Mycoplasma neurolyticum

Taxonomy: cellular organisms; Bacteria;

Average proteome isoelectric point is 7.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 836 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A449A599|A0A449A599_9MOLU DNA polymerase III delta subunit OS=Mycoplasma neurolyticum OX=2120 GN=NCTC10166_00363 PE=4 SV=1
MM1 pKa = 7.34NVPPGNDD8 pKa = 3.12YY9 pKa = 11.6DD10 pKa = 4.12LGSVKK15 pKa = 9.92TIYY18 pKa = 10.09QLQKK22 pKa = 10.43NSSNQLIYY30 pKa = 11.04DD31 pKa = 4.31LAEE34 pKa = 4.33GASGSIVLDD43 pKa = 3.18QDD45 pKa = 3.59GALVAIFTAIDD56 pKa = 3.61DD57 pKa = 4.15QLDD60 pKa = 3.57DD61 pKa = 4.69SYY63 pKa = 12.32LLFNLVNSSDD73 pKa = 3.35FDD75 pKa = 3.91YY76 pKa = 11.47FGFDD80 pKa = 3.3KK81 pKa = 11.64NEE83 pKa = 3.86IEE85 pKa = 4.75NDD87 pKa = 3.29NSFKK91 pKa = 11.03NAVKK95 pKa = 10.57NAVLKK100 pKa = 11.02DD101 pKa = 3.37PTNYY105 pKa = 10.4EE106 pKa = 4.0IIEE109 pKa = 4.16LL110 pKa = 3.93

Molecular weight: 12.22 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A449A583|A0A449A583_9MOLU ATPase OS=Mycoplasma neurolyticum OX=2120 GN=clpC PE=4 SV=1
MM1 pKa = 7.84AKK3 pKa = 9.13TKK5 pKa = 10.6AAGSTRR11 pKa = 11.84NGRR14 pKa = 11.84DD15 pKa = 2.95SAGRR19 pKa = 11.84RR20 pKa = 11.84LGVKK24 pKa = 10.52LSDD27 pKa = 3.48GQYY30 pKa = 10.53ASAGAIILRR39 pKa = 11.84QRR41 pKa = 11.84GTKK44 pKa = 9.64IFPGLNVGRR53 pKa = 11.84GNDD56 pKa = 3.45DD57 pKa = 3.36TLYY60 pKa = 11.21SLIDD64 pKa = 3.92GYY66 pKa = 11.63VKK68 pKa = 10.49FEE70 pKa = 4.15RR71 pKa = 11.84KK72 pKa = 9.37RR73 pKa = 11.84NRR75 pKa = 11.84KK76 pKa = 8.07VASVYY81 pKa = 11.08ANEE84 pKa = 4.06NN85 pKa = 3.28

Molecular weight: 9.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

836

0

836

301871

30

6304

361.1

41.84

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.336 ± 0.074

0.349 ± 0.015

5.135 ± 0.092

7.025 ± 0.068

6.342 ± 0.085

4.0 ± 0.085

1.356 ± 0.025

10.748 ± 0.111

11.997 ± 0.1

9.376 ± 0.089

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.527 ± 0.035

8.969 ± 0.143

2.362 ± 0.043

3.05 ± 0.038

2.323 ± 0.05

6.324 ± 0.057

4.895 ± 0.079

4.846 ± 0.072

1.023 ± 0.031

4.017 ± 0.057
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski