Lysobacter sp. TY2-98

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Xanthomonadales; Xanthomonadaceae; Lysobacter; unclassified Lysobacter

Average proteome isoelectric point is 6.66

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2919 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A345ZMC4|A0A345ZMC4_9GAMM Uncharacterized protein OS=Lysobacter sp. TY2-98 OX=2290922 GN=DWG18_11845 PE=4 SV=1
MM1 pKa = 7.31KK2 pKa = 10.53NVVRR6 pKa = 11.84LAILASVLLPALAFADD22 pKa = 4.22EE23 pKa = 3.86RR24 pKa = 11.84WYY26 pKa = 9.7WATAQCSDD34 pKa = 3.89EE35 pKa = 5.12DD36 pKa = 4.44DD37 pKa = 3.55EE38 pKa = 4.73TVYY41 pKa = 10.47MFAALVDD48 pKa = 3.67ITTDD52 pKa = 3.01IDD54 pKa = 3.94PDD56 pKa = 3.57SDD58 pKa = 3.69EE59 pKa = 4.32PQEE62 pKa = 4.59SKK64 pKa = 11.14DD65 pKa = 3.26HH66 pKa = 6.25ARR68 pKa = 11.84FGFGNYY74 pKa = 10.16AEE76 pKa = 4.39DD77 pKa = 4.15TYY79 pKa = 11.73AEE81 pKa = 4.45QYY83 pKa = 10.66CGVAEE88 pKa = 3.91ITAQNMYY95 pKa = 9.03ATSSFDD101 pKa = 3.34TRR103 pKa = 11.84EE104 pKa = 3.86EE105 pKa = 4.29AEE107 pKa = 4.51ASLDD111 pKa = 3.65DD112 pKa = 3.79YY113 pKa = 11.17VARR116 pKa = 11.84EE117 pKa = 4.14EE118 pKa = 4.75ADD120 pKa = 3.3DD121 pKa = 4.58RR122 pKa = 11.84PWLKK126 pKa = 10.17IIRR129 pKa = 11.84VDD131 pKa = 3.41EE132 pKa = 3.99YY133 pKa = 11.29DD134 pKa = 3.61YY135 pKa = 11.7DD136 pKa = 3.86STEE139 pKa = 3.57

Molecular weight: 15.87 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A345ZHZ0|A0A345ZHZ0_9GAMM GGDEF domain-containing protein OS=Lysobacter sp. TY2-98 OX=2290922 GN=DWG18_03035 PE=4 SV=1
MM1 pKa = 7.05ATKK4 pKa = 10.52KK5 pKa = 9.28ATKK8 pKa = 10.2KK9 pKa = 9.24STAKK13 pKa = 10.58KK14 pKa = 8.39ATKK17 pKa = 9.87KK18 pKa = 10.55AAGKK22 pKa = 10.04KK23 pKa = 9.88AGAKK27 pKa = 9.33KK28 pKa = 10.18AVRR31 pKa = 11.84KK32 pKa = 6.31TAKK35 pKa = 9.84KK36 pKa = 10.23ASAKK40 pKa = 9.87KK41 pKa = 10.15AVRR44 pKa = 11.84KK45 pKa = 6.36TAKK48 pKa = 10.02KK49 pKa = 9.88AGARR53 pKa = 11.84KK54 pKa = 6.23TTAKK58 pKa = 10.48KK59 pKa = 10.45AGAKK63 pKa = 9.57KK64 pKa = 10.17AVRR67 pKa = 11.84KK68 pKa = 6.28TAKK71 pKa = 10.13KK72 pKa = 10.22AGAKK76 pKa = 9.57KK77 pKa = 10.17AVRR80 pKa = 11.84KK81 pKa = 6.28TAKK84 pKa = 10.02KK85 pKa = 9.88AGARR89 pKa = 11.84KK90 pKa = 6.23TTAKK94 pKa = 10.48KK95 pKa = 10.45AGAKK99 pKa = 9.57KK100 pKa = 10.17AVRR103 pKa = 11.84KK104 pKa = 6.28TAKK107 pKa = 10.02KK108 pKa = 9.88AGARR112 pKa = 11.84KK113 pKa = 6.23TTAKK117 pKa = 10.48KK118 pKa = 10.55AGAKK122 pKa = 9.56KK123 pKa = 10.34AGARR127 pKa = 11.84KK128 pKa = 6.82TAKK131 pKa = 10.17KK132 pKa = 10.49AGAKK136 pKa = 8.26KK137 pKa = 7.77TAKK140 pKa = 10.17KK141 pKa = 10.45AGARR145 pKa = 11.84KK146 pKa = 7.88ATRR149 pKa = 11.84RR150 pKa = 11.84RR151 pKa = 11.84ASAPAAMPPTPAPMII166 pKa = 4.32

Molecular weight: 16.95 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2919

0

2919

942600

41

3526

322.9

35.04

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.237 ± 0.067

0.797 ± 0.015

6.385 ± 0.039

5.165 ± 0.042

3.334 ± 0.025

8.389 ± 0.05

2.29 ± 0.024

4.155 ± 0.03

2.679 ± 0.039

10.262 ± 0.05

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.262 ± 0.022

2.404 ± 0.032

5.348 ± 0.033

3.291 ± 0.025

8.062 ± 0.051

5.103 ± 0.032

5.313 ± 0.043

7.862 ± 0.036

1.444 ± 0.02

2.219 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski