Dethiobacter alkaliphilus AHT 1

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Syntrophomonadaceae; Dethiobacter; Dethiobacter alkaliphilus

Average proteome isoelectric point is 6.16

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3162 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C0GEF8|C0GEF8_9FIRM Diguanylate cyclase and metal dependent phosphohydrolase OS=Dethiobacter alkaliphilus AHT 1 OX=555088 GN=DealDRAFT_0867 PE=4 SV=1
MM1 pKa = 7.28NMAKK5 pKa = 9.96PVSKK9 pKa = 9.91YY10 pKa = 7.29WRR12 pKa = 11.84VGLVLLVIGLLAIGTALGSVPPCEE36 pKa = 4.74GPLVDD41 pKa = 3.48PVEE44 pKa = 4.36YY45 pKa = 10.62NGNPEE50 pKa = 4.31EE51 pKa = 4.35IGTRR55 pKa = 11.84INDD58 pKa = 3.94PGSGYY63 pKa = 9.99IDD65 pKa = 3.52VEE67 pKa = 4.57FDD69 pKa = 3.67GVWYY73 pKa = 10.2KK74 pKa = 10.79VFYY77 pKa = 10.24EE78 pKa = 4.55VYY80 pKa = 10.58DD81 pKa = 3.59GGKK84 pKa = 7.18TLKK87 pKa = 10.04FWEE90 pKa = 4.46EE91 pKa = 3.45NGFPVVSKK99 pKa = 9.12VTVKK103 pKa = 10.77GGPNANIYY111 pKa = 10.26YY112 pKa = 9.79YY113 pKa = 10.94AADD116 pKa = 3.99PPEE119 pKa = 4.36GLGVPVASDD128 pKa = 4.2CGLQSPRR135 pKa = 11.84HH136 pKa = 5.51GKK138 pKa = 9.6NIPGISYY145 pKa = 10.62VEE147 pKa = 4.54FEE149 pKa = 4.99FEE151 pKa = 4.06PPPTGSLKK159 pKa = 10.67VIKK162 pKa = 10.17IWDD165 pKa = 3.7DD166 pKa = 3.05QGTGVVYY173 pKa = 10.79DD174 pKa = 3.37GTIDD178 pKa = 3.53VNIYY182 pKa = 10.47DD183 pKa = 4.09SEE185 pKa = 4.43GTLVDD190 pKa = 3.65TLILSDD196 pKa = 3.71ANNWEE201 pKa = 4.41DD202 pKa = 4.1DD203 pKa = 3.98LGYY206 pKa = 10.65LLPGVYY212 pKa = 9.19TFAEE216 pKa = 4.45VTPDD220 pKa = 2.86GWTPSYY226 pKa = 11.35DD227 pKa = 3.4PANRR231 pKa = 11.84EE232 pKa = 4.14LVVEE236 pKa = 4.76AGDD239 pKa = 4.3DD240 pKa = 3.73PAAGAIGTITNTIDD254 pKa = 3.23LGSLKK259 pKa = 10.45VIKK262 pKa = 10.25IWDD265 pKa = 3.7DD266 pKa = 3.05QGTGVVYY273 pKa = 10.79DD274 pKa = 3.37GTIDD278 pKa = 3.53VNIYY282 pKa = 10.47DD283 pKa = 4.09SEE285 pKa = 4.43GTLVDD290 pKa = 3.76TLILSDD296 pKa = 3.69TNNWEE301 pKa = 4.15DD302 pKa = 3.76DD303 pKa = 3.78LGYY306 pKa = 10.65LLPGVYY312 pKa = 9.19TFAEE316 pKa = 4.45VTPDD320 pKa = 2.47GWTPATTRR328 pKa = 11.84QTVSS332 pKa = 2.66

Molecular weight: 35.9 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C0GHY1|C0GHY1_9FIRM UspA domain protein OS=Dethiobacter alkaliphilus AHT 1 OX=555088 GN=DealDRAFT_2090 PE=3 SV=1
MM1 pKa = 7.59APKK4 pKa = 9.94KK5 pKa = 10.2IKK7 pKa = 10.47GISGSRR13 pKa = 11.84RR14 pKa = 11.84ATYY17 pKa = 10.77APTTDD22 pKa = 4.81IIPSPPQTCDD32 pKa = 2.23GFMYY36 pKa = 9.99VVRR39 pKa = 11.84PRR41 pKa = 11.84DD42 pKa = 3.47TLYY45 pKa = 11.12SIARR49 pKa = 11.84KK50 pKa = 9.23FNCSVMQLLQANPQISSRR68 pKa = 11.84SGTLFIRR75 pKa = 11.84QRR77 pKa = 11.84ICIPDD82 pKa = 3.62VVDD85 pKa = 3.47VLPAHH90 pKa = 6.99KK91 pKa = 10.03LLPAGPKK98 pKa = 9.71VLFVEE103 pKa = 5.33LLDD106 pKa = 4.64SMGNPLRR113 pKa = 11.84VMNGFVSLAPRR124 pKa = 11.84TFIRR128 pKa = 11.84VVFSEE133 pKa = 4.6PVNQVYY139 pKa = 8.7FFFVPSRR146 pKa = 11.84RR147 pKa = 11.84KK148 pKa = 9.05IFRR151 pKa = 11.84PSFLIGEE158 pKa = 4.44RR159 pKa = 11.84TLVPPSRR166 pKa = 11.84SVRR169 pKa = 11.84FVWDD173 pKa = 3.32VPRR176 pKa = 11.84GIRR179 pKa = 11.84GSLFIVGCTEE189 pKa = 4.94RR190 pKa = 11.84ICGPPEE196 pKa = 3.69EE197 pKa = 4.39LLVRR201 pKa = 11.84RR202 pKa = 11.84PP203 pKa = 3.32

Molecular weight: 22.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3162

0

3162

920342

30

1924

291.1

32.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.726 ± 0.054

1.069 ± 0.022

5.198 ± 0.038

7.376 ± 0.059

4.068 ± 0.033

7.597 ± 0.045

1.958 ± 0.018

6.497 ± 0.038

5.258 ± 0.043

10.43 ± 0.058

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.742 ± 0.023

3.69 ± 0.033

4.147 ± 0.029

3.74 ± 0.027

5.241 ± 0.037

5.319 ± 0.029

5.167 ± 0.029

7.741 ± 0.038

0.932 ± 0.017

3.103 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski