Pseudomonas phage VCM

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Otagovirus; Pseudomonas virus VCM

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 172 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0S4KWH5|A0A0S4KWH5_9CAUD Uncharacterized protein OS=Pseudomonas phage VCM OX=1729937 GN=VCM_00176 PE=4 SV=1
MM1 pKa = 7.69ARR3 pKa = 11.84MTVDD7 pKa = 3.54TNATASPDD15 pKa = 3.89VVPGTLMYY23 pKa = 10.06WKK25 pKa = 9.88EE26 pKa = 3.97DD27 pKa = 3.19PTYY30 pKa = 11.46VVMCTGILMGDD41 pKa = 4.29EE42 pKa = 4.57FPGVALDD49 pKa = 3.43SGLYY53 pKa = 9.19GEE55 pKa = 4.37QWIIDD60 pKa = 4.09EE61 pKa = 4.42YY62 pKa = 11.71AVFQGKK68 pKa = 7.66ITLEE72 pKa = 3.96QQ73 pKa = 3.51

Molecular weight: 8.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0S4KXA4|A0A0S4KXA4_9CAUD Uncharacterized protein OS=Pseudomonas phage VCM OX=1729937 GN=VCM_00002 PE=4 SV=1
MM1 pKa = 7.34GWQEE5 pKa = 4.3RR6 pKa = 11.84TSMMSPAQEE15 pKa = 3.69RR16 pKa = 11.84EE17 pKa = 3.78FWLKK21 pKa = 10.12EE22 pKa = 3.64VEE24 pKa = 3.99SRR26 pKa = 11.84MEE28 pKa = 4.01HH29 pKa = 6.65GGLLVNRR36 pKa = 11.84RR37 pKa = 11.84HH38 pKa = 5.69MPSLDD43 pKa = 3.25RR44 pKa = 11.84TPVLRR49 pKa = 11.84KK50 pKa = 9.69LVEE53 pKa = 4.53DD54 pKa = 4.04GKK56 pKa = 10.66LLRR59 pKa = 11.84VRR61 pKa = 11.84EE62 pKa = 4.38GTWMSSHH69 pKa = 5.95VRR71 pKa = 11.84RR72 pKa = 11.84TFLRR76 pKa = 11.84LPEE79 pKa = 4.02QQAA82 pKa = 3.11

Molecular weight: 9.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

172

0

172

29171

38

787

169.6

18.92

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.793 ± 0.262

1.268 ± 0.091

6.15 ± 0.195

6.537 ± 0.249

3.802 ± 0.131

7.24 ± 0.222

1.992 ± 0.106

5.581 ± 0.182

6.27 ± 0.228

7.981 ± 0.203

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.089 ± 0.096

4.535 ± 0.181

3.627 ± 0.134

3.929 ± 0.113

4.844 ± 0.155

5.488 ± 0.216

6.578 ± 0.229

7.216 ± 0.171

1.471 ± 0.077

3.61 ± 0.158
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski