Chloroflexi bacterium

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Chloroflexi; unclassified Chloroflexi

Average proteome isoelectric point is 6.6

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1223 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A4Q3MC73|A0A4Q3MC73_9CHLR Uncharacterized protein OS=Chloroflexi bacterium OX=2026724 GN=EOO17_05140 PE=4 SV=1
MM1 pKa = 7.05TFEE4 pKa = 4.83AASAPQPVTTTPAVAPPAPKK24 pKa = 10.33AIGNIVQDD32 pKa = 3.67MLDD35 pKa = 3.86PVASAAISAYY45 pKa = 9.56NGKK48 pKa = 9.78AIPMPAQQAFDD59 pKa = 3.57GSKK62 pKa = 10.52PLDD65 pKa = 3.5VQVAEE70 pKa = 4.25TAEE73 pKa = 4.55LFQQSTVFADD83 pKa = 5.32ANACDD88 pKa = 3.65QDD90 pKa = 3.84GFNAMVACEE99 pKa = 3.73AAAVDD104 pKa = 3.94THH106 pKa = 7.49NGDD109 pKa = 3.76AVIAPITVSNRR120 pKa = 11.84FNPATVHH127 pKa = 6.24SVEE130 pKa = 4.29PLCGQLYY137 pKa = 9.98SINYY141 pKa = 9.51CEE143 pKa = 5.15GSNQTDD149 pKa = 3.92HH150 pKa = 7.21INLNRR155 pKa = 11.84TAFDD159 pKa = 3.32SLADD163 pKa = 3.77HH164 pKa = 6.58VPADD168 pKa = 4.24RR169 pKa = 11.84ADD171 pKa = 3.45LMMVILAHH179 pKa = 6.92EE180 pKa = 5.09DD181 pKa = 3.1GHH183 pKa = 7.2NQDD186 pKa = 3.53QSNGHH191 pKa = 6.1TGVDD195 pKa = 3.41TIDD198 pKa = 3.59FQEE201 pKa = 4.85LLTAALQAAA210 pKa = 4.5

Molecular weight: 22.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A4Q3MGI2|A0A4Q3MGI2_9CHLR Uncharacterized protein OS=Chloroflexi bacterium OX=2026724 GN=EOO17_01595 PE=4 SV=1
MM1 pKa = 8.07PKK3 pKa = 8.97RR4 pKa = 11.84THH6 pKa = 5.84QPHH9 pKa = 5.2TRR11 pKa = 11.84HH12 pKa = 6.54RR13 pKa = 11.84ARR15 pKa = 11.84THH17 pKa = 5.52GFRR20 pKa = 11.84ARR22 pKa = 11.84VSTKK26 pKa = 10.3AGRR29 pKa = 11.84AVIKK33 pKa = 10.13RR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84IKK38 pKa = 10.62GRR40 pKa = 11.84AKK42 pKa = 10.0LTVV45 pKa = 3.04

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1223

0

1223

316953

38

1319

259.2

28.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.123 ± 0.083

0.635 ± 0.026

6.039 ± 0.061

5.386 ± 0.067

3.713 ± 0.053

7.022 ± 0.07

1.962 ± 0.042

6.884 ± 0.059

5.51 ± 0.074

8.815 ± 0.072

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.226 ± 0.03

4.187 ± 0.056

3.966 ± 0.049

4.112 ± 0.057

5.049 ± 0.064

6.897 ± 0.072

6.636 ± 0.062

7.402 ± 0.064

1.095 ± 0.029

3.342 ± 0.045
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski