Kriegella aquimaris

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Kriegella

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4956 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1G9IA92|A0A1G9IA92_9FLAO PhnB protein OS=Kriegella aquimaris OX=192904 GN=SAMN04488514_10157 PE=4 SV=1
MM1 pKa = 7.68FEE3 pKa = 4.7TILFNPTMTYY13 pKa = 9.4TRR15 pKa = 11.84INRR18 pKa = 11.84FFPRR22 pKa = 11.84LNLDD26 pKa = 3.09FTLALRR32 pKa = 11.84FSVKK36 pKa = 10.56ALLCFGMMSLSTNWVAGQEE55 pKa = 4.26TYY57 pKa = 10.88RR58 pKa = 11.84DD59 pKa = 3.57NFSSVNYY66 pKa = 9.96GNNNGSLNFSTDD78 pKa = 2.99WIEE81 pKa = 4.53TGDD84 pKa = 3.71TDD86 pKa = 5.07GGPSSQYY93 pKa = 10.59IRR95 pKa = 11.84ISSNRR100 pKa = 11.84LEE102 pKa = 4.72LYY104 pKa = 10.03YY105 pKa = 10.58LYY107 pKa = 10.75SEE109 pKa = 4.97TIRR112 pKa = 11.84RR113 pKa = 11.84SADD116 pKa = 3.05LSGASTATLSFNWQAISLGGNRR138 pKa = 11.84SLAVQVSNNGGASYY152 pKa = 8.96ATVGTITGNNSSGTFSEE169 pKa = 5.63DD170 pKa = 2.33ISAYY174 pKa = 9.7ISATTTIRR182 pKa = 11.84FRR184 pKa = 11.84KK185 pKa = 9.98SNSNWRR191 pKa = 11.84NDD193 pKa = 3.27DD194 pKa = 3.43YY195 pKa = 12.18AFIDD199 pKa = 3.64NLQISATFPAPIPIIEE215 pKa = 4.2VDD217 pKa = 3.96DD218 pKa = 3.87VTVDD222 pKa = 3.43EE223 pKa = 5.53DD224 pKa = 3.5GGNAIFTVSHH234 pKa = 6.8TGTNAAGAFTVNYY247 pKa = 7.15QTINGSANAGSDD259 pKa = 3.55YY260 pKa = 8.09TTTTGVLNFNGNSGDD275 pKa = 3.71TEE277 pKa = 4.47TFSVPILDD285 pKa = 4.87DD286 pKa = 3.46ATIEE290 pKa = 4.06NAEE293 pKa = 4.31DD294 pKa = 3.54FTIQFLAVSDD304 pKa = 4.01PTVDD308 pKa = 3.17ITDD311 pKa = 3.76TAIGTIVDD319 pKa = 4.01DD320 pKa = 5.12DD321 pKa = 4.49ALIMIDD327 pKa = 3.72GGSATSCSDD336 pKa = 2.69IFFDD340 pKa = 5.51PGGLSNYY347 pKa = 10.05SNNEE351 pKa = 3.66DD352 pKa = 3.07VTYY355 pKa = 8.99TICPDD360 pKa = 3.37TADD363 pKa = 3.45TYY365 pKa = 11.57ININFSTFEE374 pKa = 4.18VVSGDD379 pKa = 3.2VLYY382 pKa = 10.11IYY384 pKa = 10.47EE385 pKa = 4.37GTTTGGTLIGQYY397 pKa = 10.86DD398 pKa = 3.95SANIPTAINSSHH410 pKa = 7.15ASGCLTFRR418 pKa = 11.84FTSNGDD424 pKa = 3.18TTGAGWEE431 pKa = 3.98AAVNCFPEE439 pKa = 4.96GPIIIIDD446 pKa = 4.64DD447 pKa = 3.4ISFDD451 pKa = 3.89EE452 pKa = 5.39DD453 pKa = 2.77IGNAVFTVRR462 pKa = 11.84STRR465 pKa = 11.84AAHH468 pKa = 5.3GTNVFLLGFVNQSFTVDD485 pKa = 4.1FQTVDD490 pKa = 3.19GSALAGSDD498 pKa = 3.45YY499 pKa = 7.98TTTSGTLTFTGALNNVQTISVPIANDD525 pKa = 3.75GVPEE529 pKa = 3.98FAEE532 pKa = 4.3DD533 pKa = 3.56FTIEE537 pKa = 3.96FTGANAQYY545 pKa = 9.93ATVNYY550 pKa = 8.69TDD552 pKa = 3.79TGIGTINSQILANDD566 pKa = 4.05PLTLFQEE573 pKa = 4.19FDD575 pKa = 3.71GYY577 pKa = 11.39FDD579 pKa = 3.64YY580 pKa = 8.05TTTGGSLRR588 pKa = 11.84TSGNGGDD595 pKa = 3.67PCAITTSSTNRR606 pKa = 11.84LVSPIPNTAAIEE618 pKa = 3.89RR619 pKa = 11.84AYY621 pKa = 10.76LYY623 pKa = 7.8WAHH626 pKa = 6.65SNTVRR631 pKa = 11.84DD632 pKa = 4.03DD633 pKa = 3.52NVTFEE638 pKa = 4.34GQNVSANFLYY648 pKa = 8.95QTSLTNRR655 pKa = 11.84NFYY658 pKa = 10.97GYY660 pKa = 10.55VSDD663 pKa = 3.78VTSIVAAIPNISTNDD678 pKa = 3.16FDD680 pKa = 6.83FSDD683 pKa = 4.77LDD685 pKa = 3.38IDD687 pKa = 3.99NTGSYY692 pKa = 10.49CSTATVLGGWSLIVFYY708 pKa = 10.32EE709 pKa = 4.03DD710 pKa = 3.34RR711 pKa = 11.84SLPAVNINLYY721 pKa = 10.54QGFDD725 pKa = 3.45GLSNDD730 pKa = 3.88GNSFTLDD737 pKa = 2.87SFYY740 pKa = 11.09AIAGAGAKK748 pKa = 8.67STFLSWEE755 pKa = 4.11GDD757 pKa = 3.33PDD759 pKa = 3.97LASGGANPEE768 pKa = 3.87RR769 pKa = 11.84LTITNQANTTSNLTGDD785 pKa = 4.32GGQTGTNAYY794 pKa = 10.72NSTIYY799 pKa = 10.85DD800 pKa = 3.56NTVGPVYY807 pKa = 9.69NTSNIYY813 pKa = 10.55GVDD816 pKa = 3.72LDD818 pKa = 4.53TYY820 pKa = 10.17DD821 pKa = 3.03ISSFISPGDD830 pKa = 3.67SQVTANVNVGQDD842 pKa = 3.69FVISAAVVLKK852 pKa = 10.96VPSNLIAGRR861 pKa = 11.84VFEE864 pKa = 4.59DD865 pKa = 3.22VNYY868 pKa = 10.2PGGAGRR874 pKa = 11.84DD875 pKa = 3.57QATSGGIGISGAIVEE890 pKa = 5.04LFDD893 pKa = 4.37SSGNFLQRR901 pKa = 11.84KK902 pKa = 4.49TTNITGNYY910 pKa = 8.89SFGGMADD917 pKa = 2.99GDD919 pKa = 4.26FNVKK923 pKa = 9.05VVNSTVRR930 pKa = 11.84SNRR933 pKa = 11.84NNGLNCSACYY943 pKa = 9.51PIQTFRR949 pKa = 11.84TYY951 pKa = 11.52SDD953 pKa = 2.97VDD955 pKa = 4.01SNLFEE960 pKa = 4.71VINEE964 pKa = 3.96IGGANPNATQDD975 pKa = 3.44VALGIINGAQSVSTVSVASNGVVDD999 pKa = 4.31MDD1001 pKa = 4.09FGFNFSTVVNTNDD1014 pKa = 3.34AGQGSLAQFVVNSNNLGEE1032 pKa = 4.58TILDD1036 pKa = 3.56IEE1038 pKa = 4.84PNSIFDD1044 pKa = 3.81PAAEE1048 pKa = 3.98EE1049 pKa = 4.1DD1050 pKa = 3.78VSIFMIPPTGDD1061 pKa = 3.34PLGRR1065 pKa = 11.84TADD1068 pKa = 3.74TNFSGGIFDD1077 pKa = 5.05ISISNINTITPITGTNTSIDD1097 pKa = 3.27GRR1099 pKa = 11.84TQTAYY1104 pKa = 10.74SGNTNSGTVGSGGTVVGVSANSLPNFDD1131 pKa = 3.95RR1132 pKa = 11.84PVIQVHH1138 pKa = 6.39RR1139 pKa = 11.84NDD1141 pKa = 3.62GDD1143 pKa = 3.79VLEE1146 pKa = 4.6LQGNNTLIRR1155 pKa = 11.84GLSIYY1160 pKa = 10.64ADD1162 pKa = 3.77SNAGILVSGGSSNIIGNLLGVNASGVAAGNIDD1194 pKa = 3.83YY1195 pKa = 10.67GVEE1198 pKa = 3.94IVGGTTTIDD1207 pKa = 3.03GNYY1210 pKa = 9.65ISGNTDD1216 pKa = 2.89EE1217 pKa = 5.79GVLIDD1222 pKa = 4.6GGTSVLVQNNHH1233 pKa = 4.13ITEE1236 pKa = 4.49NGNTACSDD1244 pKa = 3.79NIRR1247 pKa = 11.84IEE1249 pKa = 4.35GGSGIVIQGNLIEE1262 pKa = 4.53NAAALGIDD1270 pKa = 3.86EE1271 pKa = 5.15NGSIGDD1277 pKa = 3.82IQISDD1282 pKa = 3.57NTIVGSGQNGGDD1294 pKa = 3.32CSGLVKK1300 pKa = 10.57SAGIRR1305 pKa = 11.84LEE1307 pKa = 4.3GNNSVVNDD1315 pKa = 4.15NIITSNGGAGIVLVGGNTSGNLISQNSIYY1344 pKa = 11.21ANGTITASLGIDD1356 pKa = 3.01IDD1358 pKa = 3.8QTNQMGDD1365 pKa = 3.17GVTLNEE1371 pKa = 4.88TGDD1374 pKa = 3.83ADD1376 pKa = 3.7NGPNGVINFPIISGVYY1392 pKa = 9.43ISSTNLTIEE1401 pKa = 4.06GWSRR1405 pKa = 11.84PGATIEE1411 pKa = 5.33LFLTDD1416 pKa = 3.68ISEE1419 pKa = 4.54GSAVAGDD1426 pKa = 3.78NEE1428 pKa = 4.57LGKK1431 pKa = 7.78TTDD1434 pKa = 3.47YY1435 pKa = 11.9GEE1437 pKa = 4.48GQAYY1441 pKa = 9.39LASFVEE1447 pKa = 5.02GSGSDD1452 pKa = 3.78LLSDD1456 pKa = 3.64ATAYY1460 pKa = 10.86LDD1462 pKa = 3.49EE1463 pKa = 5.99DD1464 pKa = 4.65GNVDD1468 pKa = 3.42TTNKK1472 pKa = 9.86FKK1474 pKa = 10.3FTIPLPPGVIKK1485 pKa = 10.96GNLVTSTATISNSTSEE1501 pKa = 4.49FSPMSIIKK1509 pKa = 9.29TYY1511 pKa = 8.8TVITNRR1517 pKa = 11.84RR1518 pKa = 11.84ITYY1521 pKa = 10.01RR1522 pKa = 11.84IKK1524 pKa = 10.96KK1525 pKa = 8.93NN1526 pKa = 3.17

Molecular weight: 160.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1G9TGQ3|A0A1G9TGQ3_9FLAO Quinol:cytochrome c oxidoreductase monoheme cytochrome subunit OS=Kriegella aquimaris OX=192904 GN=SAMN04488514_10959 PE=4 SV=1
MM1 pKa = 7.55DD2 pKa = 3.64VSYY5 pKa = 11.25RR6 pKa = 11.84SMDD9 pKa = 3.04ILFLSRR15 pKa = 11.84RR16 pKa = 11.84KK17 pKa = 9.33FKK19 pKa = 11.25KK20 pKa = 8.21NTCRR24 pKa = 11.84NAIFKK29 pKa = 10.55KK30 pKa = 10.61GILLEE35 pKa = 4.14EE36 pKa = 4.34TQKK39 pKa = 10.77KK40 pKa = 6.88QHH42 pKa = 6.59RR43 pKa = 11.84LNQTMLFLTNLTNFFVNTPCRR64 pKa = 11.84WRR66 pKa = 11.84ALMLL70 pKa = 4.36

Molecular weight: 8.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4956

0

4956

1798520

39

4105

362.9

40.83

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.598 ± 0.029

0.73 ± 0.011

5.839 ± 0.025

6.534 ± 0.03

5.049 ± 0.024

6.995 ± 0.036

1.869 ± 0.018

7.322 ± 0.031

7.247 ± 0.043

9.27 ± 0.042

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.305 ± 0.015

5.814 ± 0.029

3.7 ± 0.023

3.333 ± 0.017

3.74 ± 0.021

6.475 ± 0.028

5.675 ± 0.033

6.234 ± 0.024

1.273 ± 0.015

3.998 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski