Proteome-pI 2.0 - Proteome Isoelectric Point Database

Bifidobacterium longum (strain NCC 2705)

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Bifidobacteriales; Bifidobacteriaceae; Bifidobacterium; Bifidobacterium longum

Average proteome isoelectric point is 6.07

Get precalculated fractions of proteins

Acidic

pI < 6.8

6.8-7.4

pI > 7.4

Basic

All

Virtual 2D-PAGE plot for 1725 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions

Protein with the lowest isoelectric point:

>sp|Q8G4H3|FTSK_BIFLO DNA translocase FtsK OS=Bifidobacterium longum (strain NCC 2705) OX=206672 GN=ftsK PE=3 SV=1

MM1 pKa = 7.53YY2 pKa = 10.64PMGEE6 pKa = 4.19YY7 pKa = 10.66GADD10 pKa = 3.21RR11 pKa = 11.84AIYY14 pKa = 9.89IDD16 pKa = 5.59LRR18 pKa = 11.84TKK20 pKa = 10.83DD21 pKa = 3.91DD22 pKa = 4.04EE23 pKa = 4.46RR24 pKa = 11.84TMMAQDD30 pKa = 3.51YY31 pKa = 9.8DD32 pKa = 3.8SPRR35 pKa = 11.84NKK37 pKa = 10.84DD38 pKa = 3.11EE39 pKa = 5.7DD40 pKa = 4.16EE41 pKa = 4.93EE42 pKa = 4.45SLQALGKK49 pKa = 9.93SSQNASSDD57 pKa = 3.15IDD59 pKa = 3.91DD60 pKa = 5.1DD61 pKa = 4.41EE62 pKa = 4.37NAIAEE67 pKa = 4.35DD68 pKa = 4.23YY69 pKa = 9.11EE70 pKa = 5.05LPGADD75 pKa = 4.92LSNEE79 pKa = 4.01DD80 pKa = 3.61SSVTVIPMQGDD91 pKa = 3.58EE92 pKa = 5.57FICSQCFLVKK102 pKa = 10.28HH103 pKa = 6.44RR104 pKa = 11.84SQLAYY109 pKa = 9.35TDD111 pKa = 4.23EE112 pKa = 5.36DD113 pKa = 3.9GQPVCEE119 pKa = 4.12EE120 pKa = 4.24CAAA123 pKa = 3.98

Molecular weight: 13.73 kDa
Isoelectric point according different methods:

Protein with the highest isoelectric point:

>tr|Q8G6K3|Q8G6K3_BIFLO DNA replication and repair protein RecF OS=Bifidobacterium longum (strain NCC 2705) OX=206672 GN=recF PE=3 SV=1

MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84RR11 pKa = 11.84RR12 pKa = 11.84HH13 pKa = 4.82MKK15 pKa = 9.64HH16 pKa = 5.7GFRR19 pKa = 11.84LRR21 pKa = 11.84MRR23 pKa = 11.84TRR25 pKa = 11.84SGRR28 pKa = 11.84AVINRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.08GRR39 pKa = 11.84KK40 pKa = 6.41TLSAA44 pKa = 4.15

Molecular weight: 5.39 kDa
Isoelectric point according different methods:

Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try ESI

ChTry ESI

ArgC ESI

LysN ESI

TryLysC ESI

Try MALDI

ChTry MALDI

ArgC MALDI

LysN MALDI

TryLysC MALDI

Try LTQ

ChTry LTQ

ArgC LTQ

LysN LTQ

TryLysC LTQ

Try MSlow

ChTry MSlow

ArgC MSlow

LysN MSlow

TryLysC MSlow

Try MShigh

ChTry MShigh

ArgC MShigh

LysN MShigh

TryLysC MShigh

General Statistics

Number of major isoforms	Number of additional isoforms	Number of all proteins	Number of amino acids	Min. Seq. Length	Max. Seq. Length	Avg. Seq. Length	Avg. Mol. Weight
1725	0	1725	638887	15	3172	370.4	40.31

Amino acid frequency

Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu
11.152 ± 0.083	0.938 ± 0.022	6.519 ± 0.056	5.661 ± 0.063	3.415 ± 0.035	8.046 ± 0.055	2.189 ± 0.03	5.374 ± 0.045	4.063 ± 0.048	8.667 ± 0.047

Met	Asn	Gln	Pro	Arg	Ser	Thr	Val	Trp	Tyr
2.563 ± 0.03	3.435 ± 0.04	4.71 ± 0.042	3.412 ± 0.033	5.735 ± 0.063	6.128 ± 0.047	6.253 ± 0.062	7.708 ± 0.043	1.354 ± 0.024	2.679 ± 0.033

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here

See this proteome in:

Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski