Sclerophthora macrospora virus A

Taxonomy: Viruses; Riboviria; unclassified Riboviria; unclassified ssRNA viruses; unclassified ssRNA positive-strand viruses

Average proteome isoelectric point is 8.49

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q8BET0|Q8BET0_9VIRU RNA replicase OS=Sclerophthora macrospora virus A OX=191289 PE=3 SV=1
MM1 pKa = 7.29PWKK4 pKa = 9.94PGKK7 pKa = 9.97CHH9 pKa = 7.26LLAAGLAGVCCIAYY23 pKa = 7.41TKK25 pKa = 10.48RR26 pKa = 11.84GSLRR30 pKa = 11.84DD31 pKa = 3.46AASALCEE38 pKa = 4.04WLADD42 pKa = 3.89EE43 pKa = 4.61PAIVSDD49 pKa = 3.93LSRR52 pKa = 11.84DD53 pKa = 3.6AFTTTCVDD61 pKa = 3.37PVVAISGHH69 pKa = 4.23THH71 pKa = 6.69ASAASLRR78 pKa = 11.84TAATRR83 pKa = 11.84YY84 pKa = 8.07AQNVAQYY91 pKa = 9.57CGTEE95 pKa = 4.27VYY97 pKa = 10.26VVGMSRR103 pKa = 11.84SDD105 pKa = 3.13QRR107 pKa = 11.84KK108 pKa = 9.02DD109 pKa = 3.36MKK111 pKa = 10.79GSRR114 pKa = 11.84QWYY117 pKa = 4.31WTKK120 pKa = 11.1DD121 pKa = 3.23VNAANRR127 pKa = 11.84NDD129 pKa = 3.96RR130 pKa = 11.84PGHH133 pKa = 5.58RR134 pKa = 11.84DD135 pKa = 2.71IRR137 pKa = 11.84FLCDD141 pKa = 2.45VDD143 pKa = 5.16YY144 pKa = 11.08YY145 pKa = 11.83VDD147 pKa = 3.79MPAMLVAEE155 pKa = 5.23AKK157 pKa = 10.01PVLLYY162 pKa = 10.21TVVPEE167 pKa = 4.32EE168 pKa = 4.34ATSSANDD175 pKa = 3.02EE176 pKa = 4.23SAFYY180 pKa = 10.78FEE182 pKa = 5.82ADD184 pKa = 3.08GSLRR188 pKa = 11.84TMVAGGGGYY197 pKa = 7.35SHH199 pKa = 7.39CIWDD203 pKa = 3.7YY204 pKa = 11.32AADD207 pKa = 3.63SFLVVKK213 pKa = 9.17KK214 pKa = 10.28ICGVPIRR221 pKa = 11.84AVAYY225 pKa = 9.43AVEE228 pKa = 4.39RR229 pKa = 11.84KK230 pKa = 8.59QVGKK234 pKa = 9.65HH235 pKa = 4.19RR236 pKa = 11.84QMVLLAPVRR245 pKa = 11.84AFSGLAAILAYY256 pKa = 10.69LLLEE260 pKa = 4.32TKK262 pKa = 8.77EE263 pKa = 3.97LRR265 pKa = 11.84RR266 pKa = 11.84FNPIVEE272 pKa = 4.3AGGEE276 pKa = 4.03KK277 pKa = 9.88FVRR280 pKa = 11.84FNVMSSDD287 pKa = 3.32GALMVTTARR296 pKa = 11.84PGSSLCATVSCADD309 pKa = 4.13DD310 pKa = 4.23DD311 pKa = 5.66AIATVARR318 pKa = 11.84LGSTNLMLPTTASWVKK334 pKa = 10.33DD335 pKa = 3.94RR336 pKa = 11.84PAAAVLTDD344 pKa = 3.36YY345 pKa = 10.84HH346 pKa = 6.78RR347 pKa = 11.84KK348 pKa = 9.29CGKK351 pKa = 8.57KK352 pKa = 10.21AKK354 pKa = 9.67FVVYY358 pKa = 9.47PVEE361 pKa = 4.05QGVRR365 pKa = 11.84AYY367 pKa = 10.17QYY369 pKa = 11.21KK370 pKa = 10.21PDD372 pKa = 4.42EE373 pKa = 4.71FDD375 pKa = 2.84QAARR379 pKa = 11.84PKK381 pKa = 9.8LQAFMSPIVHH391 pKa = 6.5GAFAPVLNRR400 pKa = 11.84AGEE403 pKa = 4.13EE404 pKa = 3.93RR405 pKa = 11.84CVEE408 pKa = 3.86GRR410 pKa = 11.84INSLRR415 pKa = 11.84KK416 pKa = 9.65PEE418 pKa = 3.88PHH420 pKa = 6.95PSNFRR425 pKa = 11.84DD426 pKa = 3.28RR427 pKa = 11.84CIDD430 pKa = 3.4EE431 pKa = 4.59FADD434 pKa = 3.92LVVSGMLLEE443 pKa = 4.38PVCFEE448 pKa = 4.32VVNSKK453 pKa = 7.44QTRR456 pKa = 11.84STQKK460 pKa = 10.64LSIAKK465 pKa = 9.8AVLTGKK471 pKa = 9.85FRR473 pKa = 11.84QAVLKK478 pKa = 10.86CFGKK482 pKa = 10.66AEE484 pKa = 4.58AYY486 pKa = 9.68PDD488 pKa = 3.53VKK490 pKa = 10.81DD491 pKa = 3.85PRR493 pKa = 11.84NISQYY498 pKa = 11.4NDD500 pKa = 3.26ADD502 pKa = 4.14KK503 pKa = 11.63LDD505 pKa = 3.67MATFALALSEE515 pKa = 4.67HH516 pKa = 6.08MKK518 pKa = 9.62QFKK521 pKa = 9.69WYY523 pKa = 10.11GPGKK527 pKa = 8.81TPLEE531 pKa = 3.85IANRR535 pKa = 11.84VVEE538 pKa = 4.43ICQAADD544 pKa = 3.41FVNISDD550 pKa = 3.79YY551 pKa = 11.35HH552 pKa = 8.03RR553 pKa = 11.84MDD555 pKa = 3.0GTITYY560 pKa = 7.32TLRR563 pKa = 11.84RR564 pKa = 11.84VEE566 pKa = 4.33RR567 pKa = 11.84AVCMKK572 pKa = 10.8AFANHH577 pKa = 5.83SACLNEE583 pKa = 3.97LLKK586 pKa = 11.17RR587 pKa = 11.84NVDD590 pKa = 3.34NVGYY594 pKa = 9.55LPHH597 pKa = 6.74GTTFNQGSSHH607 pKa = 6.79GSGCSATSLFQTLRR621 pKa = 11.84AAFNAYY627 pKa = 10.22LGFRR631 pKa = 11.84HH632 pKa = 5.86TRR634 pKa = 11.84NQAGRR639 pKa = 11.84MFSPKK644 pKa = 9.86EE645 pKa = 3.72AFASLGIHH653 pKa = 6.81LGDD656 pKa = 5.87DD657 pKa = 3.98GLDD660 pKa = 3.49GNLPIGSHH668 pKa = 4.91QWASKK673 pKa = 7.08QTGLILEE680 pKa = 4.38AALVYY685 pKa = 10.21RR686 pKa = 11.84GYY688 pKa = 10.82RR689 pKa = 11.84GVNFLARR696 pKa = 11.84YY697 pKa = 7.47YY698 pKa = 10.9SPEE701 pKa = 3.69VWQGCPDD708 pKa = 3.63SMCDD712 pKa = 4.07FKK714 pKa = 11.42RR715 pKa = 11.84QISKK719 pKa = 9.63FHH721 pKa = 5.25TTVRR725 pKa = 11.84LPSNVTPEE733 pKa = 3.87QKK735 pKa = 10.43LVEE738 pKa = 4.08KK739 pKa = 10.74SMSYY743 pKa = 10.74VATDD747 pKa = 3.02GNTPVIGQLCKK758 pKa = 10.16RR759 pKa = 11.84VLLLSSLRR767 pKa = 11.84PRR769 pKa = 11.84TLFGLGNWWSQFDD782 pKa = 5.25DD783 pKa = 4.0SVQFPNNNVGGWMDD797 pKa = 3.73VEE799 pKa = 4.73IDD801 pKa = 3.55HH802 pKa = 6.44QFPEE806 pKa = 4.27FDD808 pKa = 4.17RR809 pKa = 11.84NLFDD813 pKa = 3.38KK814 pKa = 10.63WLDD817 pKa = 3.71SVQKK821 pKa = 11.19AEE823 pKa = 4.9DD824 pKa = 3.93LLSAPLCAEE833 pKa = 4.92PKK835 pKa = 10.07PPTPGCVAVVVDD847 pKa = 4.85EE848 pKa = 4.94EE849 pKa = 4.71IIPPRR854 pKa = 11.84QDD856 pKa = 2.98VEE858 pKa = 4.28PARR861 pKa = 11.84SSSPPRR867 pKa = 11.84RR868 pKa = 11.84TPLRR872 pKa = 11.84PTRR875 pKa = 11.84YY876 pKa = 8.22KK877 pKa = 9.89VTVARR882 pKa = 11.84EE883 pKa = 3.81KK884 pKa = 10.51ATLLNPPKK892 pKa = 9.87PRR894 pKa = 11.84RR895 pKa = 11.84ATAA898 pKa = 3.35

Molecular weight: 99.37 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|Q8BET0|Q8BET0_9VIRU RNA replicase OS=Sclerophthora macrospora virus A OX=191289 PE=3 SV=1
MM1 pKa = 7.52NYY3 pKa = 10.16SSGMSTTSGTSPTGRR18 pKa = 11.84HH19 pKa = 6.01LIKK22 pKa = 10.33EE23 pKa = 4.24VRR25 pKa = 11.84TDD27 pKa = 3.41QAVQQPASSKK37 pKa = 9.96PSEE40 pKa = 4.1LRR42 pKa = 11.84LTHH45 pKa = 6.74ILDD48 pKa = 3.62SGTRR52 pKa = 11.84GTKK55 pKa = 9.93RR56 pKa = 11.84AEE58 pKa = 4.04CSPLRR63 pKa = 11.84RR64 pKa = 11.84PLQVLASISVTTVSMVTYY82 pKa = 9.99PSDD85 pKa = 3.56LTSGRR90 pKa = 11.84QNRR93 pKa = 11.84QGSYY97 pKa = 7.2WKK99 pKa = 10.6RR100 pKa = 11.84PWCTEE105 pKa = 4.02AIEE108 pKa = 5.14EE109 pKa = 4.52SISWHH114 pKa = 4.63ATIRR118 pKa = 11.84PKK120 pKa = 10.52SGKK123 pKa = 9.88GVLIVCVTSRR133 pKa = 11.84DD134 pKa = 3.46RR135 pKa = 11.84SRR137 pKa = 11.84SSIQRR142 pKa = 11.84FAYY145 pKa = 10.72LLTSRR150 pKa = 11.84LNKK153 pKa = 9.26SWSRR157 pKa = 11.84NPCPMWQPMEE167 pKa = 3.98IHH169 pKa = 6.64PLSVSFARR177 pKa = 11.84EE178 pKa = 4.24CFCSHH183 pKa = 6.99PCAPEE188 pKa = 4.14LFLDD192 pKa = 4.08SVIGGLSSTIPYY204 pKa = 9.96SSPTIMLEE212 pKa = 4.58DD213 pKa = 2.93GWMWKK218 pKa = 10.41LIISSQNSTEE228 pKa = 3.91ISSTSGWIQFKK239 pKa = 10.46KK240 pKa = 10.48RR241 pKa = 11.84RR242 pKa = 11.84TCFQLHH248 pKa = 5.34YY249 pKa = 10.2VLNPSHH255 pKa = 5.94QHH257 pKa = 6.68LDD259 pKa = 3.56ALQSWLTRR267 pKa = 11.84RR268 pKa = 11.84LSLHH272 pKa = 6.87DD273 pKa = 3.89RR274 pKa = 11.84MLSQHH279 pKa = 5.5EE280 pKa = 3.95ALRR283 pKa = 11.84RR284 pKa = 11.84LGEE287 pKa = 3.91HH288 pKa = 6.64RR289 pKa = 4.27

Molecular weight: 32.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3

0

3

1609

289

898

536.3

58.97

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.136 ± 1.968

2.362 ± 0.488

4.475 ± 1.081

4.102 ± 0.52

3.543 ± 0.569

6.712 ± 1.085

2.113 ± 0.73

3.853 ± 0.722

4.537 ± 0.545

8.515 ± 0.432

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.362 ± 0.152

3.915 ± 0.921

5.718 ± 0.209

3.729 ± 0.421

6.464 ± 1.408

9.012 ± 2.41

6.401 ± 0.882

8.142 ± 1.629

1.678 ± 0.541

3.232 ± 0.455
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski