Prevotella oryzae DSM 17970

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Bacteroidia; Bacteroidales; Prevotellaceae; Prevotella; Prevotella oryzae

Average proteome isoelectric point is 6.75

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 244 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A011A6W0|A0A011A6W0_9BACT Uncharacterized protein OS=Prevotella oryzae DSM 17970 OX=915438 GN=XylorDRAFT_0106 PE=4 SV=1
MM1 pKa = 8.41DD2 pKa = 4.09DD3 pKa = 3.51TKK5 pKa = 11.66NLVKK9 pKa = 10.15TITKK13 pKa = 9.91GIQEE17 pKa = 4.22KK18 pKa = 10.15KK19 pKa = 9.61GCNIVIADD27 pKa = 4.38LTQIEE32 pKa = 4.39DD33 pKa = 4.1TICRR37 pKa = 11.84YY38 pKa = 9.75LVICQGNSSSQVEE51 pKa = 4.88AITEE55 pKa = 4.28SVGDD59 pKa = 3.57YY60 pKa = 10.99VRR62 pKa = 11.84INSGEE67 pKa = 4.12KK68 pKa = 7.38PTHH71 pKa = 4.95VVGLGSSQWVAMDD84 pKa = 3.6YY85 pKa = 11.24SDD87 pKa = 4.39VLVHH91 pKa = 6.59VFLPDD96 pKa = 3.04TRR98 pKa = 11.84EE99 pKa = 4.06FYY101 pKa = 10.95DD102 pKa = 5.95LEE104 pKa = 4.46HH105 pKa = 6.68LWADD109 pKa = 3.41AKK111 pKa = 9.87LTKK114 pKa = 10.07IPNLDD119 pKa = 3.22

Molecular weight: 13.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A010ZKQ7|A0A010ZKQ7_9BACT Endopolygalacturonase OS=Prevotella oryzae DSM 17970 OX=915438 GN=XylorDRAFT_0169 PE=3 SV=1
MM1 pKa = 7.52LSYY4 pKa = 10.86YY5 pKa = 9.02VANVINFKK13 pKa = 10.78NIARR17 pKa = 11.84RR18 pKa = 11.84MYY20 pKa = 11.14EE21 pKa = 3.79MFHH24 pKa = 6.25TNVFFDD30 pKa = 3.27NKK32 pKa = 10.78KK33 pKa = 10.46NINRR37 pKa = 3.88

Molecular weight: 4.6 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

244

0

244

100610

32

1364

412.3

46.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.2 ± 0.144

1.268 ± 0.05

5.998 ± 0.093

5.235 ± 0.149

4.641 ± 0.092

6.809 ± 0.148

1.958 ± 0.06

7.961 ± 0.167

7.233 ± 0.13

8.331 ± 0.128

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.865 ± 0.065

6.364 ± 0.129

3.354 ± 0.079

3.192 ± 0.084

3.988 ± 0.077

6.951 ± 0.128

5.619 ± 0.114

5.941 ± 0.113

1.456 ± 0.066

4.637 ± 0.121
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski