Microbacterium phage Naby

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 26 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J6TJW8|A0A5J6TJW8_9CAUD HNH endonuclease OS=Microbacterium phage Naby OX=2599856 GN=26 PE=4 SV=1
MM1 pKa = 7.74LCRR4 pKa = 11.84GCSQPAVDD12 pKa = 4.32PQDD15 pKa = 3.35VPRR18 pKa = 11.84YY19 pKa = 8.31DD20 pKa = 3.94TEE22 pKa = 4.47NPAADD27 pKa = 3.76PEE29 pKa = 4.31RR30 pKa = 11.84PQLAGPYY37 pKa = 9.69CPSCWPVTVTGRR49 pKa = 11.84LNPQEE54 pKa = 4.07EE55 pKa = 4.79SEE57 pKa = 4.24

Molecular weight: 6.27 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J6TIG4|A0A5J6TIG4_9CAUD Uncharacterized protein OS=Microbacterium phage Naby OX=2599856 GN=19 PE=4 SV=1
MM1 pKa = 7.83AEE3 pKa = 3.92YY4 pKa = 10.26QNRR7 pKa = 11.84SIRR10 pKa = 11.84IDD12 pKa = 3.35GLRR15 pKa = 11.84EE16 pKa = 3.64LNAKK20 pKa = 9.43LRR22 pKa = 11.84AAGDD26 pKa = 3.72EE27 pKa = 4.39SADD30 pKa = 4.83LPDD33 pKa = 5.54LMQQLGQLVIANARR47 pKa = 11.84VPAKK51 pKa = 10.34SGEE54 pKa = 4.09LAGTLRR60 pKa = 11.84AGRR63 pKa = 11.84GRR65 pKa = 11.84TKK67 pKa = 10.34AVVRR71 pKa = 11.84AGYY74 pKa = 10.43AKK76 pKa = 10.18RR77 pKa = 11.84GAHH80 pKa = 6.45AGVIHH85 pKa = 6.43YY86 pKa = 7.63GNPHH90 pKa = 6.44RR91 pKa = 11.84GSRR94 pKa = 11.84AQPFLVDD101 pKa = 3.78ALRR104 pKa = 11.84RR105 pKa = 11.84AQPQLVTTLAAGMDD119 pKa = 3.63ALIRR123 pKa = 11.84KK124 pKa = 8.65HH125 pKa = 6.46KK126 pKa = 10.43LL127 pKa = 3.02

Molecular weight: 13.7 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

26

0

26

5410

57

699

208.1

22.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

15.157 ± 0.612

0.518 ± 0.166

5.379 ± 0.486

5.176 ± 0.591

2.976 ± 0.268

8.983 ± 0.572

1.294 ± 0.198

4.288 ± 0.428

1.996 ± 0.274

10.166 ± 0.7

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.885 ± 0.26

2.662 ± 0.223

4.861 ± 0.409

4.344 ± 0.338

7.394 ± 0.737

5.564 ± 0.521

6.137 ± 0.552

7.209 ± 0.499

1.645 ± 0.207

2.366 ± 0.324
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski