Subdoligranulum variabile DSM 15176

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Oscillospiraceae; Subdoligranulum; Subdoligranulum variabile

Average proteome isoelectric point is 6.41

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3378 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|D1PJT2|D1PJT2_9FIRM Transcriptional regulator GntR family OS=Subdoligranulum variabile DSM 15176 OX=411471 GN=SUBVAR_04652 PE=4 SV=1
MM1 pKa = 7.59KK2 pKa = 10.36LKK4 pKa = 9.41KK5 pKa = 8.93TLSLCLAALLTAGLVGCGSTATTDD29 pKa = 3.02STAASDD35 pKa = 4.06SAASTAEE42 pKa = 4.06TGTEE46 pKa = 4.09ADD48 pKa = 3.43TTAAPDD54 pKa = 3.75GDD56 pKa = 4.38GDD58 pKa = 4.1PTTLTVAMEE67 pKa = 4.41CAYY70 pKa = 10.47APYY73 pKa = 10.74NWTQSDD79 pKa = 3.8DD80 pKa = 3.89SNGAVQIRR88 pKa = 11.84DD89 pKa = 3.59SSDD92 pKa = 2.9YY93 pKa = 11.09AYY95 pKa = 10.51GYY97 pKa = 11.18DD98 pKa = 3.3VMMAKK103 pKa = 10.3KK104 pKa = 9.65IAEE107 pKa = 3.95ALGQNVQVVKK117 pKa = 10.88LDD119 pKa = 3.39WDD121 pKa = 3.96SLIPAVMSGDD131 pKa = 3.72VDD133 pKa = 3.99CVIAGQSITSEE144 pKa = 3.93RR145 pKa = 11.84AQQVDD150 pKa = 3.88FSDD153 pKa = 4.25PYY155 pKa = 10.26YY156 pKa = 9.22YY157 pKa = 11.11ASIITLVKK165 pKa = 10.34KK166 pKa = 10.54DD167 pKa = 3.67GPYY170 pKa = 11.29ANAKK174 pKa = 9.75SVADD178 pKa = 4.31LSGATCTSQLGTIWYY193 pKa = 7.85DD194 pKa = 2.92NCLPQIPDD202 pKa = 3.6ANILPAQEE210 pKa = 4.08TAPAMLVALNSGACDD225 pKa = 3.07VVVTDD230 pKa = 5.34RR231 pKa = 11.84PTAQAALVAYY241 pKa = 8.86PDD243 pKa = 4.48FVALDD248 pKa = 3.74FGGGDD253 pKa = 3.61EE254 pKa = 4.85DD255 pKa = 4.57FQVSDD260 pKa = 3.62EE261 pKa = 5.31DD262 pKa = 3.87INIGISMKK270 pKa = 10.51KK271 pKa = 10.54GNTALKK277 pKa = 10.44DD278 pKa = 4.02AINGVLATMTTDD290 pKa = 4.71DD291 pKa = 4.84YY292 pKa = 11.44NTMMDD297 pKa = 3.73EE298 pKa = 4.9AISVQPLSEE307 pKa = 3.96

Molecular weight: 32.09 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|D1PRN7|D1PRN7_9FIRM Ribonuclease P protein component OS=Subdoligranulum variabile DSM 15176 OX=411471 GN=rnpA PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.6RR3 pKa = 11.84TFQPKK8 pKa = 8.37KK9 pKa = 7.49RR10 pKa = 11.84QRR12 pKa = 11.84SRR14 pKa = 11.84VHH16 pKa = 6.45GFLQRR21 pKa = 11.84MSTKK25 pKa = 10.36NGRR28 pKa = 11.84KK29 pKa = 8.58VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.04GRR39 pKa = 11.84KK40 pKa = 8.89SLTVV44 pKa = 3.12

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3378

0

3378

991092

35

2992

293.4

32.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.162 ± 0.051

1.767 ± 0.019

5.561 ± 0.04

6.214 ± 0.046

3.854 ± 0.029

7.635 ± 0.041

1.949 ± 0.021

5.29 ± 0.043

4.586 ± 0.045

10.161 ± 0.056

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.684 ± 0.022

3.35 ± 0.027

4.414 ± 0.031

3.853 ± 0.029

5.61 ± 0.043

5.152 ± 0.033

5.848 ± 0.043

7.066 ± 0.034

1.199 ± 0.017

3.645 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski