Escherichia phage ECA2

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Teetrevirus; Escherichia virus ECA2

Average proteome isoelectric point is 6.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 47 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A193GYZ0|A0A193GYZ0_9CAUD Endonuclease VII OS=Escherichia phage ECA2 OX=1852630 PE=4 SV=1
MM1 pKa = 7.48AMSNMTYY8 pKa = 11.02SNVFAHH14 pKa = 7.22AYY16 pKa = 9.93EE17 pKa = 4.05MLNEE21 pKa = 4.3NIRR24 pKa = 11.84YY25 pKa = 9.62DD26 pKa = 3.71DD27 pKa = 3.64TRR29 pKa = 11.84DD30 pKa = 3.33TDD32 pKa = 3.85GLSDD36 pKa = 5.53AIHH39 pKa = 6.42EE40 pKa = 4.65AADD43 pKa = 3.63SAVPHH48 pKa = 6.4YY49 pKa = 10.5YY50 pKa = 10.65SDD52 pKa = 3.44IFSVMASDD60 pKa = 5.8GIDD63 pKa = 4.0LEE65 pKa = 4.94FEE67 pKa = 4.75DD68 pKa = 5.02SGLMPDD74 pKa = 4.21TKK76 pKa = 10.95DD77 pKa = 3.3VTCILQARR85 pKa = 11.84IYY87 pKa = 9.36EE88 pKa = 4.14QLTIDD93 pKa = 3.75LWEE96 pKa = 4.32KK97 pKa = 11.36AEE99 pKa = 4.51DD100 pKa = 3.81LLNEE104 pKa = 3.94YY105 pKa = 10.53LEE107 pKa = 4.67EE108 pKa = 5.07IEE110 pKa = 4.4EE111 pKa = 4.2EE112 pKa = 4.18EE113 pKa = 4.53EE114 pKa = 3.94EE115 pKa = 4.41VEE117 pKa = 4.12EE118 pKa = 5.49DD119 pKa = 3.84EE120 pKa = 4.93EE121 pKa = 4.44

Molecular weight: 13.99 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A193GYP0|A0A193GYP0_9CAUD Terminase large subunit OS=Escherichia phage ECA2 OX=1852630 PE=3 SV=1
MM1 pKa = 6.77MAALIALVYY10 pKa = 10.28LCIAMYY16 pKa = 9.72FMRR19 pKa = 11.84DD20 pKa = 3.72FKK22 pKa = 11.01RR23 pKa = 11.84GLAIHH28 pKa = 7.05KK29 pKa = 7.75ATLSYY34 pKa = 9.75FKK36 pKa = 10.03WGFIPRR42 pKa = 11.84FTVRR46 pKa = 11.84EE47 pKa = 3.73RR48 pKa = 11.84NGRR51 pKa = 11.84FKK53 pKa = 11.15ANKK56 pKa = 9.52VGIFHH61 pKa = 7.52IATHH65 pKa = 6.2

Molecular weight: 7.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

47

0

47

11410

37

1320

242.8

27.14

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.369 ± 0.419

0.92 ± 0.202

6.494 ± 0.25

7.222 ± 0.418

3.655 ± 0.232

7.362 ± 0.342

1.946 ± 0.234

5.259 ± 0.231

6.722 ± 0.401

7.967 ± 0.372

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.954 ± 0.201

4.531 ± 0.38

3.523 ± 0.197

3.953 ± 0.31

5.697 ± 0.246

5.784 ± 0.377

5.557 ± 0.329

6.31 ± 0.301

1.49 ± 0.172

3.287 ± 0.175
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski