Kallithea virus

Taxonomy: Viruses; Naldaviricetes; Lefavirales; Nudiviridae; Alphanudivirus; Drosophila melanogaster nudivirus A

Average proteome isoelectric point is 6.7

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 95 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1S5VFX8|A0A1S5VFX8_9VIRU Uncharacterized protein OS=Kallithea virus OX=1654582 PE=4 SV=1
MM1 pKa = 7.23NVADD5 pKa = 4.55TPIGYY10 pKa = 8.91PNGLDD15 pKa = 3.43PNIQKK20 pKa = 9.79SQNVDD25 pKa = 3.49NIEE28 pKa = 4.27SGVTSINNNIDD39 pKa = 2.93NTTRR43 pKa = 11.84QRR45 pKa = 11.84ILYY48 pKa = 8.48ALQRR52 pKa = 11.84AIPDD56 pKa = 3.36LPKK59 pKa = 10.31IITEE63 pKa = 4.16LTNQKK68 pKa = 8.49HH69 pKa = 4.45TTPEE73 pKa = 3.94FEE75 pKa = 5.13LNNIVDD81 pKa = 3.99SLIGHH86 pKa = 5.5VQIICSQPASLYY98 pKa = 10.08TEE100 pKa = 3.9NDD102 pKa = 2.91YY103 pKa = 11.4KK104 pKa = 11.21LRR106 pKa = 11.84VLNAEE111 pKa = 3.34IDD113 pKa = 4.15YY114 pKa = 11.24VKK116 pKa = 10.57EE117 pKa = 4.12LYY119 pKa = 9.64IQNSGGLQSAIDD131 pKa = 4.04DD132 pKa = 4.27LKK134 pKa = 11.44VKK136 pKa = 10.56YY137 pKa = 10.27SNCSRR142 pKa = 11.84LLDD145 pKa = 3.84NATKK149 pKa = 10.52SLYY152 pKa = 8.7RR153 pKa = 11.84TNADD157 pKa = 3.61LNNLNVSQKK166 pKa = 10.78NSDD169 pKa = 3.65EE170 pKa = 4.14LTTQITTLQAEE181 pKa = 4.5AVNLKK186 pKa = 10.38NVISQMQTEE195 pKa = 4.57RR196 pKa = 11.84DD197 pKa = 3.47QMLKK201 pKa = 10.74SMQEE205 pKa = 3.43MSEE208 pKa = 4.1INEE211 pKa = 3.84SGQRR215 pKa = 11.84QKK217 pKa = 10.89ILRR220 pKa = 11.84SEE222 pKa = 3.88EE223 pKa = 3.77AQAKK227 pKa = 6.94EE228 pKa = 3.87RR229 pKa = 11.84RR230 pKa = 11.84DD231 pKa = 3.18IGILLRR237 pKa = 11.84VINPEE242 pKa = 4.11STSSDD247 pKa = 2.85IDD249 pKa = 3.46EE250 pKa = 4.9MLTQIHH256 pKa = 7.09EE257 pKa = 4.33YY258 pKa = 10.48LISTNTLRR266 pKa = 11.84NTIEE270 pKa = 4.0KK271 pKa = 10.45LEE273 pKa = 4.34RR274 pKa = 11.84DD275 pKa = 3.7LTVSSNKK282 pKa = 9.88YY283 pKa = 10.11KK284 pKa = 9.66EE285 pKa = 4.22TSDD288 pKa = 5.01RR289 pKa = 11.84LIDD292 pKa = 4.15CANALDD298 pKa = 4.0KK299 pKa = 11.84NEE301 pKa = 4.18TEE303 pKa = 4.11KK304 pKa = 11.31SKK306 pKa = 10.97LQFNFEE312 pKa = 4.28GKK314 pKa = 9.65IKK316 pKa = 10.5SLRR319 pKa = 11.84DD320 pKa = 3.32EE321 pKa = 4.26NAKK324 pKa = 10.87LMVTNSNAQAANDD337 pKa = 4.44DD338 pKa = 3.71LTSIYY343 pKa = 10.43EE344 pKa = 4.21RR345 pKa = 11.84TSSRR349 pKa = 11.84IKK351 pKa = 10.66DD352 pKa = 3.34IIDD355 pKa = 3.3NSKK358 pKa = 10.05PLSTDD363 pKa = 3.12DD364 pKa = 3.71VNSKK368 pKa = 10.15FLHH371 pKa = 6.15NLLQVLYY378 pKa = 10.24NEE380 pKa = 4.4NLSFVVPSDD389 pKa = 3.19ILIYY393 pKa = 9.57PQNPTANDD401 pKa = 3.16NDD403 pKa = 4.21AEE405 pKa = 4.41LVTIIDD411 pKa = 3.75SDD413 pKa = 4.12NNDD416 pKa = 3.16GDD418 pKa = 5.04GGIEE422 pKa = 5.01DD423 pKa = 5.22DD424 pKa = 4.16NNVDD428 pKa = 4.28NEE430 pKa = 4.18VEE432 pKa = 4.49YY433 pKa = 11.12YY434 pKa = 10.57SSQIPRR440 pKa = 11.84TPGSNIKK447 pKa = 10.68DD448 pKa = 3.69LDD450 pKa = 3.51QLISINSPEE459 pKa = 4.18TPPLVSASSSPVNAIDD475 pKa = 4.68DD476 pKa = 3.81SSIYY480 pKa = 10.44LISRR484 pKa = 11.84DD485 pKa = 4.11LPPTPTIPILDD496 pKa = 4.11SPTISTSNSPMDD508 pKa = 3.35EE509 pKa = 4.43SYY511 pKa = 10.48TRR513 pKa = 11.84RR514 pKa = 11.84PLKK517 pKa = 10.57RR518 pKa = 11.84RR519 pKa = 11.84TSDD522 pKa = 2.8INEE525 pKa = 4.2YY526 pKa = 10.7EE527 pKa = 4.18SVARR531 pKa = 11.84KK532 pKa = 9.63RR533 pKa = 11.84NQNEE537 pKa = 4.09TVSEE541 pKa = 4.36VTTEE545 pKa = 4.27VIDD548 pKa = 3.93EE549 pKa = 4.95LIITPQSNVVNRR561 pKa = 11.84DD562 pKa = 3.12DD563 pKa = 6.18DD564 pKa = 4.71DD565 pKa = 4.71FDD567 pKa = 6.35NIITINNDD575 pKa = 3.11DD576 pKa = 4.99DD577 pKa = 6.11SNLSNVNNVNN587 pKa = 3.16

Molecular weight: 65.93 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0F7KLN5|A0A0F7KLN5_9VIRU ACH96169.1 GrBNV gp93-like protein OS=Kallithea virus OX=1654582 PE=4 SV=1
MM1 pKa = 7.2NVSIGNISEE10 pKa = 4.34FVNSIHH16 pKa = 8.01DD17 pKa = 4.43YY18 pKa = 10.43ILEE21 pKa = 4.23EE22 pKa = 4.3YY23 pKa = 10.24EE24 pKa = 3.93IIPRR28 pKa = 11.84DD29 pKa = 3.83FEE31 pKa = 4.4TKK33 pKa = 10.45LVIGGTAIAYY43 pKa = 7.83DD44 pKa = 4.08FNRR47 pKa = 11.84EE48 pKa = 3.66RR49 pKa = 11.84LIDD52 pKa = 4.09FDD54 pKa = 4.25CSKK57 pKa = 10.73LNKK60 pKa = 9.7HH61 pKa = 5.66IHH63 pKa = 4.44THH65 pKa = 6.11NDD67 pKa = 2.86VSHH70 pKa = 7.39DD71 pKa = 3.95KK72 pKa = 11.4YY73 pKa = 11.17LVCCSEE79 pKa = 4.31LDD81 pKa = 3.92IIALRR86 pKa = 11.84YY87 pKa = 10.01LNSCIFGPLKK97 pKa = 10.55VQIISTLHH105 pKa = 5.53HH106 pKa = 6.82WLPAATYY113 pKa = 7.83QHH115 pKa = 5.96VKK117 pKa = 10.63VSIQQRR123 pKa = 11.84GTLTINSYY131 pKa = 10.69FEE133 pKa = 4.03PSYY136 pKa = 11.09LFYY139 pKa = 11.14ALEE142 pKa = 4.01QSIGAHH148 pKa = 4.47IVYY151 pKa = 10.4NYY153 pKa = 8.55FHH155 pKa = 7.15SNIVTYY161 pKa = 10.83NSYY164 pKa = 10.99NLQGKK169 pKa = 7.87LTTIMVNARR178 pKa = 11.84RR179 pKa = 11.84RR180 pKa = 11.84SRR182 pKa = 11.84SRR184 pKa = 11.84SRR186 pKa = 11.84SASPARR192 pKa = 11.84RR193 pKa = 11.84SSRR196 pKa = 11.84ARR198 pKa = 11.84SPSPAGSKK206 pKa = 9.46RR207 pKa = 11.84RR208 pKa = 11.84RR209 pKa = 11.84PGRR212 pKa = 11.84PRR214 pKa = 11.84RR215 pKa = 11.84SSRR218 pKa = 11.84SGPRR222 pKa = 11.84PVAVARR228 pKa = 11.84RR229 pKa = 11.84RR230 pKa = 11.84SRR232 pKa = 11.84SRR234 pKa = 11.84SRR236 pKa = 11.84SRR238 pKa = 11.84SRR240 pKa = 11.84SRR242 pKa = 11.84AA243 pKa = 3.25

Molecular weight: 27.97 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

95

0

95

37704

53

1396

396.9

45.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.161 ± 0.159

1.841 ± 0.113

6.233 ± 0.254

4.835 ± 0.164

4.042 ± 0.159

3.451 ± 0.143

2.183 ± 0.109

8.718 ± 0.206

6.296 ± 0.231

8.031 ± 0.216

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.607 ± 0.104

8.649 ± 0.22

4.411 ± 0.203

4.339 ± 0.27

4.381 ± 0.144

8.272 ± 0.233

6.981 ± 0.326

5.339 ± 0.142

0.565 ± 0.044

4.665 ± 0.221
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski