Lactobacillus phage Nyseid

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Tybeckvirinae; Lenusvirus; Lactobacillus virus Nyseid

Average proteome isoelectric point is 5.83

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 125 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2K9VCF4|A0A2K9VCF4_9CAUD Uncharacterized protein OS=Lactobacillus phage Nyseid OX=2079432 PE=4 SV=1
MM1 pKa = 7.94EE2 pKa = 5.41DD3 pKa = 3.75TDD5 pKa = 3.66EE6 pKa = 4.32MLYY9 pKa = 10.36QATKK13 pKa = 10.02AANGFDD19 pKa = 3.14WTFDD23 pKa = 3.02LRR25 pKa = 11.84AAIEE29 pKa = 4.58YY30 pKa = 8.18YY31 pKa = 9.86TDD33 pKa = 3.23VYY35 pKa = 8.44MTSYY39 pKa = 11.54DD40 pKa = 3.65EE41 pKa = 5.07AIKK44 pKa = 10.7NGKK47 pKa = 6.7TEE49 pKa = 4.18EE50 pKa = 4.2EE51 pKa = 4.33SSQIAQNLLIVMMEE65 pKa = 4.12YY66 pKa = 11.05GDD68 pKa = 4.45QINGDD73 pKa = 3.47TDD75 pKa = 3.43EE76 pKa = 4.35

Molecular weight: 8.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2K9VC41|A0A2K9VC41_9CAUD Uncharacterized protein OS=Lactobacillus phage Nyseid OX=2079432 PE=4 SV=1
MM1 pKa = 7.64KK2 pKa = 10.16FYY4 pKa = 11.01KK5 pKa = 10.59LLIAIIASVTLSLVTAYY22 pKa = 10.72NVDD25 pKa = 3.53ASQKK29 pKa = 9.64PNYY32 pKa = 9.96AYY34 pKa = 9.73TLIRR38 pKa = 11.84TKK40 pKa = 10.26QHH42 pKa = 6.72AIKK45 pKa = 9.24LTNTGRR51 pKa = 11.84TEE53 pKa = 3.42NMYY56 pKa = 10.51RR57 pKa = 11.84VTIKK61 pKa = 10.26QRR63 pKa = 11.84NHH65 pKa = 5.8SKK67 pKa = 9.4WYY69 pKa = 10.44YY70 pKa = 7.45MALNARR76 pKa = 11.84QSWTVNQSGKK86 pKa = 10.46YY87 pKa = 8.17SVTVRR92 pKa = 11.84RR93 pKa = 11.84VSKK96 pKa = 10.88VDD98 pKa = 3.31EE99 pKa = 4.65KK100 pKa = 11.42RR101 pKa = 11.84NADD104 pKa = 3.65PRR106 pKa = 11.84NHH108 pKa = 7.3FIPLGISNTQRR119 pKa = 11.84TIWNRR124 pKa = 3.1

Molecular weight: 14.52 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

125

0

125

23425

41

1886

187.4

21.11

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.959 ± 0.521

0.756 ± 0.104

7.326 ± 0.431

5.836 ± 0.283

3.876 ± 0.19

7.065 ± 0.41

1.729 ± 0.124

7.065 ± 0.228

8.179 ± 0.351

7.782 ± 0.196

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.736 ± 0.11

6.502 ± 0.196

2.63 ± 0.153

3.326 ± 0.155

3.419 ± 0.173

7.261 ± 0.327

6.006 ± 0.344

6.523 ± 0.216

1.289 ± 0.114

4.734 ± 0.275
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski