Bacillus phage W.Ph.

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Herelleviridae; Bastillevirinae; Wphvirus; Bacillus virus WPh

Average proteome isoelectric point is 6.12

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 274 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G9B1S8|G9B1S8_9CAUD Gp177 OS=Bacillus phage W.Ph. OX=764595 PE=4 SV=1
MM1 pKa = 7.85RR2 pKa = 11.84MKK4 pKa = 10.57KK5 pKa = 9.86VDD7 pKa = 3.68KK8 pKa = 9.53LTAAIEE14 pKa = 4.2MVKK17 pKa = 10.5GLEE20 pKa = 4.32VKK22 pKa = 10.78CGGLEE27 pKa = 4.22VNWLEE32 pKa = 4.78STDD35 pKa = 3.36EE36 pKa = 3.92WVINYY41 pKa = 9.97GDD43 pKa = 4.32VIIMDD48 pKa = 5.09DD49 pKa = 3.61LTEE52 pKa = 4.07EE53 pKa = 3.95QAYY56 pKa = 8.99EE57 pKa = 4.01CHH59 pKa = 6.1GTLTQYY65 pKa = 9.98EE66 pKa = 4.47VQGVQVEE73 pKa = 4.38LKK75 pKa = 10.04EE76 pKa = 4.09YY77 pKa = 9.91NYY79 pKa = 10.9DD80 pKa = 3.4YY81 pKa = 11.89AMEE84 pKa = 4.41NNKK87 pKa = 10.11GEE89 pKa = 4.39SFTEE93 pKa = 3.7IALEE97 pKa = 4.5RR98 pKa = 11.84MVIDD102 pKa = 3.64DD103 pKa = 3.99WGSDD107 pKa = 3.46FEE109 pKa = 4.62TVAFEE114 pKa = 4.29VADD117 pKa = 4.0GVTPFANYY125 pKa = 9.15EE126 pKa = 3.7IWEE129 pKa = 4.4VASNNYY135 pKa = 9.51EE136 pKa = 4.22SIEE139 pKa = 4.0EE140 pKa = 4.21NIEE143 pKa = 3.63EE144 pKa = 4.18GLIVIDD150 pKa = 5.19GEE152 pKa = 4.39LNKK155 pKa = 10.7NFMASIAYY163 pKa = 7.44TLTRR167 pKa = 11.84SIEE170 pKa = 4.04QNKK173 pKa = 10.1EE174 pKa = 3.79EE175 pKa = 4.32ILYY178 pKa = 10.6NYY180 pKa = 8.98IAYY183 pKa = 9.59RR184 pKa = 11.84VNEE187 pKa = 4.07MLVKK191 pKa = 10.53KK192 pKa = 10.37RR193 pKa = 11.84VTDD196 pKa = 3.71EE197 pKa = 4.46NIIEE201 pKa = 4.11YY202 pKa = 10.63LEE204 pKa = 4.12EE205 pKa = 4.52EE206 pKa = 4.22IEE208 pKa = 4.27NYY210 pKa = 10.51ADD212 pKa = 3.53EE213 pKa = 5.45AEE215 pKa = 4.08NSYY218 pKa = 11.68NEE220 pKa = 4.11FNYY223 pKa = 10.68LDD225 pKa = 4.85DD226 pKa = 4.61EE227 pKa = 4.49LFEE230 pKa = 4.03QLEE233 pKa = 4.34EE234 pKa = 5.06FIDD237 pKa = 4.05SYY239 pKa = 11.41IAGLSMGEE247 pKa = 4.02SLGNQDD253 pKa = 3.85YY254 pKa = 11.45EE255 pKa = 4.58NIGDD259 pKa = 3.78VTVTEE264 pKa = 4.23GAMFIKK270 pKa = 10.26KK271 pKa = 10.43DD272 pKa = 3.3EE273 pKa = 4.62DD274 pKa = 3.78TDD276 pKa = 3.24KK277 pKa = 10.92SYY279 pKa = 11.66YY280 pKa = 8.52VVRR283 pKa = 11.84VCGNEE288 pKa = 3.35FDD290 pKa = 5.12GFYY293 pKa = 10.27LDD295 pKa = 5.47SMYY298 pKa = 10.73IDD300 pKa = 5.85LNDD303 pKa = 3.99GWIDD307 pKa = 3.25WYY309 pKa = 10.94AINDD313 pKa = 3.63YY314 pKa = 11.38AGIDD318 pKa = 3.78MDD320 pKa = 4.89ADD322 pKa = 3.67DD323 pKa = 4.56YY324 pKa = 11.87EE325 pKa = 4.49RR326 pKa = 11.84VNALISYY333 pKa = 10.25DD334 pKa = 4.03GIDD337 pKa = 3.78NFNPSTTEE345 pKa = 3.67CKK347 pKa = 9.39TAIDD351 pKa = 3.67LCRR354 pKa = 11.84ALRR357 pKa = 11.84EE358 pKa = 4.1AGVNVVWSAEE368 pKa = 3.69HH369 pKa = 7.51SEE371 pKa = 4.07IQFF374 pKa = 3.45

Molecular weight: 43.16 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G9B1C1|G9B1C1_9CAUD Gp20 OS=Bacillus phage W.Ph. OX=764595 PE=4 SV=1
MM1 pKa = 7.27ARR3 pKa = 11.84KK4 pKa = 8.54PRR6 pKa = 11.84KK7 pKa = 8.19PRR9 pKa = 11.84KK10 pKa = 9.35VPKK13 pKa = 9.95LLQGGQEE20 pKa = 3.93TSRR23 pKa = 11.84AINNIGKK30 pKa = 9.61ALQQQALQSTANQLSKK46 pKa = 10.87SLPEE50 pKa = 4.41GYY52 pKa = 9.51EE53 pKa = 3.66LKK55 pKa = 10.39QRR57 pKa = 11.84PKK59 pKa = 10.12YY60 pKa = 10.98LEE62 pKa = 3.91VTEE65 pKa = 4.42KK66 pKa = 10.77RR67 pKa = 11.84LIGMGVIDD75 pKa = 6.02LKK77 pKa = 11.22PLFAKK82 pKa = 10.36SSKK85 pKa = 10.61KK86 pKa = 10.34KK87 pKa = 10.09FSKK90 pKa = 10.45KK91 pKa = 10.26GGWYY95 pKa = 9.93LDD97 pKa = 3.03IPIRR101 pKa = 11.84RR102 pKa = 11.84KK103 pKa = 9.96ARR105 pKa = 11.84GMTRR109 pKa = 11.84RR110 pKa = 11.84MYY112 pKa = 10.55DD113 pKa = 2.9QLRR116 pKa = 11.84AVDD119 pKa = 3.91MGGQQKK125 pKa = 10.4INIISDD131 pKa = 3.46YY132 pKa = 10.78LYY134 pKa = 10.86DD135 pKa = 3.68NRR137 pKa = 11.84GVSDD141 pKa = 4.72APLLNYY147 pKa = 9.51KK148 pKa = 9.89PKK150 pKa = 10.98SNVITKK156 pKa = 9.75IASGKK161 pKa = 9.42GRR163 pKa = 11.84HH164 pKa = 6.64DD165 pKa = 3.29YY166 pKa = 10.67VAFRR170 pKa = 11.84RR171 pKa = 11.84VSNNSSPSSWIINRR185 pKa = 11.84DD186 pKa = 3.12RR187 pKa = 11.84VTVANTSKK195 pKa = 9.8TFVANVNRR203 pKa = 11.84LMKK206 pKa = 10.94YY207 pKa = 9.52NMKK210 pKa = 10.75NMM212 pKa = 3.89

Molecular weight: 24.18 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

274

0

274

48101

32

1978

175.6

20.07

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.491 ± 0.241

0.902 ± 0.078

6.559 ± 0.149

8.27 ± 0.29

3.929 ± 0.087

5.969 ± 0.255

1.867 ± 0.09

6.767 ± 0.134

8.224 ± 0.172

7.79 ± 0.159

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.114 ± 0.093

5.736 ± 0.161

2.761 ± 0.129

3.056 ± 0.124

4.447 ± 0.106

5.969 ± 0.129

6.33 ± 0.206

7.131 ± 0.157

1.156 ± 0.066

4.532 ± 0.12
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski