Human plasma-associated gemycircularvirus

Taxonomy: Viruses; Monodnaviria; Shotokuvirae; Cressdnaviricota; Repensiviricetes; Geplafuvirales; Genomoviridae; Gemyvongvirus; Gemyvongvirus humas1; Human associated gemyvongvirus 1

Average proteome isoelectric point is 7.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0S2C4E4|A0A0S2C4E4_9VIRU Replication-associated protein OS=Human plasma-associated gemycircularvirus OX=1707330 GN=Rep PE=3 SV=1
MM1 pKa = 7.36TNVFPCWSSVTEE13 pKa = 4.19NPSEE17 pKa = 4.63AMTQFQEE24 pKa = 4.43LVSDD28 pKa = 4.25SPRR31 pKa = 11.84GFASSLTVRR40 pKa = 11.84HH41 pKa = 5.9RR42 pKa = 11.84QTLMLTQLCEE52 pKa = 4.37YY53 pKa = 8.53YY54 pKa = 10.41TEE56 pKa = 4.2TAEE59 pKa = 4.15VALSHH64 pKa = 6.87EE65 pKa = 4.39SHH67 pKa = 6.05TWMEE71 pKa = 4.09ALIIMLSWTTEE82 pKa = 3.76HH83 pKa = 7.03LGIGQTLVDD92 pKa = 4.91GMYY95 pKa = 10.39LACTRR100 pKa = 11.84TLSQSRR106 pKa = 11.84EE107 pKa = 3.78LRR109 pKa = 11.84LMHH112 pKa = 6.05MHH114 pKa = 6.05TSEE117 pKa = 3.96RR118 pKa = 11.84TRR120 pKa = 11.84TLFMSVLRR128 pKa = 11.84KK129 pKa = 9.52PLDD132 pKa = 3.15QGLEE136 pKa = 3.99DD137 pKa = 3.76RR138 pKa = 11.84LEE140 pKa = 4.02MLKK143 pKa = 10.6EE144 pKa = 3.52IANAHH149 pKa = 5.63GLRR152 pKa = 11.84SQMHH156 pKa = 3.92QTKK159 pKa = 9.73TSFLRR164 pKa = 11.84HH165 pKa = 4.43ARR167 pKa = 11.84RR168 pKa = 11.84WIRR171 pKa = 11.84EE172 pKa = 4.2VLSHH176 pKa = 7.31ASGTFPDD183 pKa = 3.94TQITPFQVYY192 pKa = 9.86EE193 pKa = 4.79DD194 pKa = 3.87NTSPQPGSPLDD205 pKa = 3.49SLSIHH210 pKa = 6.47DD211 pKa = 5.56LEE213 pKa = 5.49NISPIGSDD221 pKa = 3.25NEE223 pKa = 4.4GGKK226 pKa = 7.24TTLTLTLTRR235 pKa = 11.84KK236 pKa = 10.27GNEE239 pKa = 4.19LGPNGPGLSPNPLTLALTLTLTLANQLGQTYY270 pKa = 7.94LTHH273 pKa = 7.87AMGSFQNRR281 pKa = 11.84EE282 pKa = 4.0DD283 pKa = 3.37PTRR286 pKa = 11.84PLVRR290 pKa = 11.84TAA292 pKa = 4.1

Molecular weight: 32.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0S2C3Q0|A0A0S2C3Q0_9VIRU Putative ORF3 protein OS=Human plasma-associated gemycircularvirus OX=1707330 GN=ORF3 PE=4 SV=1
MM1 pKa = 7.8AYY3 pKa = 10.21ARR5 pKa = 11.84YY6 pKa = 9.31RR7 pKa = 11.84RR8 pKa = 11.84RR9 pKa = 11.84RR10 pKa = 11.84PYY12 pKa = 8.53RR13 pKa = 11.84TNRR16 pKa = 11.84KK17 pKa = 6.35RR18 pKa = 11.84TSYY21 pKa = 10.41RR22 pKa = 11.84RR23 pKa = 11.84NTRR26 pKa = 11.84RR27 pKa = 11.84TRR29 pKa = 11.84NAPFRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84SYY38 pKa = 10.43RR39 pKa = 11.84RR40 pKa = 11.84PANRR44 pKa = 11.84FKK46 pKa = 11.08KK47 pKa = 9.92RR48 pKa = 11.84VLAISSKK55 pKa = 10.93KK56 pKa = 10.59KK57 pKa = 9.76FDD59 pKa = 3.42TMRR62 pKa = 11.84SGSANGVIDD71 pKa = 3.45VTFNDD76 pKa = 3.41VQQAIMAWSPTTIPYY91 pKa = 10.53NEE93 pKa = 4.57VEE95 pKa = 4.44HH96 pKa = 7.04SPAHH100 pKa = 6.59RR101 pKa = 11.84NSQQIFFTGVKK112 pKa = 8.3EE113 pKa = 4.22TLDD116 pKa = 3.78PYY118 pKa = 11.21AIFPFHH124 pKa = 6.4WRR126 pKa = 11.84RR127 pKa = 11.84VVFWTHH133 pKa = 4.45EE134 pKa = 3.97RR135 pKa = 11.84FPQFEE140 pKa = 4.61TIVAAGGTRR149 pKa = 11.84FRR151 pKa = 11.84YY152 pKa = 9.2GVTYY156 pKa = 10.42GVTDD160 pKa = 3.69TPVQAIQEE168 pKa = 4.21LMFQGTIGIDD178 pKa = 3.37WDD180 pKa = 3.55PTRR183 pKa = 11.84PMDD186 pKa = 4.79ARR188 pKa = 11.84LDD190 pKa = 3.42RR191 pKa = 11.84HH192 pKa = 6.38RR193 pKa = 11.84IKK195 pKa = 10.44IVSDD199 pKa = 2.89KK200 pKa = 10.71RR201 pKa = 11.84VNFNPQSDD209 pKa = 3.45NGRR212 pKa = 11.84YY213 pKa = 9.15SVFKK217 pKa = 8.88RR218 pKa = 11.84WHH220 pKa = 5.55SIRR223 pKa = 11.84RR224 pKa = 11.84RR225 pKa = 11.84IMYY228 pKa = 9.44DD229 pKa = 3.05QEE231 pKa = 4.12EE232 pKa = 4.47AGDD235 pKa = 4.04DD236 pKa = 3.79QISDD240 pKa = 3.33VWSTVAPTSSGNLFIVDD257 pKa = 4.0FFGFPPGLAQGAAARR272 pKa = 11.84LGLQSTVYY280 pKa = 9.57WRR282 pKa = 11.84EE283 pKa = 3.74SS284 pKa = 3.1

Molecular weight: 33.36 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

917

284

341

305.7

35.02

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.78 ± 0.471

1.2 ± 0.617

5.78 ± 1.233

4.689 ± 0.842

5.016 ± 0.889

6.979 ± 1.094

2.944 ± 0.455

4.253 ± 0.529

2.835 ± 0.44

7.197 ± 2.535

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.29 ± 0.868

3.926 ± 0.108

5.562 ± 0.033

4.253 ± 0.554

9.815 ± 1.498

7.415 ± 0.597

8.724 ± 1.504

5.453 ± 0.794

2.181 ± 0.341

3.708 ± 0.874
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski