Erysiphe necator mitovirus 3

Taxonomy: Viruses; Riboviria; Orthornavirae; Lenarviricota; Howeltoviricetes; Cryppavirales; Mitoviridae; Mitovirus; unclassified Mitovirus

Average proteome isoelectric point is 8.52

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2I5ARF2|A0A2I5ARF2_9VIRU RNA-dependent RNA polymerase OS=Erysiphe necator mitovirus 3 OX=2052563 PE=4 SV=1
MM1 pKa = 7.71RR2 pKa = 11.84GVDD5 pKa = 3.37NTIQRR10 pKa = 11.84IKK12 pKa = 10.65DD13 pKa = 3.57SKK15 pKa = 10.72LHH17 pKa = 5.05ITRR20 pKa = 11.84YY21 pKa = 8.23MCGQALYY28 pKa = 10.94SSINPSVGINKK39 pKa = 9.7KK40 pKa = 10.17GLPIWIGTEE49 pKa = 3.92MNKK52 pKa = 10.28LADD55 pKa = 3.66GDD57 pKa = 3.64KK58 pKa = 9.98WEE60 pKa = 4.37KK61 pKa = 10.64RR62 pKa = 11.84LLLTLLSVSRR72 pKa = 11.84ALPGSQYY79 pKa = 10.5IPPLSSLSDD88 pKa = 3.09KK89 pKa = 9.73PTYY92 pKa = 10.47KK93 pKa = 9.98EE94 pKa = 4.32DD95 pKa = 4.17KK96 pKa = 8.04ISEE99 pKa = 4.43YY100 pKa = 10.2ISYY103 pKa = 10.44IPMVMQMMNIPSKK116 pKa = 11.02INVKK120 pKa = 8.59WRR122 pKa = 11.84QFHH125 pKa = 6.12LTTKK129 pKa = 10.33KK130 pKa = 10.77GPNGQAMVSSIRR142 pKa = 11.84DD143 pKa = 3.33AHH145 pKa = 6.97LLDD148 pKa = 5.66DD149 pKa = 5.65GMLKK153 pKa = 10.47DD154 pKa = 3.51IRR156 pKa = 11.84TVAGDD161 pKa = 3.7EE162 pKa = 4.03IIEE165 pKa = 4.49VINHH169 pKa = 5.45CRR171 pKa = 11.84EE172 pKa = 3.92LDD174 pKa = 3.69PEE176 pKa = 4.34SVEE179 pKa = 4.48VVAKK183 pKa = 10.96SMVKK187 pKa = 10.41PSTATLNEE195 pKa = 4.37DD196 pKa = 3.33KK197 pKa = 11.36GMDD200 pKa = 3.59KK201 pKa = 10.82PSTLLRR207 pKa = 11.84KK208 pKa = 10.06LSIVHH213 pKa = 5.77APEE216 pKa = 4.1RR217 pKa = 11.84KK218 pKa = 9.33SRR220 pKa = 11.84IIAILDD226 pKa = 3.25YY227 pKa = 8.25WTQSSLKK234 pKa = 8.84PLHH237 pKa = 6.83DD238 pKa = 5.31RR239 pKa = 11.84IFSILKK245 pKa = 10.73GIDD248 pKa = 3.03QDD250 pKa = 4.04YY251 pKa = 8.95TFNQQGVNKK260 pKa = 9.49RR261 pKa = 11.84LRR263 pKa = 11.84KK264 pKa = 10.0GPFYY268 pKa = 11.2SLDD271 pKa = 3.42LSSATDD277 pKa = 3.69RR278 pKa = 11.84FPLVFQQKK286 pKa = 7.92VVEE289 pKa = 4.16MLTCKK294 pKa = 10.04EE295 pKa = 4.19YY296 pKa = 11.08SEE298 pKa = 4.2AWSRR302 pKa = 11.84LLVNKK307 pKa = 9.51EE308 pKa = 4.65FYY310 pKa = 10.99VPWSDD315 pKa = 3.89SFINYY320 pKa = 9.69SCGQPMGAYY329 pKa = 9.92SSWSVFTLSHH339 pKa = 6.95HH340 pKa = 4.96ITVRR344 pKa = 11.84IAALMVGIPYY354 pKa = 9.77FDD356 pKa = 3.56QYY358 pKa = 11.83AILGDD363 pKa = 4.64DD364 pKa = 3.24IVIANSLVTKK374 pKa = 9.94KK375 pKa = 10.46YY376 pKa = 10.81KK377 pKa = 10.48EE378 pKa = 4.27IISDD382 pKa = 3.87LGVSISEE389 pKa = 4.33AKK391 pKa = 10.26SHH393 pKa = 6.09EE394 pKa = 4.23SSNTYY399 pKa = 10.11EE400 pKa = 4.2FAKK403 pKa = 9.59RR404 pKa = 11.84WYY406 pKa = 8.42VNSVEE411 pKa = 4.29VTGAQINAFMTKK423 pKa = 8.08STVWSTLINEE433 pKa = 4.35YY434 pKa = 10.97KK435 pKa = 10.23EE436 pKa = 4.59ICSKK440 pKa = 8.9WQIRR444 pKa = 11.84EE445 pKa = 3.94FEE447 pKa = 4.14TEE449 pKa = 3.31ARR451 pKa = 11.84IIYY454 pKa = 10.0DD455 pKa = 3.78LFLIFFPDD463 pKa = 2.97VSEE466 pKa = 4.3AFRR469 pKa = 11.84DD470 pKa = 3.57RR471 pKa = 11.84LVRR474 pKa = 11.84KK475 pKa = 9.7ARR477 pKa = 11.84IFLSLPWRR485 pKa = 11.84RR486 pKa = 11.84EE487 pKa = 3.78GTSASEE493 pKa = 4.1QILQFVLLTNASALGCFNSSTRR515 pKa = 11.84RR516 pKa = 11.84AKK518 pKa = 10.53EE519 pKa = 3.65FFVSSMAEE527 pKa = 3.46IKK529 pKa = 10.62ARR531 pKa = 11.84EE532 pKa = 4.09LNGGLIKK539 pKa = 10.25TNGLINDD546 pKa = 5.11LIKK549 pKa = 10.63KK550 pKa = 9.59FKK552 pKa = 10.7YY553 pKa = 10.64LLINYY558 pKa = 8.02KK559 pKa = 9.99GHH561 pKa = 5.78NHH563 pKa = 6.32HH564 pKa = 6.75EE565 pKa = 4.06ALKK568 pKa = 10.31AIPFVRR574 pKa = 11.84LILDD578 pKa = 3.31QRR580 pKa = 11.84VDD582 pKa = 2.95IMNSVDD588 pKa = 3.91LLQDD592 pKa = 3.63ASALTAEE599 pKa = 5.53DD600 pKa = 3.23IVLDD604 pKa = 4.1YY605 pKa = 10.44KK606 pKa = 10.97QKK608 pKa = 11.26APISTFDD615 pKa = 3.3PTKK618 pKa = 10.22ILTKK622 pKa = 9.59RR623 pKa = 11.84THH625 pKa = 5.78EE626 pKa = 4.8LILNRR631 pKa = 11.84DD632 pKa = 3.36ATIVNQYY639 pKa = 9.97SRR641 pKa = 11.84WIKK644 pKa = 10.2DD645 pKa = 3.35YY646 pKa = 11.53NVIKK650 pKa = 10.87QNVLNNSCDD659 pKa = 3.75EE660 pKa = 4.12NTEE663 pKa = 3.96RR664 pKa = 11.84AWARR668 pKa = 11.84KK669 pKa = 8.84CFRR672 pKa = 11.84TATIGAVMPGFPLL685 pKa = 4.1

Molecular weight: 78.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2I5ARF2|A0A2I5ARF2_9VIRU RNA-dependent RNA polymerase OS=Erysiphe necator mitovirus 3 OX=2052563 PE=4 SV=1
MM1 pKa = 7.71RR2 pKa = 11.84GVDD5 pKa = 3.37NTIQRR10 pKa = 11.84IKK12 pKa = 10.65DD13 pKa = 3.57SKK15 pKa = 10.72LHH17 pKa = 5.05ITRR20 pKa = 11.84YY21 pKa = 8.23MCGQALYY28 pKa = 10.94SSINPSVGINKK39 pKa = 9.7KK40 pKa = 10.17GLPIWIGTEE49 pKa = 3.92MNKK52 pKa = 10.28LADD55 pKa = 3.66GDD57 pKa = 3.64KK58 pKa = 9.98WEE60 pKa = 4.37KK61 pKa = 10.64RR62 pKa = 11.84LLLTLLSVSRR72 pKa = 11.84ALPGSQYY79 pKa = 10.5IPPLSSLSDD88 pKa = 3.09KK89 pKa = 9.73PTYY92 pKa = 10.47KK93 pKa = 9.98EE94 pKa = 4.32DD95 pKa = 4.17KK96 pKa = 8.04ISEE99 pKa = 4.43YY100 pKa = 10.2ISYY103 pKa = 10.44IPMVMQMMNIPSKK116 pKa = 11.02INVKK120 pKa = 8.59WRR122 pKa = 11.84QFHH125 pKa = 6.12LTTKK129 pKa = 10.33KK130 pKa = 10.77GPNGQAMVSSIRR142 pKa = 11.84DD143 pKa = 3.33AHH145 pKa = 6.97LLDD148 pKa = 5.66DD149 pKa = 5.65GMLKK153 pKa = 10.47DD154 pKa = 3.51IRR156 pKa = 11.84TVAGDD161 pKa = 3.7EE162 pKa = 4.03IIEE165 pKa = 4.49VINHH169 pKa = 5.45CRR171 pKa = 11.84EE172 pKa = 3.92LDD174 pKa = 3.69PEE176 pKa = 4.34SVEE179 pKa = 4.48VVAKK183 pKa = 10.96SMVKK187 pKa = 10.41PSTATLNEE195 pKa = 4.37DD196 pKa = 3.33KK197 pKa = 11.36GMDD200 pKa = 3.59KK201 pKa = 10.82PSTLLRR207 pKa = 11.84KK208 pKa = 10.06LSIVHH213 pKa = 5.77APEE216 pKa = 4.1RR217 pKa = 11.84KK218 pKa = 9.33SRR220 pKa = 11.84IIAILDD226 pKa = 3.25YY227 pKa = 8.25WTQSSLKK234 pKa = 8.84PLHH237 pKa = 6.83DD238 pKa = 5.31RR239 pKa = 11.84IFSILKK245 pKa = 10.73GIDD248 pKa = 3.03QDD250 pKa = 4.04YY251 pKa = 8.95TFNQQGVNKK260 pKa = 9.49RR261 pKa = 11.84LRR263 pKa = 11.84KK264 pKa = 10.0GPFYY268 pKa = 11.2SLDD271 pKa = 3.42LSSATDD277 pKa = 3.69RR278 pKa = 11.84FPLVFQQKK286 pKa = 7.92VVEE289 pKa = 4.16MLTCKK294 pKa = 10.04EE295 pKa = 4.19YY296 pKa = 11.08SEE298 pKa = 4.2AWSRR302 pKa = 11.84LLVNKK307 pKa = 9.51EE308 pKa = 4.65FYY310 pKa = 10.99VPWSDD315 pKa = 3.89SFINYY320 pKa = 9.69SCGQPMGAYY329 pKa = 9.92SSWSVFTLSHH339 pKa = 6.95HH340 pKa = 4.96ITVRR344 pKa = 11.84IAALMVGIPYY354 pKa = 9.77FDD356 pKa = 3.56QYY358 pKa = 11.83AILGDD363 pKa = 4.64DD364 pKa = 3.24IVIANSLVTKK374 pKa = 9.94KK375 pKa = 10.46YY376 pKa = 10.81KK377 pKa = 10.48EE378 pKa = 4.27IISDD382 pKa = 3.87LGVSISEE389 pKa = 4.33AKK391 pKa = 10.26SHH393 pKa = 6.09EE394 pKa = 4.23SSNTYY399 pKa = 10.11EE400 pKa = 4.2FAKK403 pKa = 9.59RR404 pKa = 11.84WYY406 pKa = 8.42VNSVEE411 pKa = 4.29VTGAQINAFMTKK423 pKa = 8.08STVWSTLINEE433 pKa = 4.35YY434 pKa = 10.97KK435 pKa = 10.23EE436 pKa = 4.59ICSKK440 pKa = 8.9WQIRR444 pKa = 11.84EE445 pKa = 3.94FEE447 pKa = 4.14TEE449 pKa = 3.31ARR451 pKa = 11.84IIYY454 pKa = 10.0DD455 pKa = 3.78LFLIFFPDD463 pKa = 2.97VSEE466 pKa = 4.3AFRR469 pKa = 11.84DD470 pKa = 3.57RR471 pKa = 11.84LVRR474 pKa = 11.84KK475 pKa = 9.7ARR477 pKa = 11.84IFLSLPWRR485 pKa = 11.84RR486 pKa = 11.84EE487 pKa = 3.78GTSASEE493 pKa = 4.1QILQFVLLTNASALGCFNSSTRR515 pKa = 11.84RR516 pKa = 11.84AKK518 pKa = 10.53EE519 pKa = 3.65FFVSSMAEE527 pKa = 3.46IKK529 pKa = 10.62ARR531 pKa = 11.84EE532 pKa = 4.09LNGGLIKK539 pKa = 10.25TNGLINDD546 pKa = 5.11LIKK549 pKa = 10.63KK550 pKa = 9.59FKK552 pKa = 10.7YY553 pKa = 10.64LLINYY558 pKa = 8.02KK559 pKa = 9.99GHH561 pKa = 5.78NHH563 pKa = 6.32HH564 pKa = 6.75EE565 pKa = 4.06ALKK568 pKa = 10.31AIPFVRR574 pKa = 11.84LILDD578 pKa = 3.31QRR580 pKa = 11.84VDD582 pKa = 2.95IMNSVDD588 pKa = 3.91LLQDD592 pKa = 3.63ASALTAEE599 pKa = 5.53DD600 pKa = 3.23IVLDD604 pKa = 4.1YY605 pKa = 10.44KK606 pKa = 10.97QKK608 pKa = 11.26APISTFDD615 pKa = 3.3PTKK618 pKa = 10.22ILTKK622 pKa = 9.59RR623 pKa = 11.84THH625 pKa = 5.78EE626 pKa = 4.8LILNRR631 pKa = 11.84DD632 pKa = 3.36ATIVNQYY639 pKa = 9.97SRR641 pKa = 11.84WIKK644 pKa = 10.2DD645 pKa = 3.35YY646 pKa = 11.53NVIKK650 pKa = 10.87QNVLNNSCDD659 pKa = 3.75EE660 pKa = 4.12NTEE663 pKa = 3.96RR664 pKa = 11.84AWARR668 pKa = 11.84KK669 pKa = 8.84CFRR672 pKa = 11.84TATIGAVMPGFPLL685 pKa = 4.1

Molecular weight: 78.27 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1

0

1

685

685

685

685.0

78.27

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.985 ± 0.0

1.168 ± 0.0

5.693 ± 0.0

5.255 ± 0.0

3.942 ± 0.0

4.234 ± 0.0

1.898 ± 0.0

8.759 ± 0.0

7.591 ± 0.0

9.489 ± 0.0

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.628 ± 0.0

4.818 ± 0.0

3.796 ± 0.0

3.358 ± 0.0

5.547 ± 0.0

8.905 ± 0.0

5.401 ± 0.0

5.985 ± 0.0

1.898 ± 0.0

3.65 ± 0.0
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski