Rhodobacteraceae bacterium CCMM004

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodobacterales; Rhodobacteraceae; unclassified Rhodobacteraceae

Average proteome isoelectric point is 6.25

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4132 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S2XQD5|A0A3S2XQD5_9RHOB Hemin uptake protein HemP OS=Rhodobacteraceae bacterium CCMM004 OX=2499834 GN=hemP PE=4 SV=1
MM1 pKa = 7.4KK2 pKa = 9.19HH3 pKa = 5.76TNLILAAIVVAAPLAAHH20 pKa = 7.48ADD22 pKa = 3.78PLAGFDD28 pKa = 3.63VAEE31 pKa = 4.4DD32 pKa = 3.79LSRR35 pKa = 11.84FVYY38 pKa = 10.8ANAPVFDD45 pKa = 5.77DD46 pKa = 4.95GMPAYY51 pKa = 10.26GNAFVTQGYY60 pKa = 9.41IYY62 pKa = 10.03PAGTLDD68 pKa = 3.76GGVEE72 pKa = 4.1GTLANGDD79 pKa = 3.88PAFPDD84 pKa = 3.56LVIGEE89 pKa = 4.45WTCDD93 pKa = 3.24GYY95 pKa = 11.43FVADD99 pKa = 3.97GMRR102 pKa = 11.84TEE104 pKa = 4.24TGAMVITRR112 pKa = 11.84QIYY115 pKa = 9.93RR116 pKa = 11.84FDD118 pKa = 3.83NGDD121 pKa = 3.78LLISHH126 pKa = 6.9GPEE129 pKa = 3.65LADD132 pKa = 3.8PGVSVTRR139 pKa = 11.84AVTGGTGDD147 pKa = 3.63YY148 pKa = 11.09AEE150 pKa = 5.02APAEE154 pKa = 4.03IEE156 pKa = 4.06QVLLGMSDD164 pKa = 3.67GYY166 pKa = 10.6GVRR169 pKa = 11.84LQIEE173 pKa = 4.54LTSDD177 pKa = 3.38SASS180 pKa = 3.19

Molecular weight: 18.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S2UH17|A0A3S2UH17_9RHOB Beta_helix domain-containing protein OS=Rhodobacteraceae bacterium CCMM004 OX=2499834 GN=DXV76_08310 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84KK13 pKa = 9.18RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.37AGRR28 pKa = 11.84KK29 pKa = 8.54ILNARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.07SLSAA44 pKa = 3.93

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4132

0

4132

1310337

23

2728

317.1

34.12

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.3 ± 0.073

0.87 ± 0.011

6.413 ± 0.04

5.498 ± 0.038

3.424 ± 0.023

9.137 ± 0.044

2.061 ± 0.018

4.415 ± 0.029

2.142 ± 0.028

10.102 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.525 ± 0.018

2.004 ± 0.022

5.638 ± 0.035

2.695 ± 0.021

7.773 ± 0.047

4.365 ± 0.024

5.38 ± 0.023

7.64 ± 0.033

1.514 ± 0.016

2.106 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski