bacterium 1xD42-67

Taxonomy: cellular organisms; Bacteria; unclassified Bacteria

Average proteome isoelectric point is 6.01

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3363 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3A8Z068|A0A3A8Z068_9BACT DUF1836 domain-containing protein OS=bacterium 1xD42-67 OX=2320107 GN=D7V91_12055 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 10.3KK3 pKa = 9.76ILALTMAAAMVLSLAACGGNSDD25 pKa = 4.44KK26 pKa = 11.38GSGSSNGSSSNGGGSAGPISVYY48 pKa = 9.95TNAYY52 pKa = 7.18FAPFEE57 pKa = 4.48YY58 pKa = 10.75YY59 pKa = 10.48DD60 pKa = 3.5GTDD63 pKa = 2.92IVGVDD68 pKa = 3.86VDD70 pKa = 3.64VMDD73 pKa = 4.9RR74 pKa = 11.84VGEE77 pKa = 3.97KK78 pKa = 10.37LGRR81 pKa = 11.84KK82 pKa = 8.69VSYY85 pKa = 10.51TNVEE89 pKa = 4.0FGTIIDD95 pKa = 3.96TVSAGKK101 pKa = 9.84LADD104 pKa = 3.46VGAAGITITASRR116 pKa = 11.84QEE118 pKa = 4.03QVDD121 pKa = 3.91FSDD124 pKa = 4.48PYY126 pKa = 8.51FTSVQYY132 pKa = 10.9VIFPAGTMTADD143 pKa = 3.5GTDD146 pKa = 3.3GDD148 pKa = 3.8VSYY151 pKa = 11.11ILWDD155 pKa = 3.43SLAGKK160 pKa = 10.03KK161 pKa = 9.8IGVQADD167 pKa = 3.81TTGDD171 pKa = 3.1IYY173 pKa = 11.64VDD175 pKa = 3.65MEE177 pKa = 4.21INAADD182 pKa = 4.14GDD184 pKa = 4.32DD185 pKa = 3.85YY186 pKa = 11.97DD187 pKa = 4.39LGRR190 pKa = 11.84DD191 pKa = 3.74GVLLGSGAEE200 pKa = 3.93EE201 pKa = 3.79TRR203 pKa = 11.84YY204 pKa = 10.98DD205 pKa = 3.93SAQLAADD212 pKa = 3.64AVGAGQVDD220 pKa = 4.35VVVVDD225 pKa = 4.44QLPASYY231 pKa = 10.33IVDD234 pKa = 3.49KK235 pKa = 11.57NDD237 pKa = 3.3GFEE240 pKa = 4.18CAALYY245 pKa = 9.78YY246 pKa = 10.83AAGTDD251 pKa = 3.84GASAPFPTEE260 pKa = 3.38EE261 pKa = 4.16QYY263 pKa = 11.32AICVTKK269 pKa = 10.81GQDD272 pKa = 3.38EE273 pKa = 4.5LLDD276 pKa = 4.78AINAVLSEE284 pKa = 4.23LGEE287 pKa = 4.41DD288 pKa = 4.77GINSLVMEE296 pKa = 4.41HH297 pKa = 6.45MGLKK301 pKa = 10.1

Molecular weight: 31.21 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3A8YZX1|A0A3A8YZX1_9BACT Uncharacterized protein OS=bacterium 1xD42-67 OX=2320107 GN=D7V91_09080 PE=4 SV=1
MM1 pKa = 7.45LRR3 pKa = 11.84TYY5 pKa = 9.8QPKK8 pKa = 9.6KK9 pKa = 7.95RR10 pKa = 11.84QRR12 pKa = 11.84SKK14 pKa = 9.69EE15 pKa = 3.5HH16 pKa = 6.11GFRR19 pKa = 11.84KK20 pKa = 10.07RR21 pKa = 11.84MATRR25 pKa = 11.84NGRR28 pKa = 11.84KK29 pKa = 8.84VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.22GRR39 pKa = 11.84VRR41 pKa = 11.84LTHH44 pKa = 6.42

Molecular weight: 5.42 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3363

0

3363

1021562

24

4387

303.8

33.57

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.529 ± 0.051

1.587 ± 0.021

5.948 ± 0.036

6.879 ± 0.042

3.671 ± 0.031

8.123 ± 0.042

1.774 ± 0.02

5.568 ± 0.041

4.633 ± 0.045

10.004 ± 0.063

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.742 ± 0.024

2.902 ± 0.034

4.401 ± 0.03

3.712 ± 0.026

5.993 ± 0.055

5.612 ± 0.037

5.404 ± 0.055

6.934 ± 0.037

1.169 ± 0.016

3.415 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski