.07IRR192 pKa = 11.84SGQPLPGRR200 pKa = 11.84CMSLVLYY207 pKa = 10.59GDD209 pKa = 3.49SRR211 pKa = 11.84TGKK214 pKa = 7.28TLWARR219 pKa = 11.84SLGTHH224 pKa = 7.44LYY226 pKa = 8.09TVGMVSGEE234 pKa = 4.09EE235 pKa = 3.83LKK237 pKa = 10.84KK238 pKa = 11.02ASNDD242 pKa = 3.4DD243 pKa = 3.12VKK245 pKa = 11.37YY246 pKa = 10.88AIFDD250 pKa = 4.73DD251 pKa = 3.41IRR253 pKa = 11.84GGIKK257 pKa = 9.97FFPAFKK263 pKa = 9.97EE264 pKa = 3.94WLGAQAYY271 pKa = 6.64VTVKK275 pKa = 10.24EE276 pKa = 4.66LYY278 pKa = 10.11RR279 pKa = 11.84EE280 pKa = 4.09PKK282 pKa = 8.72LVKK285 pKa = 9.08WGKK288 pKa = 8.36PSIWISNDD296 pKa = 3.14DD297 pKa = 3.81PRR299 pKa = 11.84LGMDD303 pKa = 3.57ASDD306 pKa = 4.25VSWLEE311 pKa = 3.81HH312 pKa = 4.93NCYY315 pKa = 9.61FVEE318 pKa = 4.32VSSPIFRR325 pKa = 11.84ANTEE329 pKa = 3.84

Molecular weight: 37.55 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|I0AY94|I0AY94_9VIRU Capsid protein OS=Cassava associated gemycircularvirus 1 OX=1985374 PE=4 SV=1
MM1 pKa = 7.45PRR3 pKa = 11.84ATRR6 pKa = 11.84TSDD9 pKa = 2.71RR10 pKa = 11.84LGRR13 pKa = 11.84GRR15 pKa = 11.84RR16 pKa = 11.84RR17 pKa = 11.84STRR20 pKa = 11.84RR21 pKa = 11.84SSRR24 pKa = 11.84RR25 pKa = 11.84SVAKK29 pKa = 9.83RR30 pKa = 11.84PPYY33 pKa = 10.0RR34 pKa = 11.84KK35 pKa = 9.58YY36 pKa = 10.22LRR38 pKa = 11.84KK39 pKa = 8.54MSKK42 pKa = 10.01RR43 pKa = 11.84RR44 pKa = 11.84ILNITSTKK52 pKa = 10.55KK53 pKa = 9.82KK54 pKa = 10.02DD55 pKa = 3.3TMTGYY60 pKa = 9.34TNSTPANQTGGTVYY74 pKa = 9.77STDD77 pKa = 3.49PAIVTGGLNANQCNAFLWCATARR100 pKa = 11.84TNQVINPTQNTLVPGNKK117 pKa = 9.38FYY119 pKa = 9.93TACRR123 pKa = 11.84TDD125 pKa = 3.35SNPYY129 pKa = 9.07MVGLSEE135 pKa = 5.66KK136 pKa = 10.46IEE138 pKa = 4.03IQCNSGMPWQWRR150 pKa = 11.84RR151 pKa = 11.84ICWTQKK157 pKa = 10.34GGQGLPANSTSFNLSQQNAITGWNRR182 pKa = 11.84VMNQVAGNPGTGDD195 pKa = 3.28QYY197 pKa = 12.13NLFHH201 pKa = 7.06HH202 pKa = 7.12IFRR205 pKa = 11.84GQVGSDD211 pKa = 2.67WVDD214 pKa = 3.13VMTAPLDD221 pKa = 3.95PQHH224 pKa = 6.41ITVKK228 pKa = 9.45YY229 pKa = 10.02DD230 pKa = 3.29KK231 pKa = 10.99VVTLASGNEE240 pKa = 3.82EE241 pKa = 4.11GFIRR245 pKa = 11.84AYY247 pKa = 10.5RR248 pKa = 11.84RR249 pKa = 11.84WHH251 pKa = 5.98PMKK254 pKa = 10.01KK255 pKa = 7.35TLSYY259 pKa = 11.38ADD261 pKa = 3.68EE262 pKa = 4.69EE263 pKa = 4.68YY264 pKa = 11.53GNVEE268 pKa = 3.77NADD271 pKa = 3.64AFSQVGKK278 pKa = 10.64KK279 pKa = 10.81GMGDD283 pKa = 3.83YY284 pKa = 10.92YY285 pKa = 11.62VLDD288 pKa = 4.21LFRR291 pKa = 11.84ARR293 pKa = 11.84QGAQTNDD300 pKa = 3.66SISIRR305 pKa = 11.84PCATLYY311 pKa = 9.43WHH313 pKa = 7.09EE314 pKa = 4.25KK315 pKa = 9.3

Molecular weight: 35.61 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

644

315

329

322.0

36.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.988 ± 0.227

2.174 ± 0.19

6.677 ± 1.576

4.037 ± 1.057

3.882 ± 0.947

8.696 ± 0.312

2.484 ± 0.633

4.969 ± 0.594

5.124 ± 0.192

5.59 ± 0.36

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.174 ± 0.482

4.814 ± 1.756

4.969 ± 0.146

3.882 ± 1.069

8.075 ± 0.575

6.366 ± 0.212

6.056 ± 2.224

5.59 ± 0.136

3.106 ± 0.399

4.348 ± 0.068
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski