Hwanghaeella grinnelliae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rhodospirillales; Rhodospirillaceae; Hwanghaeella

Average proteome isoelectric point is 5.91

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4801 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A3S2WCE6|A0A3S2WCE6_9PROT FAD-binding oxidoreductase OS=Hwanghaeella grinnelliae OX=2500179 GN=EOI86_08305 PE=4 SV=1
MM1 pKa = 7.56AAGFALGFFACQAQACGPDD20 pKa = 3.23TRR22 pKa = 11.84VQFTEE27 pKa = 4.47GSDD30 pKa = 3.39DD31 pKa = 3.57TFLIEE36 pKa = 4.1NNSVIGLSLQQMAIDD51 pKa = 4.86LSSSAGGVIFDD62 pKa = 4.36TDD64 pKa = 3.48YY65 pKa = 12.01GGAGWSAYY73 pKa = 8.67QPFQPLASTAVLRR86 pKa = 11.84AASSGLDD93 pKa = 3.54GYY95 pKa = 9.9QQLSLVFDD103 pKa = 4.12RR104 pKa = 11.84FARR107 pKa = 11.84GDD109 pKa = 3.59SFAFEE114 pKa = 4.68IDD116 pKa = 5.61LDD118 pKa = 4.13DD119 pKa = 3.86QLQDD123 pKa = 3.43SDD125 pKa = 3.74HH126 pKa = 6.44GQTIVSGTEE135 pKa = 3.5IAGGAVMGVFTDD147 pKa = 3.36KK148 pKa = 10.94HH149 pKa = 5.23GQTYY153 pKa = 9.98RR154 pKa = 11.84ATGYY158 pKa = 10.82FDD160 pKa = 4.02TDD162 pKa = 3.06GQAILTQGACSS173 pKa = 3.28

Molecular weight: 18.17 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A3S2W4N9|A0A3S2W4N9_9PROT TRAP transporter large permease OS=Hwanghaeella grinnelliae OX=2500179 GN=EOI86_14520 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSRR9 pKa = 11.84LVRR12 pKa = 11.84KK13 pKa = 8.99RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.7GFRR19 pKa = 11.84SRR21 pKa = 11.84MATSSGRR28 pKa = 11.84RR29 pKa = 11.84VLANRR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.85GRR39 pKa = 11.84KK40 pKa = 8.87RR41 pKa = 11.84LSAA44 pKa = 3.96

Molecular weight: 5.23 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4801

0

4801

1580863

30

3251

329.3

35.82

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.359 ± 0.041

0.918 ± 0.011

6.309 ± 0.036

6.05 ± 0.03

3.926 ± 0.02

8.646 ± 0.038

2.007 ± 0.017

5.39 ± 0.024

3.668 ± 0.028

9.805 ± 0.037

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.668 ± 0.019

2.867 ± 0.02

4.967 ± 0.025

3.077 ± 0.018

6.28 ± 0.034

5.486 ± 0.023

5.47 ± 0.024

7.48 ± 0.025

1.298 ± 0.015

2.33 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski