Glarea lozoyensis (strain ATCC 74030 / MF5533)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Leotiomycetes; Helotiales; Helotiaceae; Glarea; Glarea lozoyensis

Average proteome isoelectric point is 6.39

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 7926 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|H0EPE5|H0EPE5_GLAL7 Putative AMSH-like protease sst2 OS=Glarea lozoyensis (strain ATCC 74030 / MF5533) OX=1104152 GN=M7I_4522 PE=4 SV=1
MM1 pKa = 7.7AALLDD6 pKa = 4.28HH7 pKa = 7.62DD8 pKa = 4.45SQGVDD13 pKa = 3.71GVFHH17 pKa = 6.53LQALGYY23 pKa = 9.23VSHH26 pKa = 6.91SEE28 pKa = 4.01SDD30 pKa = 3.51PDD32 pKa = 3.16ATAIFLEE39 pKa = 4.51EE40 pKa = 4.4DD41 pKa = 3.74LVPP44 pKa = 4.8

Molecular weight: 4.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|H0EHU1|H0EHU1_GLAL7 Putative General alpha-glucoside permease OS=Glarea lozoyensis (strain ATCC 74030 / MF5533) OX=1104152 GN=M7I_2080 PE=3 SV=1
MM1 pKa = 7.74AARR4 pKa = 11.84IHH6 pKa = 6.35RR7 pKa = 11.84KK8 pKa = 8.04VIKK11 pKa = 9.83MRR13 pKa = 11.84AKK15 pKa = 8.42WRR17 pKa = 11.84KK18 pKa = 8.58KK19 pKa = 8.73RR20 pKa = 11.84VRR22 pKa = 11.84RR23 pKa = 11.84LKK25 pKa = 9.95RR26 pKa = 11.84KK27 pKa = 8.83RR28 pKa = 11.84RR29 pKa = 11.84KK30 pKa = 7.46TRR32 pKa = 11.84ARR34 pKa = 11.84SKK36 pKa = 11.05

Molecular weight: 4.64 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

7926

0

7926

2715168

33

6470

342.6

37.9

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.621 ± 0.027

1.017 ± 0.011

5.892 ± 0.023

7.106 ± 0.036

3.696 ± 0.021

7.192 ± 0.03

2.112 ± 0.012

5.248 ± 0.023

6.068 ± 0.032

8.359 ± 0.035

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.323 ± 0.012

3.918 ± 0.018

5.399 ± 0.03

3.748 ± 0.021

5.47 ± 0.027

7.554 ± 0.033

5.916 ± 0.019

6.313 ± 0.023

1.341 ± 0.011

2.703 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski