Virgibacillus salinus

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Virgibacillus

Average proteome isoelectric point is 6.06

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3649 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H1CLZ6|A0A1H1CLZ6_9BACI NAD(P)-dependent dehydrogenase short-chain alcohol dehydrogenase family OS=Virgibacillus salinus OX=553311 GN=SAMN05216231_2308 PE=4 SV=1
MM1 pKa = 7.3ARR3 pKa = 11.84IGVEE7 pKa = 3.96EE8 pKa = 4.24SLSDD12 pKa = 3.71VKK14 pKa = 11.09DD15 pKa = 3.69GLMEE19 pKa = 4.34MGHH22 pKa = 6.57DD23 pKa = 3.76VVDD26 pKa = 3.99LHH28 pKa = 9.22SEE30 pKa = 4.17DD31 pKa = 3.39DD32 pKa = 4.04TEE34 pKa = 4.92YY35 pKa = 11.17CDD37 pKa = 3.63CCVITGQDD45 pKa = 3.55KK46 pKa = 11.15NVMGMSTTSIAGPVINAEE64 pKa = 4.36GYY66 pKa = 9.44SAEE69 pKa = 4.6EE70 pKa = 4.08VCQMVNQKK78 pKa = 10.99LNGNEE83 pKa = 3.72

Molecular weight: 8.96 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H0XRZ8|A0A1H0XRZ8_9BACI Sugar phosphate isomerase/epimerase OS=Virgibacillus salinus OX=553311 GN=SAMN05216231_0151 PE=4 SV=1
MM1 pKa = 7.44KK2 pKa = 9.6RR3 pKa = 11.84TFQPNNRR10 pKa = 11.84KK11 pKa = 9.23RR12 pKa = 11.84KK13 pKa = 8.12KK14 pKa = 8.46VHH16 pKa = 5.5GFRR19 pKa = 11.84TRR21 pKa = 11.84MSSKK25 pKa = 10.41NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84KK37 pKa = 9.05GRR39 pKa = 11.84KK40 pKa = 8.7VLSAA44 pKa = 4.05

Molecular weight: 5.32 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3649

0

3649

1048091

31

1939

287.2

32.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.658 ± 0.045

0.618 ± 0.012

5.558 ± 0.034

7.492 ± 0.049

4.527 ± 0.035

6.821 ± 0.044

2.001 ± 0.022

8.15 ± 0.047

6.841 ± 0.04

9.375 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.822 ± 0.022

4.96 ± 0.032

3.5 ± 0.026

3.724 ± 0.026

3.757 ± 0.03

6.163 ± 0.03

5.499 ± 0.029

6.933 ± 0.032

1.022 ± 0.015

3.578 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski