Methylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Betaproteobacteria; Nitrosomonadales; Methylophilaceae; Methylobacillus; Methylobacillus flagellatus

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2609 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q1H0L6|Q1H0L6_METFK 3-isopropylmalate dehydrogenase OS=Methylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875) OX=265072 GN=leuB PE=3 SV=1
MM1 pKa = 7.34NMTSVRR7 pKa = 11.84AIIGASLLAVASLAQANATHH27 pKa = 6.75EE28 pKa = 4.31SFIDD32 pKa = 3.6VAGSVSFDD40 pKa = 2.58GWNQLNRR47 pKa = 11.84NRR49 pKa = 11.84TDD51 pKa = 3.03AKK53 pKa = 10.93GNPDD57 pKa = 4.45PLTSAQLEE65 pKa = 4.33AGTAGNVAGSGDD77 pKa = 3.86ALLTLISGSYY87 pKa = 8.72YY88 pKa = 9.55PAGAGLYY95 pKa = 9.52GNASITFSDD104 pKa = 3.36NTVASNISSLAFQGIINDD122 pKa = 3.62FDD124 pKa = 4.33GSFGGTPFSLSLSYY138 pKa = 11.14NGGDD142 pKa = 3.33QAIAGTLVNFVDD154 pKa = 3.66TGTAADD160 pKa = 3.94YY161 pKa = 11.37YY162 pKa = 10.83YY163 pKa = 9.73FTWDD167 pKa = 3.48LSGISTPITSYY178 pKa = 10.19TLNFDD183 pKa = 3.24IGFSQSLAFQIDD195 pKa = 3.43QVAAVPEE202 pKa = 4.23PSTYY206 pKa = 11.5AMLMLGLGAVGFAARR221 pKa = 11.84RR222 pKa = 11.84RR223 pKa = 11.84KK224 pKa = 8.85QAA226 pKa = 2.99

Molecular weight: 23.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q1GXM6|ATPF_METFK ATP synthase subunit b OS=Methylobacillus flagellatus (strain KT / ATCC 51484 / DSM 6875) OX=265072 GN=atpF PE=3 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.25QPSKK9 pKa = 7.79TRR11 pKa = 11.84RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.79GFLVRR21 pKa = 11.84MKK23 pKa = 9.7TRR25 pKa = 11.84GGRR28 pKa = 11.84AVIAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.17GRR39 pKa = 11.84ARR41 pKa = 11.84LAVV44 pKa = 3.42

Molecular weight: 5.04 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2609

0

2609

847630

35

3332

324.9

35.79

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.445 ± 0.057

0.82 ± 0.015

5.261 ± 0.033

5.867 ± 0.044

3.708 ± 0.031

7.585 ± 0.05

2.475 ± 0.03

5.821 ± 0.037

4.162 ± 0.04

10.811 ± 0.066

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.596 ± 0.025

3.501 ± 0.037

4.602 ± 0.034

4.457 ± 0.037

5.917 ± 0.04

5.904 ± 0.036

5.003 ± 0.036

7.044 ± 0.042

1.288 ± 0.019

2.734 ± 0.028
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski