Exiguobacterium phage vB_EauM-23

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 66 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A482D0V6|A0A482D0V6_9CAUD Uncharacterized protein OS=Exiguobacterium phage vB_EauM-23 OX=2546569 GN=EauM23_00039 PE=4 SV=1
MM1 pKa = 6.9QQTEE5 pKa = 4.03QFGYY9 pKa = 10.52IDD11 pKa = 3.87IDD13 pKa = 3.58KK14 pKa = 11.14ALIPYY19 pKa = 10.23RR20 pKa = 11.84FDD22 pKa = 2.82ITLAGEE28 pKa = 4.17VFFFDD33 pKa = 3.57VRR35 pKa = 11.84YY36 pKa = 10.38AEE38 pKa = 3.97PTGIITVDD46 pKa = 4.04LYY48 pKa = 11.39DD49 pKa = 4.57ADD51 pKa = 4.04EE52 pKa = 4.73DD53 pKa = 4.88PIVFGEE59 pKa = 3.96PLIYY63 pKa = 10.13GQPLFDD69 pKa = 4.42DD70 pKa = 4.35VVDD73 pKa = 4.09EE74 pKa = 4.73RR75 pKa = 11.84LPLNQLVPFDD85 pKa = 4.5PSGKK89 pKa = 9.08SSRR92 pKa = 11.84VTFDD96 pKa = 3.28NLGDD100 pKa = 3.83TVFLFDD106 pKa = 3.48VSEE109 pKa = 4.72PIIEE113 pKa = 4.55DD114 pKa = 3.57EE115 pKa = 4.95GSGALL120 pKa = 4.12

Molecular weight: 13.49 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A482CVL6|A0A482CVL6_9CAUD Uncharacterized protein OS=Exiguobacterium phage vB_EauM-23 OX=2546569 GN=EauM23_00007 PE=4 SV=1
MM1 pKa = 7.43KK2 pKa = 9.43MAFEE6 pKa = 4.49IVSFTAVIMLFAFCFVALFAHH27 pKa = 5.67QWWMVLVGGLGLGLLLLMEE46 pKa = 4.62YY47 pKa = 10.84ARR49 pKa = 11.84DD50 pKa = 3.43RR51 pKa = 11.84RR52 pKa = 11.84SARR55 pKa = 11.84RR56 pKa = 11.84RR57 pKa = 11.84RR58 pKa = 11.84EE59 pKa = 3.68YY60 pKa = 10.7WNGTGLL66 pKa = 3.96

Molecular weight: 7.72 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

66

0

66

11451

32

809

173.5

19.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.043 ± 0.514

0.568 ± 0.131

6.052 ± 0.28

7.79 ± 0.525

4.0 ± 0.167

7.362 ± 0.393

1.659 ± 0.196

6.061 ± 0.213

6.847 ± 0.439

7.729 ± 0.262

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.882 ± 0.206

4.576 ± 0.237

3.039 ± 0.201

3.633 ± 0.187

5.72 ± 0.364

5.991 ± 0.459

6.218 ± 0.356

7.004 ± 0.343

1.24 ± 0.139

3.589 ± 0.271
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski