Propionibacterium phage G4

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Doucettevirus; Propionibacterium virus G4

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 69 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1D8EUA6|A0A1D8EUA6_9CAUD Uncharacterized protein OS=Propionibacterium phage G4 OX=1897537 GN=66 PE=4 SV=1
MM1 pKa = 8.03SYY3 pKa = 10.92EE4 pKa = 4.93FITSYY9 pKa = 11.06NSPNHH14 pKa = 6.3EE15 pKa = 4.24GPVVPNPASLCVVHH29 pKa = 6.45WWGAPSGQTFEE40 pKa = 4.84GTISWLCNPASQVSAHH56 pKa = 6.01AVATAGRR63 pKa = 11.84VACIVDD69 pKa = 3.41YY70 pKa = 10.72PDD72 pKa = 4.83RR73 pKa = 11.84SWANGNDD80 pKa = 3.33WANDD84 pKa = 3.11NSITVEE90 pKa = 4.05CDD92 pKa = 2.98PNNIDD97 pKa = 3.46GTIDD101 pKa = 3.54TLVEE105 pKa = 3.72WLADD109 pKa = 3.3MVRR112 pKa = 11.84QNNLTADD119 pKa = 4.1FQLKK123 pKa = 8.17GHH125 pKa = 6.95RR126 pKa = 11.84DD127 pKa = 4.23FYY129 pKa = 11.21ATEE132 pKa = 4.41CPGDD136 pKa = 3.93YY137 pKa = 10.36YY138 pKa = 10.6PRR140 pKa = 11.84LAEE143 pKa = 3.56IRR145 pKa = 11.84QRR147 pKa = 11.84VADD150 pKa = 3.91NLGGGSGQAPAPSAPAGIHH169 pKa = 6.05VDD171 pKa = 3.87LPSWTLPTGHH181 pKa = 7.1FYY183 pKa = 11.57GLVSGGEE190 pKa = 4.02DD191 pKa = 3.16SHH193 pKa = 8.0GGFYY197 pKa = 10.46EE198 pKa = 4.25GEE200 pKa = 3.94RR201 pKa = 11.84PAIRR205 pKa = 11.84QIQLWLIRR213 pKa = 11.84HH214 pKa = 6.47GYY216 pKa = 9.4AGAVPDD222 pKa = 3.59SWADD226 pKa = 3.84GIYY229 pKa = 9.46EE230 pKa = 4.25QPTADD235 pKa = 3.26AVAAFQHH242 pKa = 6.31AEE244 pKa = 4.21RR245 pKa = 11.84PNSTDD250 pKa = 2.05RR251 pKa = 11.84WGEE254 pKa = 3.76VWADD258 pKa = 3.89DD259 pKa = 4.48LATMAANNN267 pKa = 3.81

Molecular weight: 29.03 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1D8EUB2|A0A1D8EUB2_9CAUD Uncharacterized protein OS=Propionibacterium phage G4 OX=1897537 GN=60 PE=4 SV=1
MM1 pKa = 7.08VALSRR6 pKa = 11.84ASYY9 pKa = 11.56GDD11 pKa = 3.3GTQPTQRR18 pKa = 11.84SDD20 pKa = 3.57GRR22 pKa = 11.84WAASAYY28 pKa = 10.38DD29 pKa = 3.54GWQANGNRR37 pKa = 11.84RR38 pKa = 11.84RR39 pKa = 11.84RR40 pKa = 11.84WVYY43 pKa = 10.21GRR45 pKa = 11.84TQAEE49 pKa = 4.87CKK51 pKa = 10.07RR52 pKa = 11.84KK53 pKa = 9.86LRR55 pKa = 11.84DD56 pKa = 3.38LKK58 pKa = 11.11RR59 pKa = 11.84EE60 pKa = 3.81IWSDD64 pKa = 3.47TQQVNVNPRR73 pKa = 11.84EE74 pKa = 4.29TVKK77 pKa = 10.51SWTASWLDD85 pKa = 3.61EE86 pKa = 4.14YY87 pKa = 11.34RR88 pKa = 11.84SIARR92 pKa = 11.84PTTFVTDD99 pKa = 3.36EE100 pKa = 4.26SMVRR104 pKa = 11.84NWIVPAVGARR114 pKa = 11.84RR115 pKa = 11.84LSEE118 pKa = 3.79LTARR122 pKa = 11.84DD123 pKa = 3.26ASKK126 pKa = 10.5LQRR129 pKa = 11.84VCRR132 pKa = 11.84DD133 pKa = 3.13GGLSATTSHH142 pKa = 6.62YY143 pKa = 11.24AGLLLRR149 pKa = 11.84RR150 pKa = 11.84ILKK153 pKa = 9.78AARR156 pKa = 11.84ASGYY160 pKa = 10.03RR161 pKa = 11.84IPDD164 pKa = 3.24SVMLARR170 pKa = 11.84IPGIGASNRR179 pKa = 11.84IALSAIQAANLLSTANARR197 pKa = 11.84DD198 pKa = 3.64TWPEE202 pKa = 4.07LPSLPDD208 pKa = 3.44LPYY211 pKa = 11.22GSISKK216 pKa = 9.49LAPGEE221 pKa = 4.02AQKK224 pKa = 10.87RR225 pKa = 11.84EE226 pKa = 3.97QLKK229 pKa = 9.04MEE231 pKa = 4.34RR232 pKa = 11.84LEE234 pKa = 3.8WTAARR239 pKa = 11.84NTDD242 pKa = 3.43PSRR245 pKa = 11.84WAAALMQGLRR255 pKa = 11.84SGEE258 pKa = 3.94ARR260 pKa = 11.84GLTWDD265 pKa = 3.75RR266 pKa = 11.84VDD268 pKa = 4.08LARR271 pKa = 11.84GTITIDD277 pKa = 3.33RR278 pKa = 11.84QLQRR282 pKa = 11.84IKK284 pKa = 10.38PDD286 pKa = 3.11AALPPGYY293 pKa = 10.11KK294 pKa = 8.64VTRR297 pKa = 11.84LEE299 pKa = 4.43GSHH302 pKa = 6.67CLVAPKK308 pKa = 10.19SRR310 pKa = 11.84SGVRR314 pKa = 11.84RR315 pKa = 11.84VPIVPWMGQALTRR328 pKa = 11.84WRR330 pKa = 11.84DD331 pKa = 3.33IQGDD335 pKa = 3.97SSFGLVWPLPTGAPPTRR352 pKa = 11.84VHH354 pKa = 7.58DD355 pKa = 3.95LRR357 pKa = 11.84AWRR360 pKa = 11.84GLQRR364 pKa = 11.84VAGVHH369 pKa = 6.3KK370 pKa = 10.45EE371 pKa = 3.91DD372 pKa = 3.45GNLYY376 pKa = 8.76VLHH379 pKa = 6.77EE380 pKa = 4.66ARR382 pKa = 11.84HH383 pKa = 5.11STVSLLLAAGVPEE396 pKa = 4.29SVVIAIVGHH405 pKa = 6.51ASFAATEE412 pKa = 4.82HH413 pKa = 6.17YY414 pKa = 11.3AHH416 pKa = 7.19TDD418 pKa = 3.47LEE420 pKa = 4.31AARR423 pKa = 11.84AALMKK428 pKa = 10.61VQDD431 pKa = 3.76RR432 pKa = 11.84LGLEE436 pKa = 4.29LEE438 pKa = 4.52SS439 pKa = 4.04

Molecular weight: 48.69 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

69

0

69

12043

30

1364

174.5

18.86

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.779 ± 0.385

1.005 ± 0.178

6.593 ± 0.406

5.107 ± 0.425

2.558 ± 0.206

8.428 ± 0.532

1.902 ± 0.222

4.841 ± 0.395

3.28 ± 0.224

8.395 ± 0.394

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.408 ± 0.155

1.843 ± 0.193

5.597 ± 0.359

3.72 ± 0.197

7.041 ± 0.633

6.302 ± 0.317

6.967 ± 0.385

7.291 ± 0.435

2.649 ± 0.209

1.295 ± 0.16
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski