Apis mellifera associated microvirus 27

Taxonomy: Viruses; Monodnaviria; Sangervirae; Phixviricota; Malgrandaviricetes; Petitvirales; Microviridae; unclassified Microviridae

Average proteome isoelectric point is 6.9

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3Q8U5B7|A0A3Q8U5B7_9VIRU DNA pilot protein OS=Apis mellifera associated microvirus 27 OX=2494755 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 10.36KK3 pKa = 8.41ITEE6 pKa = 3.91EE7 pKa = 4.62SYY9 pKa = 10.87VEE11 pKa = 4.08TYY13 pKa = 8.01TRR15 pKa = 11.84KK16 pKa = 10.4NGTFGYY22 pKa = 8.65RR23 pKa = 11.84TINNDD28 pKa = 2.34VSMADD33 pKa = 3.29PTGVEE38 pKa = 4.0EE39 pKa = 5.47AEE41 pKa = 4.2ITSIMSNFAKK51 pKa = 10.04TGRR54 pKa = 11.84LPLRR58 pKa = 11.84GGQPGLYY65 pKa = 10.14SPDD68 pKa = 3.07EE69 pKa = 4.09VVPNGKK75 pKa = 9.42DD76 pKa = 3.15LLSAVTLVQEE86 pKa = 3.78ATEE89 pKa = 4.24RR90 pKa = 11.84FEE92 pKa = 4.39QLPSTLRR99 pKa = 11.84EE100 pKa = 3.82RR101 pKa = 11.84FGNDD105 pKa = 3.76PIKK108 pKa = 10.58MYY110 pKa = 11.09DD111 pKa = 3.62FLSDD115 pKa = 3.73PKK117 pKa = 11.33NMPEE121 pKa = 3.74AVKK124 pKa = 10.59LGIIQQPVSKK134 pKa = 10.65NDD136 pKa = 3.45EE137 pKa = 4.31LNDD140 pKa = 5.09DD141 pKa = 4.33IEE143 pKa = 4.71TKK145 pKa = 8.27NTRR148 pKa = 11.84TTKK151 pKa = 7.27TQKK154 pKa = 10.72AKK156 pKa = 10.71EE157 pKa = 4.03KK158 pKa = 9.75TPEE161 pKa = 4.05PEE163 pKa = 3.57

Molecular weight: 18.3 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S8UTQ5|A0A3S8UTQ5_9VIRU Major capsid protein OS=Apis mellifera associated microvirus 27 OX=2494755 PE=3 SV=1
MM1 pKa = 7.25QCINKK6 pKa = 9.4IKK8 pKa = 10.73AGFTRR13 pKa = 11.84TGDD16 pKa = 3.44LTFKK20 pKa = 10.35PRR22 pKa = 11.84QADD25 pKa = 3.36PGIVGFEE32 pKa = 4.11LEE34 pKa = 4.49CRR36 pKa = 11.84KK37 pKa = 10.02CLPCRR42 pKa = 11.84LNIARR47 pKa = 11.84EE48 pKa = 3.7KK49 pKa = 10.58AIRR52 pKa = 11.84AVHH55 pKa = 5.75EE56 pKa = 4.36AEE58 pKa = 4.1CHH60 pKa = 5.48KK61 pKa = 11.16KK62 pKa = 10.67NIFLTLTYY70 pKa = 10.83APGKK74 pKa = 10.05LKK76 pKa = 10.61SPRR79 pKa = 11.84LNYY82 pKa = 10.79GDD84 pKa = 4.06FQLFMKK90 pKa = 10.54RR91 pKa = 11.84LRR93 pKa = 11.84EE94 pKa = 3.81LHH96 pKa = 6.0TRR98 pKa = 11.84DD99 pKa = 4.68VLDD102 pKa = 4.77PDD104 pKa = 4.24LKK106 pKa = 11.09KK107 pKa = 10.95EE108 pKa = 3.87MSISYY113 pKa = 8.46MVTGEE118 pKa = 4.11YY119 pKa = 10.62GDD121 pKa = 3.75KK122 pKa = 10.58NKK124 pKa = 10.21RR125 pKa = 11.84PHH127 pKa = 4.33WHH129 pKa = 7.1AIIFNYY135 pKa = 10.14SPPDD139 pKa = 3.61LMLHH143 pKa = 5.82RR144 pKa = 11.84TDD146 pKa = 3.49QNDD149 pKa = 3.41YY150 pKa = 11.29QIFKK154 pKa = 10.94SKK156 pKa = 10.4IIDD159 pKa = 3.85EE160 pKa = 4.28LWGFNDD166 pKa = 5.12PEE168 pKa = 4.26NCPNEE173 pKa = 4.23IGSVTIEE180 pKa = 3.96SAGYY184 pKa = 7.27VARR187 pKa = 11.84YY188 pKa = 7.94AAKK191 pKa = 10.45KK192 pKa = 8.19LTHH195 pKa = 6.23GQDD198 pKa = 3.33QEE200 pKa = 4.9HH201 pKa = 7.38DD202 pKa = 3.58FHH204 pKa = 6.83PVHH207 pKa = 6.26KK208 pKa = 8.72TSCVNAVGKK217 pKa = 10.3RR218 pKa = 11.84WIEE221 pKa = 3.52KK222 pKa = 9.94HH223 pKa = 4.34YY224 pKa = 10.69QQVFKK229 pKa = 10.87LGYY232 pKa = 8.32VWLPNGGRR240 pKa = 11.84TKK242 pKa = 10.31IPRR245 pKa = 11.84YY246 pKa = 9.27YY247 pKa = 11.03VDD249 pKa = 3.3WLKK252 pKa = 11.18KK253 pKa = 9.72NHH255 pKa = 6.59PEE257 pKa = 3.49EE258 pKa = 4.32HH259 pKa = 6.53KK260 pKa = 11.23NYY262 pKa = 7.56VTKK265 pKa = 10.37IRR267 pKa = 11.84HH268 pKa = 5.71KK269 pKa = 10.19LQLAAAEE276 pKa = 4.16KK277 pKa = 10.54SRR279 pKa = 11.84NEE281 pKa = 3.9EE282 pKa = 3.56IQYY285 pKa = 8.69LTEE288 pKa = 4.98LMNLPHH294 pKa = 7.64GYY296 pKa = 9.8PMPEE300 pKa = 4.26SKK302 pKa = 10.67KK303 pKa = 10.8KK304 pKa = 9.93MSLTILKK311 pKa = 10.16QKK313 pKa = 10.18FKK315 pKa = 11.11KK316 pKa = 10.38LQEE319 pKa = 4.07KK320 pKa = 10.63LKK322 pKa = 10.97LL323 pKa = 3.7

Molecular weight: 37.89 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

1334

84

545

266.8

30.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.946 ± 2.338

0.675 ± 0.487

4.873 ± 0.74

6.072 ± 1.073

5.247 ± 1.437

6.372 ± 0.513

2.324 ± 0.815

4.648 ± 0.467

7.271 ± 2.1

7.946 ± 0.638

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.474 ± 0.544

5.322 ± 0.275

6.297 ± 0.748

5.172 ± 0.618

4.873 ± 0.711

5.922 ± 0.949

6.897 ± 0.894

4.723 ± 0.617

0.975 ± 0.366

3.973 ± 0.321
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski