bacterium HR38

Taxonomy: cellular organisms; Bacteria; unclassified Bacteria

Average proteome isoelectric point is 6.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 485 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H6AQR4|A0A2H6AQR4_9BACT Antitoxin VapB41 OS=bacterium HR38 OX=2035433 GN=HRbin38_00073 PE=4 SV=1
MM1 pKa = 8.22DD2 pKa = 4.5YY3 pKa = 11.38SEE5 pKa = 4.93MPYY8 pKa = 10.3QEE10 pKa = 4.3ARR12 pKa = 11.84KK13 pKa = 8.94QAVKK17 pKa = 10.7VLEE20 pKa = 4.81DD21 pKa = 4.13GYY23 pKa = 11.53GDD25 pKa = 4.38AVILKK30 pKa = 9.4DD31 pKa = 3.56AHH33 pKa = 6.71GYY35 pKa = 6.16WALYY39 pKa = 8.94YY40 pKa = 10.52LYY42 pKa = 9.88GFQVPPPEE50 pKa = 5.1APPHH54 pKa = 5.16WMEE57 pKa = 4.33GPFPGEE63 pKa = 3.53EE64 pKa = 4.79GIRR67 pKa = 11.84SPYY70 pKa = 10.42EE71 pKa = 3.46MQKK74 pKa = 10.54FLEE77 pKa = 4.31EE78 pKa = 4.15QGDD81 pKa = 3.86FTYY84 pKa = 11.16LNDD87 pKa = 3.21VDD89 pKa = 4.15

Molecular weight: 10.36 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H6ARN6|A0A2H6ARN6_9BACT Ribose import ATP-binding protein RbsA OS=bacterium HR38 OX=2035433 GN=rbsA PE=4 SV=1
MM1 pKa = 7.67ARR3 pKa = 11.84RR4 pKa = 11.84ALWGSGPTTFCTSMRR19 pKa = 11.84FSVRR23 pKa = 11.84VPVLSVAITVTLPKK37 pKa = 10.45ASTAARR43 pKa = 11.84RR44 pKa = 11.84RR45 pKa = 11.84TTALRR50 pKa = 11.84RR51 pKa = 11.84AMRR54 pKa = 11.84STPRR58 pKa = 11.84AKK60 pKa = 10.61ARR62 pKa = 11.84VSTAGSPSGTAATARR77 pKa = 11.84ATAKK81 pKa = 10.07RR82 pKa = 11.84RR83 pKa = 11.84TWAEE87 pKa = 3.76KK88 pKa = 9.7PSARR92 pKa = 11.84KK93 pKa = 9.09PSATRR98 pKa = 11.84AAASPITQRR107 pKa = 11.84ATLWPKK113 pKa = 9.1PSRR116 pKa = 11.84RR117 pKa = 11.84RR118 pKa = 11.84SRR120 pKa = 11.84GVFSSRR126 pKa = 11.84IPAKK130 pKa = 10.21SPAMRR135 pKa = 11.84PMALLRR141 pKa = 11.84PVRR144 pKa = 11.84LTSIRR149 pKa = 11.84ALPRR153 pKa = 11.84TNRR156 pKa = 11.84VPEE159 pKa = 4.28KK160 pKa = 9.77TSSPTLFSTGTLSPVRR176 pKa = 11.84RR177 pKa = 11.84LSSTKK182 pKa = 9.72TPLASRR188 pKa = 11.84STPSAGMRR196 pKa = 11.84SPASRR201 pKa = 11.84RR202 pKa = 11.84TTSPGTISRR211 pKa = 11.84LGRR214 pKa = 11.84CWKK217 pKa = 10.01RR218 pKa = 11.84PSRR221 pKa = 11.84RR222 pKa = 11.84TQAMGSARR230 pKa = 11.84ALRR233 pKa = 11.84RR234 pKa = 11.84LRR236 pKa = 11.84ASSEE240 pKa = 3.99RR241 pKa = 11.84FSCRR245 pKa = 11.84VPRR248 pKa = 11.84VALRR252 pKa = 11.84IRR254 pKa = 11.84MARR257 pKa = 11.84MAKK260 pKa = 10.21ASRR263 pKa = 11.84GSPWAPSS270 pKa = 3.14

Molecular weight: 29.45 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

485

0

485

126724

34

1061

261.3

28.78

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.603 ± 0.123

0.485 ± 0.036

3.507 ± 0.068

8.219 ± 0.14

3.946 ± 0.072

9.546 ± 0.138

2.053 ± 0.061

2.942 ± 0.093

3.658 ± 0.083

14.853 ± 0.215

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.663 ± 0.039

1.605 ± 0.06

6.474 ± 0.101

3.051 ± 0.082

7.752 ± 0.126

3.89 ± 0.07

3.786 ± 0.08

7.662 ± 0.114

1.478 ± 0.059

2.828 ± 0.058
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski