Labedaea rhizosphaerae

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Actinobacteria; Actinomycetia; Pseudonocardiales; Pseudonocardiaceae; Labedaea

Average proteome isoelectric point is 6.23

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7717 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4R6SGB8|A0A4R6SGB8_9PSEU Pimeloyl-ACP methyl ester carboxylesterase OS=Labedaea rhizosphaerae OX=598644 GN=EV186_102448 PE=4 SV=1
MM1 pKa = 7.34SLDD4 pKa = 3.61PTTLQSFVTNLLTDD18 pKa = 4.03DD19 pKa = 4.03AARR22 pKa = 11.84SAFAADD28 pKa = 3.6PTAVLQGAGLGDD40 pKa = 3.58ITPQDD45 pKa = 3.87VQQVIPLVTDD55 pKa = 3.12QLPGLDD61 pKa = 4.17GGLPTLPALPTEE73 pKa = 4.33QVNDD77 pKa = 3.82AVAQLQAIADD87 pKa = 3.86AAQANSGLPLPHH99 pKa = 7.35VGFASTSDD107 pKa = 3.28QGNLAGAVTVTGDD120 pKa = 3.14GVTGGGAASAGVDD133 pKa = 3.55GVGVTGGVDD142 pKa = 3.21SAYY145 pKa = 9.41GTAHH149 pKa = 6.54GSGVVGLDD157 pKa = 3.06GGEE160 pKa = 4.42GNLTAASHH168 pKa = 6.06QLHH171 pKa = 5.94TWSASDD177 pKa = 3.96ASVGLDD183 pKa = 3.14GAAAATSTHH192 pKa = 6.1VYY194 pKa = 10.86DD195 pKa = 4.05NAVSGAATVTPDD207 pKa = 3.33GNFTAVGGAHH217 pKa = 6.84SDD219 pKa = 3.27LGDD222 pKa = 3.32VHH224 pKa = 6.6GAVAGGLDD232 pKa = 3.48GVYY235 pKa = 10.92GNGGVHH241 pKa = 6.62TDD243 pKa = 4.09DD244 pKa = 5.27LATNTDD250 pKa = 3.93FHH252 pKa = 6.34ATQDD256 pKa = 3.43GFGVVTNGYY265 pKa = 9.73GSQLGSFQSAAAGSTDD281 pKa = 3.47RR282 pKa = 11.84VATHH286 pKa = 7.0NEE288 pKa = 3.77FQTANAQGSNDD299 pKa = 3.3FSVGKK304 pKa = 10.3DD305 pKa = 3.37GLATSSTLQTDD316 pKa = 3.9QVSAASDD323 pKa = 3.46ATVGTDD329 pKa = 3.34GTFTSTTAADD339 pKa = 3.65SQFGGFSATASGSTQGFAGATEE361 pKa = 3.83FHH363 pKa = 6.54GAGVNGGGGVAAGLDD378 pKa = 3.91GVTGKK383 pKa = 10.61AGLDD387 pKa = 3.47SQFGHH392 pKa = 6.48VDD394 pKa = 3.04VAGAVSTSGGAGALAVGNDD413 pKa = 3.5MLGVEE418 pKa = 4.68GDD420 pKa = 3.8VAATTDD426 pKa = 3.84SVSLGGDD433 pKa = 3.54LHH435 pKa = 6.49TPAGDD440 pKa = 4.04LPLTPVNIPVGLPGVDD456 pKa = 3.45GLPGVGGLTGALPGVGALPGVDD478 pKa = 3.89GLTGALPGVDD488 pKa = 4.08GLTGALPVDD497 pKa = 4.26GLEE500 pKa = 4.54HH501 pKa = 6.95GLPDD505 pKa = 3.85VDD507 pKa = 4.18GLTGAVPGVDD517 pKa = 3.77GLTGALPLDD526 pKa = 4.04GLTNGGLPGVDD537 pKa = 3.71GLTGALPVDD546 pKa = 4.26ALSGALPLADD556 pKa = 3.36QFGGLGDD563 pKa = 3.9PAHH566 pKa = 6.86ILDD569 pKa = 4.67SIEE572 pKa = 3.81PARR575 pKa = 11.84GGEE578 pKa = 4.02AAVADD583 pKa = 4.15YY584 pKa = 10.94VSNGGQVLTDD594 pKa = 3.7GLATGTNTLGQYY606 pKa = 8.44LTGTPAAPVADD617 pKa = 3.84VVEE620 pKa = 4.66TGSNTVSGIVSQGADD635 pKa = 3.29VASHH639 pKa = 6.13VPALPAVSDD648 pKa = 4.33LPSHH652 pKa = 6.65LPVGDD657 pKa = 4.62LPAASDD663 pKa = 4.22LPNLSDD669 pKa = 4.71LPSHH673 pKa = 6.89LPVDD677 pKa = 4.57LPNLPVANPLPEE689 pKa = 4.3VTQHH693 pKa = 4.93VTDD696 pKa = 4.27LTSHH700 pKa = 5.26VTDD703 pKa = 3.39VTSTVGNVTDD713 pKa = 3.96VVTHH717 pKa = 6.1NPVTDD722 pKa = 4.01VVSHH726 pKa = 6.35SPLGNVTGGDD736 pKa = 3.4HH737 pKa = 6.86GLLGGDD743 pKa = 3.62GLLPDD748 pKa = 4.54LHH750 pKa = 7.48LGHH753 pKa = 7.41

Molecular weight: 72.25 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4R6SRF3|A0A4R6SRF3_9PSEU Bifunctional purine biosynthesis protein PurH OS=Labedaea rhizosphaerae OX=598644 GN=purH PE=3 SV=1
MM1 pKa = 7.4GSVIKK6 pKa = 10.38KK7 pKa = 8.42RR8 pKa = 11.84RR9 pKa = 11.84KK10 pKa = 9.06RR11 pKa = 11.84MSKK14 pKa = 9.76KK15 pKa = 9.54KK16 pKa = 9.79HH17 pKa = 5.67RR18 pKa = 11.84KK19 pKa = 8.46LLRR22 pKa = 11.84KK23 pKa = 7.95TRR25 pKa = 11.84VQRR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 9.97LKK32 pKa = 10.51KK33 pKa = 9.87

Molecular weight: 4.19 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7717

0

7717

2529000

29

8347

327.7

35.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.492 ± 0.049

0.791 ± 0.008

6.333 ± 0.023

5.268 ± 0.027

2.795 ± 0.015

9.104 ± 0.032

2.3 ± 0.013

3.397 ± 0.02

2.176 ± 0.023

10.34 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.798 ± 0.012

1.926 ± 0.016

5.874 ± 0.025

2.977 ± 0.018

7.516 ± 0.036

5.016 ± 0.021

6.262 ± 0.028

9.116 ± 0.029

1.545 ± 0.011

1.976 ± 0.016
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski