Pseudomonas putida CSV86

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas; Pseudomonas putida group; Pseudomonas putida

Average proteome isoelectric point is 6.44

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5175 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|L1M4K6|L1M4K6_PSEPU Outer membrane lipoprotein carrier protein LolA OS=Pseudomonas putida CSV86 OX=1005395 GN=CSV86_027750 PE=4 SV=1
MM1 pKa = 7.58HH2 pKa = 7.86DD3 pKa = 3.85EE4 pKa = 5.17DD5 pKa = 4.39EE6 pKa = 4.39LHH8 pKa = 6.66EE9 pKa = 4.8PEE11 pKa = 5.34HH12 pKa = 6.96DD13 pKa = 3.38HH14 pKa = 7.69LLDD17 pKa = 4.63HH18 pKa = 7.0EE19 pKa = 4.59FHH21 pKa = 6.77DD22 pKa = 4.47HH23 pKa = 7.09EE24 pKa = 4.89EE25 pKa = 4.99GEE27 pKa = 4.54AYY29 pKa = 10.12ADD31 pKa = 3.77PMFDD35 pKa = 5.55DD36 pKa = 4.02VDD38 pKa = 4.27DD39 pKa = 4.91DD40 pKa = 4.17EE41 pKa = 6.1EE42 pKa = 5.53YY43 pKa = 11.43GLDD46 pKa = 3.55VLDD49 pKa = 5.94DD50 pKa = 4.33EE51 pKa = 5.4DD52 pKa = 6.27HH53 pKa = 7.21EE54 pKa = 4.97DD55 pKa = 4.36HH56 pKa = 7.55LDD58 pKa = 3.71DD59 pKa = 4.88

Molecular weight: 7.08 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|L1LY78|L1LY78_PSEPU Uncharacterized protein OS=Pseudomonas putida CSV86 OX=1005395 GN=CSV86_011600 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.56RR3 pKa = 11.84TFQPSTIKK11 pKa = 10.52RR12 pKa = 11.84ARR14 pKa = 11.84THH16 pKa = 5.94GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.16NGRR28 pKa = 11.84AVLSRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 9.94GRR39 pKa = 11.84KK40 pKa = 8.66RR41 pKa = 11.84LAVV44 pKa = 3.41

Molecular weight: 5.12 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5175

0

5175

1631647

23

6174

315.3

34.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.155 ± 0.044

0.984 ± 0.012

5.424 ± 0.027

5.765 ± 0.032

3.574 ± 0.024

8.099 ± 0.032

2.272 ± 0.019

4.627 ± 0.025

3.323 ± 0.031

11.758 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.248 ± 0.017

2.989 ± 0.026

4.844 ± 0.025

4.514 ± 0.028

6.786 ± 0.03

5.78 ± 0.032

4.758 ± 0.026

7.111 ± 0.028

1.463 ± 0.014

2.527 ± 0.019
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski