Vibrio phage helene 12B3

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; unclassified Myoviridae

Average proteome isoelectric point is 5.93

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 263 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|M4M930|M4M930_9CAUD Uncharacterized protein OS=Vibrio phage helene 12B3 OX=573173 GN=VPBG_00080 PE=4 SV=1
MM1 pKa = 7.6TSTEE5 pKa = 3.98PLIGKK10 pKa = 6.93TFVCTDD16 pKa = 3.98PDD18 pKa = 3.83FKK20 pKa = 11.5GCVGTVEE27 pKa = 5.67DD28 pKa = 4.14IWFEE32 pKa = 4.14DD33 pKa = 4.45GIPYY37 pKa = 10.27EE38 pKa = 4.5FIRR41 pKa = 11.84VVGEE45 pKa = 4.13DD46 pKa = 3.52FSGTFLFNEE55 pKa = 5.65GYY57 pKa = 9.65IQNDD61 pKa = 3.67YY62 pKa = 11.42

Molecular weight: 7.05 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|M4MC93|M4MC93_9CAUD Uncharacterized protein OS=Vibrio phage helene 12B3 OX=573173 GN=VPBG_00149 PE=4 SV=1
MM1 pKa = 6.89SQRR4 pKa = 11.84TIKK7 pKa = 10.59ASQKK11 pKa = 9.64RR12 pKa = 11.84AQQRR16 pKa = 11.84IIRR19 pKa = 11.84NKK21 pKa = 9.53SMAAMRR27 pKa = 11.84VKK29 pKa = 10.45SQPQDD34 pKa = 3.15RR35 pKa = 11.84AEE37 pKa = 3.96VLSTIKK43 pKa = 10.37RR44 pKa = 11.84INVVRR49 pKa = 11.84GG50 pKa = 3.23

Molecular weight: 5.77 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

263

0

263

39730

42

1079

151.1

17.28

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

4.923 ± 0.212

1.84 ± 0.1

6.597 ± 0.139

8.137 ± 0.191

4.249 ± 0.108

6.32 ± 0.149

2.006 ± 0.093

5.719 ± 0.126

8.024 ± 0.212

8.58 ± 0.185

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.686 ± 0.108

4.928 ± 0.138

2.927 ± 0.101

3.549 ± 0.1

4.196 ± 0.121

6.587 ± 0.168

6.139 ± 0.158

6.662 ± 0.15

1.611 ± 0.071

4.319 ± 0.124
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski