Human respirovirus 3

Taxonomy: Viruses; Riboviria; Orthornavirae; Negarnaviricota; Haploviricotina; Monjiviricetes; Mononegavirales; Paramyxoviridae; Orthoparamyxovirinae; Respirovirus

Average proteome isoelectric point is 6.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 8 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|T1UFE7|T1UFE7_9MONO Nucleocapsid OS=Human respirovirus 3 OX=11216 GN=N PE=3 SV=1
MM1 pKa = 7.66LSLFDD6 pKa = 3.51TFNARR11 pKa = 11.84RR12 pKa = 11.84QEE14 pKa = 4.42NITKK18 pKa = 10.13SAGGAIIPGQKK29 pKa = 8.49NTVSIFALGPTITDD43 pKa = 3.78DD44 pKa = 3.84NEE46 pKa = 4.12KK47 pKa = 8.4MTLALLFLSHH57 pKa = 6.91SLDD60 pKa = 3.36NEE62 pKa = 4.0KK63 pKa = 10.13QHH65 pKa = 5.9AQRR68 pKa = 11.84AGFLVSLLSMAYY80 pKa = 10.11ANPEE84 pKa = 4.11LYY86 pKa = 9.54LTTNGSNADD95 pKa = 3.65VKK97 pKa = 10.62YY98 pKa = 10.03VIYY101 pKa = 9.75MIEE104 pKa = 4.4KK105 pKa = 9.19DD106 pKa = 4.42LKK108 pKa = 7.46RR109 pKa = 11.84QKK111 pKa = 10.92YY112 pKa = 9.39GGFVVKK118 pKa = 9.19TRR120 pKa = 11.84EE121 pKa = 3.85MIYY124 pKa = 10.74EE125 pKa = 4.24KK126 pKa = 8.71TTDD129 pKa = 3.53WIFGSDD135 pKa = 3.59LDD137 pKa = 4.24YY138 pKa = 11.4DD139 pKa = 4.09QEE141 pKa = 4.43TMLQNGRR148 pKa = 11.84NNSTIEE154 pKa = 4.1DD155 pKa = 3.8LVHH158 pKa = 5.73TFGYY162 pKa = 9.16PSCLGAIIIQIWIVLVKK179 pKa = 10.55AITSISGLRR188 pKa = 11.84KK189 pKa = 9.95GFFTRR194 pKa = 11.84LEE196 pKa = 4.02AFRR199 pKa = 11.84QDD201 pKa = 3.09GTVQAGLVLSGDD213 pKa = 3.79TVDD216 pKa = 4.06QIGSIMRR223 pKa = 11.84SQQSLVTLMVEE234 pKa = 4.35TLITMNTSRR243 pKa = 11.84NDD245 pKa = 2.98LTTIEE250 pKa = 4.61KK251 pKa = 10.41NIQIVGNYY259 pKa = 8.94IRR261 pKa = 11.84DD262 pKa = 3.47AGLASFFNTIRR273 pKa = 11.84YY274 pKa = 9.22GIEE277 pKa = 3.27TRR279 pKa = 11.84MAALTLSTLRR289 pKa = 11.84PDD291 pKa = 3.44INRR294 pKa = 11.84LKK296 pKa = 11.05ALMEE300 pKa = 4.81LYY302 pKa = 10.37LSKK305 pKa = 10.93GPRR308 pKa = 11.84APFICILRR316 pKa = 11.84DD317 pKa = 4.01PIHH320 pKa = 7.17GEE322 pKa = 4.01FAPGNYY328 pKa = 8.42PAIWSYY334 pKa = 11.86AMGVAVVQNRR344 pKa = 11.84AMQQYY349 pKa = 7.78VTGRR353 pKa = 11.84SYY355 pKa = 11.82LDD357 pKa = 2.77IDD359 pKa = 3.9MFQLGQAVARR369 pKa = 11.84DD370 pKa = 4.05AEE372 pKa = 4.34AQMSSTLEE380 pKa = 4.14DD381 pKa = 3.65EE382 pKa = 5.37LGVTHH387 pKa = 6.45EE388 pKa = 4.81AKK390 pKa = 10.53EE391 pKa = 4.05SLKK394 pKa = 10.74RR395 pKa = 11.84HH396 pKa = 4.97IRR398 pKa = 11.84NINSSDD404 pKa = 3.39TSFHH408 pKa = 6.63KK409 pKa = 9.67PTGGSAIEE417 pKa = 3.91MAIDD421 pKa = 3.52EE422 pKa = 4.56EE423 pKa = 4.66PEE425 pKa = 4.04QFEE428 pKa = 4.06NRR430 pKa = 11.84ADD432 pKa = 3.69QEE434 pKa = 4.25QDD436 pKa = 2.96GGPQSSIIQYY446 pKa = 10.37AWAEE450 pKa = 4.29GNRR453 pKa = 11.84SDD455 pKa = 5.87DD456 pKa = 3.59RR457 pKa = 11.84TEE459 pKa = 3.74QATEE463 pKa = 3.58SDD465 pKa = 4.0NIKK468 pKa = 9.5TEE470 pKa = 3.81QQNIRR475 pKa = 11.84DD476 pKa = 4.04RR477 pKa = 11.84LNKK480 pKa = 9.99RR481 pKa = 11.84LNDD484 pKa = 3.55KK485 pKa = 10.24KK486 pKa = 10.89KK487 pKa = 10.46QGSQPPTNPTNRR499 pKa = 11.84TNQDD503 pKa = 3.38EE504 pKa = 4.24IDD506 pKa = 3.84DD507 pKa = 4.33LFNAFGSNN515 pKa = 3.14

Molecular weight:
57.71 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|T1UFE7|T1UFE7_9MONO Nucleocapsid OS=Human respirovirus 3 OX=11216 GN=N PE=3 SV=1
MM1 pKa = 7.77SITNSAIYY9 pKa = 8.25TFPEE13 pKa = 4.1SSFSEE18 pKa = 4.16NGHH21 pKa = 6.56IEE23 pKa = 4.0PLPLKK28 pKa = 10.87VNEE31 pKa = 3.85QRR33 pKa = 11.84KK34 pKa = 8.51AVPHH38 pKa = 5.81IRR40 pKa = 11.84VAKK43 pKa = 9.94IGNPPKK49 pKa = 10.43HH50 pKa = 6.24GSRR53 pKa = 11.84YY54 pKa = 10.04LDD56 pKa = 3.28VFLLGFFEE64 pKa = 4.38MEE66 pKa = 4.46RR67 pKa = 11.84IKK69 pKa = 11.03DD70 pKa = 3.49KK71 pKa = 11.28YY72 pKa = 11.22GSVNDD77 pKa = 4.42LDD79 pKa = 4.65SDD81 pKa = 4.03PSYY84 pKa = 10.74KK85 pKa = 10.39VCGSGSLPIGLAKK98 pKa = 9.84YY99 pKa = 8.27TGNDD103 pKa = 3.24QEE105 pKa = 4.84LLQAATKK112 pKa = 10.69LDD114 pKa = 3.32IEE116 pKa = 4.43VRR118 pKa = 11.84RR119 pKa = 11.84TVKK122 pKa = 10.28AKK124 pKa = 10.32EE125 pKa = 3.87MVVYY129 pKa = 8.58TVQNIKK135 pKa = 10.55PEE137 pKa = 4.47LYY139 pKa = 8.73PWSSRR144 pKa = 11.84LRR146 pKa = 11.84KK147 pKa = 10.12GMLFDD152 pKa = 4.35ANKK155 pKa = 10.02VALAPQCLPLDD166 pKa = 3.63RR167 pKa = 11.84SIKK170 pKa = 9.94FRR172 pKa = 11.84VIFVNCTAIGSITLFKK188 pKa = 10.26IPKK191 pKa = 9.75SMASLSLPNTISINLQVHH209 pKa = 6.54IKK211 pKa = 9.74TGVQTDD217 pKa = 3.52SKK219 pKa = 11.19GVVQILDD226 pKa = 3.61EE227 pKa = 4.79KK228 pKa = 11.28GEE230 pKa = 3.98KK231 pKa = 9.96SLNFMVHH238 pKa = 6.48LGLIKK243 pKa = 10.42RR244 pKa = 11.84KK245 pKa = 8.58VGRR248 pKa = 11.84MYY250 pKa = 10.77SVEE253 pKa = 3.94YY254 pKa = 10.17CKK256 pKa = 10.57QKK258 pKa = 9.94IEE260 pKa = 3.94KK261 pKa = 8.72MRR263 pKa = 11.84LIFSLGLVGGISLHH277 pKa = 5.86VNATGSISKK286 pKa = 8.54TLASQLVFKK295 pKa = 11.15RR296 pKa = 11.84EE297 pKa = 3.37ICYY300 pKa = 10.21PLMDD304 pKa = 5.92LNPHH308 pKa = 6.45LNLVIWASSVEE319 pKa = 4.04ITRR322 pKa = 11.84VDD324 pKa = 4.73AIFQPSLPGEE334 pKa = 3.75FRR336 pKa = 11.84YY337 pKa = 10.34YY338 pKa = 10.39PNIIAKK344 pKa = 9.88GVGKK348 pKa = 10.33IKK350 pKa = 10.11QWNN353 pKa = 3.35

Molecular weight:
39.53 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

8

0

8

5386

199

2233

673.3

76.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.233 ± 0.466

1.355 ± 0.215

6.127 ± 0.398

5.997 ± 0.462

2.822 ± 0.52

5.217 ± 0.354

1.875 ± 0.159

9.005 ± 0.573

6.907 ± 0.465

9.543 ± 1.011

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.321 ± 0.184

6.424 ± 0.306

3.695 ± 0.254

4.475 ± 0.971

4.976 ± 0.443

8.708 ± 0.622

6.944 ± 0.854

4.846 ± 0.406

1.133 ± 0.223

3.398 ± 0.567

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski