Lactococcus phage 5171F

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Ceduovirus; Lactococcus virus 5171F

Average proteome isoelectric point is 6.19

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 37 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A650EQW5|A0A650EQW5_9CAUD Uncharacterized protein OS=Lactococcus phage 5171F OX=2675235 GN=5171F_000001 PE=4 SV=1
MM1 pKa = 7.9INLQNKK7 pKa = 9.34KK8 pKa = 10.5LDD10 pKa = 3.35IKK12 pKa = 11.04EE13 pKa = 4.07FLQEE17 pKa = 3.86LGFTVSLDD25 pKa = 3.68YY26 pKa = 10.86EE27 pKa = 4.28RR28 pKa = 11.84EE29 pKa = 3.94PMGVMFAEE37 pKa = 4.18IHH39 pKa = 5.97PVVSQVSNNSAIYY52 pKa = 10.02QSFRR56 pKa = 11.84TLEE59 pKa = 3.92VEE61 pKa = 4.3LMVICTEE68 pKa = 3.87EE69 pKa = 4.47TEE71 pKa = 4.2NSLYY75 pKa = 10.35MAVQLLSDD83 pKa = 3.19EE84 pKa = 4.62HH85 pKa = 8.26YY86 pKa = 10.52IYY88 pKa = 11.28ANTITDD94 pKa = 3.26NTNIIKK100 pKa = 10.59LRR102 pKa = 11.84GNYY105 pKa = 9.44YY106 pKa = 10.54DD107 pKa = 3.54

Molecular weight: 12.45 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A650F6N6|A0A650F6N6_9CAUD Uncharacterized protein OS=Lactococcus phage 5171F OX=2675235 GN=5171F_000009 PE=4 SV=1
MM1 pKa = 7.41ATTNKK6 pKa = 8.11VTGLEE11 pKa = 4.14KK12 pKa = 10.14FTEE15 pKa = 4.18KK16 pKa = 10.32QLKK19 pKa = 9.46KK20 pKa = 10.55VWLEE24 pKa = 3.86MVDD27 pKa = 4.27SFNSNQNTVKK37 pKa = 10.51RR38 pKa = 11.84SYY40 pKa = 10.61KK41 pKa = 10.29SSLGGDD47 pKa = 4.01FSRR50 pKa = 11.84YY51 pKa = 7.98PVKK54 pKa = 10.63FDD56 pKa = 3.19TKK58 pKa = 10.65KK59 pKa = 8.73ITKK62 pKa = 9.39QVTRR66 pKa = 11.84SYY68 pKa = 11.74GSLKK72 pKa = 10.3SGNIGVVNGFKK83 pKa = 10.86AKK85 pKa = 10.41DD86 pKa = 3.84EE87 pKa = 4.14SWKK90 pKa = 9.36MLNVLLHH97 pKa = 6.7DD98 pKa = 4.66RR99 pKa = 11.84NLHH102 pKa = 4.29QRR104 pKa = 11.84YY105 pKa = 8.52GQTLVRR111 pKa = 11.84ATHH114 pKa = 6.09EE115 pKa = 4.31MDD117 pKa = 5.69DD118 pKa = 3.36KK119 pKa = 11.12TKK121 pKa = 10.59NIKK124 pKa = 10.07RR125 pKa = 11.84KK126 pKa = 9.53LRR128 pKa = 11.84SITNNGG134 pKa = 2.99

Molecular weight: 15.43 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

37

0

37

6210

31

706

167.8

19.09

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.119 ± 0.586

0.853 ± 0.185

6.039 ± 0.318

7.407 ± 0.505

4.638 ± 0.295

6.973 ± 0.544

1.24 ± 0.192

6.425 ± 0.463

9.05 ± 0.481

8.341 ± 0.407

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.915 ± 0.313

6.57 ± 0.338

2.528 ± 0.352

3.961 ± 0.364

3.494 ± 0.254

5.411 ± 0.4

6.345 ± 0.495

5.797 ± 0.368

1.433 ± 0.169

4.444 ± 0.447
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski