Desulfobacterium autotrophicum (strain ATCC 43914 / DSM 3382 / HRM2)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; delta/epsilon subdivisions; Deltaproteobacteria; Desulfobacterales; Desulfobacteraceae; Desulfobacterium; Desulfobacterium autotrophicum

Average proteome isoelectric point is 6.81

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4846 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C0QD89|C0QD89_DESAH Protein TonB OS=Desulfobacterium autotrophicum (strain ATCC 43914 / DSM 3382 / HRM2) OX=177437 GN=tonB PE=3 SV=1
MM1 pKa = 7.39SKK3 pKa = 9.73IEE5 pKa = 4.68RR6 pKa = 11.84ITHH9 pKa = 5.68KK10 pKa = 10.89SFMYY14 pKa = 9.12TPAIIVLLAAILAFSPLNATAMTFDD39 pKa = 3.8AGEE42 pKa = 4.05MGKK45 pKa = 8.43MEE47 pKa = 3.91VSGAIKK53 pKa = 10.41ASFGATQYY61 pKa = 11.79LDD63 pKa = 4.48FVDD66 pKa = 4.63NNSKK70 pKa = 11.06GDD72 pKa = 3.9DD73 pKa = 3.66ADD75 pKa = 4.14FDD77 pKa = 4.0ATRR80 pKa = 11.84EE81 pKa = 4.08LNLRR85 pKa = 11.84VDD87 pKa = 4.49WILNDD92 pKa = 3.42NLKK95 pKa = 10.87GVVSFQIGEE104 pKa = 4.31GSTGGYY110 pKa = 9.78FGSTDD115 pKa = 3.16AAVGGEE121 pKa = 3.99EE122 pKa = 4.92DD123 pKa = 3.87GDD125 pKa = 5.04LILEE129 pKa = 4.27LDD131 pKa = 4.15KK132 pKa = 11.39LYY134 pKa = 10.35IDD136 pKa = 3.82YY137 pKa = 7.79TTNGGVNFKK146 pKa = 10.67LGSQSFNLHH155 pKa = 6.12EE156 pKa = 4.19IAYY159 pKa = 9.17GSNIFYY165 pKa = 7.63EE166 pKa = 4.55TPPGVTMMAPLSEE179 pKa = 4.33TVSLEE184 pKa = 4.16VGWFRR189 pKa = 11.84MADD192 pKa = 3.37LMDD195 pKa = 5.09DD196 pKa = 4.35SEE198 pKa = 6.29SEE200 pKa = 4.15TDD202 pKa = 3.58DD203 pKa = 3.33QADD206 pKa = 4.34FIFAKK211 pKa = 9.82MPLSMGTVKK220 pKa = 9.17VTPWAAYY227 pKa = 10.5ANIQEE232 pKa = 4.66DD233 pKa = 4.66VIDD236 pKa = 3.8TDD238 pKa = 4.2EE239 pKa = 4.63DD240 pKa = 3.6NNYY243 pKa = 10.26YY244 pKa = 10.5KK245 pKa = 10.55YY246 pKa = 10.93AYY248 pKa = 9.78FDD250 pKa = 3.76YY251 pKa = 10.84PGLLTGANSAITNANPTDD269 pKa = 3.85NVNAYY274 pKa = 9.91YY275 pKa = 10.55LGASIQFSPNKK286 pKa = 9.53NLAVSASATYY296 pKa = 11.27GDD298 pKa = 5.1MDD300 pKa = 3.79WQTATVDD307 pKa = 3.47ASISGFFTDD316 pKa = 5.39LVVDD320 pKa = 3.71YY321 pKa = 10.56KK322 pKa = 9.77MASMTPEE329 pKa = 3.89FFVLYY334 pKa = 10.77GNGPDD339 pKa = 5.43ADD341 pKa = 4.68DD342 pKa = 4.82SDD344 pKa = 4.48IDD346 pKa = 3.88MMPVLIGGPTYY357 pKa = 9.7TSSYY361 pKa = 10.28FGGSRR366 pKa = 11.84FNDD369 pKa = 3.34NMFDD373 pKa = 4.34SYY375 pKa = 11.8DD376 pKa = 3.52STYY379 pKa = 9.48ATSMWAVGFKK389 pKa = 10.78LKK391 pKa = 10.49DD392 pKa = 3.05IRR394 pKa = 11.84TGEE397 pKa = 4.14RR398 pKa = 11.84LTHH401 pKa = 6.56EE402 pKa = 4.3FQIMYY407 pKa = 10.75AEE409 pKa = 4.52GTADD413 pKa = 4.13DD414 pKa = 5.75SIFQSPDD421 pKa = 3.74DD422 pKa = 3.53ILLNEE427 pKa = 4.75DD428 pKa = 3.39EE429 pKa = 5.33SLVEE433 pKa = 4.05VNFNSEE439 pKa = 4.19FQVMKK444 pKa = 10.21HH445 pKa = 5.5LVFATEE451 pKa = 4.28LGYY454 pKa = 8.73ITFDD458 pKa = 3.33EE459 pKa = 4.78DD460 pKa = 2.99SDD462 pKa = 4.31YY463 pKa = 11.81NEE465 pKa = 5.06EE466 pKa = 4.1INGDD470 pKa = 3.9VEE472 pKa = 4.98DD473 pKa = 4.32FWKK476 pKa = 10.29IACSIEE482 pKa = 4.07LSFF485 pKa = 5.66

Molecular weight: 53.58 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C0QJ31|C0QJ31_DESAH Fe-S oxidoreductase (Iron-sulfur cluster-binding domain protein) OS=Desulfobacterium autotrophicum (strain ATCC 43914 / DSM 3382 / HRM2) OX=177437 GN=HRM2_07070 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSRR9 pKa = 11.84IKK11 pKa = 10.19RR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.67GFRR19 pKa = 11.84KK20 pKa = 10.0RR21 pKa = 11.84MSTAAGRR28 pKa = 11.84RR29 pKa = 11.84IVNSRR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.2KK41 pKa = 9.68LTAA44 pKa = 4.27

Molecular weight: 5.25 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4846

0

4846

1634900

22

3859

337.4

37.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.026 ± 0.035

1.333 ± 0.014

5.537 ± 0.028

5.967 ± 0.033

4.625 ± 0.027

7.411 ± 0.033

2.069 ± 0.015

7.093 ± 0.028

6.0 ± 0.032

9.94 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.922 ± 0.016

3.903 ± 0.02

4.392 ± 0.023

3.449 ± 0.022

5.207 ± 0.024

5.862 ± 0.025

5.609 ± 0.027

6.814 ± 0.031

1.059 ± 0.013

2.78 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski