Bacillus sp. AFS037270

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus; unclassified Bacillus (in: Bacteria)

Average proteome isoelectric point is 6.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5980 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2C1YUP9|A0A2C1YUP9_9BACI Uncharacterized protein OS=Bacillus sp. AFS037270 OX=2033499 GN=COD92_25430 PE=4 SV=1
MM1 pKa = 7.58FNRR4 pKa = 11.84IDD6 pKa = 3.48KK7 pKa = 10.04EE8 pKa = 4.31LYY10 pKa = 9.05GNEE13 pKa = 4.0YY14 pKa = 10.49QDD16 pKa = 4.45ANLNGVRR23 pKa = 11.84QEE25 pKa = 3.88MGLPSGAEE33 pKa = 3.87AGQQAMDD40 pKa = 3.95HH41 pKa = 6.69GIDD44 pKa = 3.27NDD46 pKa = 3.82LYY48 pKa = 9.24EE49 pKa = 4.85NEE51 pKa = 4.65YY52 pKa = 10.82EE53 pKa = 5.21DD54 pKa = 5.47SDD56 pKa = 4.45FNDD59 pKa = 2.97VRR61 pKa = 11.84QQMGLPNGAEE71 pKa = 4.04AGQQAVQYY79 pKa = 10.82GIDD82 pKa = 3.39NNLYY86 pKa = 8.35EE87 pKa = 5.03NEE89 pKa = 4.16YY90 pKa = 10.17KK91 pKa = 10.75DD92 pKa = 4.35SGFSDD97 pKa = 3.56VRR99 pKa = 11.84QQMVLPTRR107 pKa = 11.84ADD109 pKa = 3.46AEE111 pKa = 4.32QQAMHH116 pKa = 6.72HH117 pKa = 6.54GIDD120 pKa = 3.29NDD122 pKa = 4.09LYY124 pKa = 9.85GNEE127 pKa = 4.44YY128 pKa = 10.34EE129 pKa = 5.9DD130 pKa = 5.04SDD132 pKa = 4.5FNDD135 pKa = 2.97VRR137 pKa = 11.84QQMGLPNGAEE147 pKa = 4.27VEE149 pKa = 4.34QQAGQDD155 pKa = 4.04GIDD158 pKa = 3.19NCLYY162 pKa = 9.17EE163 pKa = 5.32NEE165 pKa = 4.25YY166 pKa = 11.04EE167 pKa = 5.21DD168 pKa = 4.69YY169 pKa = 11.28DD170 pKa = 4.46FNSVRR175 pKa = 11.84QQMGLPSGAEE185 pKa = 3.85TGQQAMHH192 pKa = 5.82QAGYY196 pKa = 9.7QAGFQAGFQAGFQTGFQTGFQAGYY220 pKa = 7.76QTGNQTGLQTGAQAGVPTGTQVGSQTGAQMGTQDD254 pKa = 3.44GAQAGTQTEE263 pKa = 4.59TQDD266 pKa = 3.05GTQAGTQTGAQDD278 pKa = 3.77GAQVEE283 pKa = 4.52AQTEE287 pKa = 4.28QQHH290 pKa = 5.48PHH292 pKa = 6.88AGHH295 pKa = 5.84QFGPRR300 pKa = 11.84DD301 pKa = 3.53LSYY304 pKa = 10.92EE305 pKa = 3.97

Molecular weight: 33.15 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2C1YCP8|A0A2C1YCP8_9BACI Thiol-disulfide oxidoreductase OS=Bacillus sp. AFS037270 OX=2033499 GN=COD92_19595 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.42RR3 pKa = 11.84TYY5 pKa = 10.24QPNKK9 pKa = 8.16RR10 pKa = 11.84KK11 pKa = 9.54HH12 pKa = 5.99SKK14 pKa = 8.79VHH16 pKa = 5.85GFRR19 pKa = 11.84SRR21 pKa = 11.84MSSANGRR28 pKa = 11.84KK29 pKa = 8.7VLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84QKK37 pKa = 10.39GRR39 pKa = 11.84KK40 pKa = 8.52VLSAA44 pKa = 4.05

Molecular weight: 5.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5980

0

5980

1805640

15

2017

301.9

33.85

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.931 ± 0.031

0.785 ± 0.008

4.852 ± 0.021

7.009 ± 0.039

4.696 ± 0.023

7.114 ± 0.028

2.063 ± 0.015

7.984 ± 0.033

6.972 ± 0.025

9.853 ± 0.033

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.692 ± 0.015

4.64 ± 0.019

3.743 ± 0.019

3.646 ± 0.019

3.884 ± 0.021

6.069 ± 0.022

5.433 ± 0.023

6.96 ± 0.026

1.127 ± 0.013

3.546 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski