Bythopirellula polymerisocia

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; Planctomycetia; Pirellulales; Lacipirellulaceae; Bythopirellula

Average proteome isoelectric point is 5.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5040 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5C6D0F5|A0A5C6D0F5_9BACT Uncharacterized protein OS=Bythopirellula polymerisocia OX=2528003 GN=Pla144_11360 PE=4 SV=1
MM1 pKa = 7.34SKK3 pKa = 9.88ILAILCFGFLAAAVMSDD20 pKa = 4.14RR21 pKa = 11.84MDD23 pKa = 4.32LKK25 pKa = 11.3LLDD28 pKa = 4.07SDD30 pKa = 5.28SFAHH34 pKa = 6.79CGKK37 pKa = 10.59CGDD40 pKa = 4.31GDD42 pKa = 4.18AEE44 pKa = 5.02DD45 pKa = 4.23DD46 pKa = 4.38HH47 pKa = 7.75EE48 pKa = 6.22HH49 pKa = 6.65EE50 pKa = 5.4GDD52 pKa = 3.71DD53 pKa = 3.65EE54 pKa = 4.72EE55 pKa = 6.37SFVHH59 pKa = 6.81CGKK62 pKa = 10.78CGDD65 pKa = 4.22GDD67 pKa = 4.18AEE69 pKa = 5.02DD70 pKa = 4.23DD71 pKa = 4.38HH72 pKa = 7.75EE73 pKa = 6.22HH74 pKa = 6.65EE75 pKa = 5.4GDD77 pKa = 3.71DD78 pKa = 3.65EE79 pKa = 4.72EE80 pKa = 6.37SFVHH84 pKa = 6.81CGKK87 pKa = 10.78CGDD90 pKa = 4.22GDD92 pKa = 4.18AEE94 pKa = 5.3DD95 pKa = 4.65DD96 pKa = 4.38HH97 pKa = 7.86EE98 pKa = 6.69HH99 pKa = 7.47DD100 pKa = 4.94GDD102 pKa = 4.02DD103 pKa = 3.59EE104 pKa = 4.65EE105 pKa = 6.41SFVHH109 pKa = 6.81CGKK112 pKa = 10.78CGDD115 pKa = 4.22GDD117 pKa = 4.18AEE119 pKa = 5.02DD120 pKa = 4.23DD121 pKa = 4.38HH122 pKa = 7.75EE123 pKa = 6.22HH124 pKa = 6.65EE125 pKa = 5.4GDD127 pKa = 3.71DD128 pKa = 3.65EE129 pKa = 4.72EE130 pKa = 6.37SFVHH134 pKa = 6.81CGKK137 pKa = 10.78CGDD140 pKa = 4.22GDD142 pKa = 4.22AEE144 pKa = 5.12DD145 pKa = 5.44DD146 pKa = 4.32HH147 pKa = 8.98DD148 pKa = 5.81HH149 pKa = 7.82DD150 pKa = 5.85DD151 pKa = 4.44DD152 pKa = 4.49EE153 pKa = 4.96EE154 pKa = 5.42SFVV157 pKa = 3.79

Molecular weight: 17.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5C6CAB7|A0A5C6CAB7_9BACT Heparinase II/III-like protein OS=Bythopirellula polymerisocia OX=2528003 GN=Pla144_49910 PE=4 SV=1
MM1 pKa = 7.49SCLSAARR8 pKa = 11.84RR9 pKa = 11.84RR10 pKa = 11.84TGTDD14 pKa = 3.11SEE16 pKa = 4.61SSGWKK21 pKa = 9.3RR22 pKa = 11.84VPATNPYY29 pKa = 11.13ADD31 pKa = 3.45ITHH34 pKa = 7.31LFSALLTARR43 pKa = 11.84RR44 pKa = 11.84RR45 pKa = 11.84QTMPIRR51 pKa = 11.84CAQCANPHH59 pKa = 6.47AEE61 pKa = 3.94HH62 pKa = 7.07CPRR65 pKa = 11.84CALLARR71 pKa = 11.84SAGSSTSQFNKK82 pKa = 9.74VQITCPGRR90 pKa = 11.84GWHH93 pKa = 6.41IEE95 pKa = 3.7KK96 pKa = 10.46SYY98 pKa = 10.91QEE100 pKa = 4.34PGNTVQTLIDD110 pKa = 3.6STLFLAEE117 pKa = 4.22VNLTAARR124 pKa = 11.84YY125 pKa = 8.63GRR127 pKa = 11.84RR128 pKa = 11.84LGASACQTPKK138 pKa = 10.61LSRR141 pKa = 11.84GYY143 pKa = 9.86RR144 pKa = 11.84KK145 pKa = 10.13SKK147 pKa = 10.05NPPYY151 pKa = 10.62LYY153 pKa = 10.92ANLSRR158 pKa = 11.84PMKK161 pKa = 9.98PKK163 pKa = 10.62LPSEE167 pKa = 4.22LSLPRR172 pKa = 11.84RR173 pKa = 11.84SVDD176 pKa = 3.27RR177 pKa = 11.84RR178 pKa = 11.84HH179 pKa = 5.24LTIARR184 pKa = 11.84VIEE187 pKa = 4.01LVLCVSSSSSLFMVRR202 pKa = 4.64

Molecular weight: 22.46 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5040

0

5040

1770376

29

3734

351.3

38.63

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.294 ± 0.036

1.185 ± 0.015

5.73 ± 0.027

6.273 ± 0.036

3.93 ± 0.021

7.963 ± 0.046

2.144 ± 0.019

5.214 ± 0.019

3.604 ± 0.029

10.192 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.173 ± 0.014

3.654 ± 0.03

4.99 ± 0.026

4.043 ± 0.022

5.931 ± 0.034

6.724 ± 0.032

5.638 ± 0.031

7.182 ± 0.029

1.551 ± 0.018

2.586 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski