Lucheng Rn rat coronavirus

Taxonomy: Viruses; Riboviria; Orthornavirae; Pisuviricota; Pisoniviricetes; Nidovirales; Cornidovirineae; Coronaviridae; Orthocoronavirinae; Alphacoronavirus; Luchacovirus

Average proteome isoelectric point is 6.82

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L7HJ82|A0A1L7HJ82_9ALPC Spike glycoprotein OS=Lucheng Rn rat coronavirus OX=1508224 PE=4 SV=1
MM1 pKa = 7.39YY2 pKa = 10.37FFLLLLFVSADD13 pKa = 3.48AAIQTCPAPGNVNLDD28 pKa = 3.08ISKK31 pKa = 10.61LYY33 pKa = 10.88YY34 pKa = 9.2GTQASINATFQVVQVLPQVPWKK56 pKa = 10.45CNSYY60 pKa = 11.66SNGPSNKK67 pKa = 9.24FNGIGVFVDD76 pKa = 4.33LASAQHH82 pKa = 5.57SWHH85 pKa = 6.3LFVYY89 pKa = 10.33PSMPTNKK96 pKa = 8.32TWILSWADD104 pKa = 3.06THH106 pKa = 6.73TSFEE110 pKa = 4.36HH111 pKa = 6.53GSVISYY117 pKa = 8.02VQICKK122 pKa = 10.34YY123 pKa = 9.0PSNVVTDD130 pKa = 3.52ITNGNGCHH138 pKa = 6.14TNMAGPGATTCDD150 pKa = 3.88VILSSPLEE158 pKa = 4.14CVLNRR163 pKa = 11.84TYY165 pKa = 10.65SQQYY169 pKa = 10.18AGVSYY174 pKa = 8.6ITWYY178 pKa = 9.76NDD180 pKa = 3.4HH181 pKa = 7.25IIASIQGEE189 pKa = 4.73VFTFDD194 pKa = 3.48IGEE197 pKa = 4.11VLQWSNFSAFCGTGNKK213 pKa = 10.04CGFSYY218 pKa = 9.67ATTLSEE224 pKa = 3.78WLVRR228 pKa = 11.84TDD230 pKa = 3.58NDD232 pKa = 3.71GTVIDD237 pKa = 4.82YY238 pKa = 7.72VICDD242 pKa = 3.76TDD244 pKa = 4.17FEE246 pKa = 4.75SQLKK250 pKa = 8.56CKK252 pKa = 10.72NMVFEE257 pKa = 4.74LTPAVYY263 pKa = 10.25SGSAVEE269 pKa = 4.29LQSAIYY275 pKa = 9.56YY276 pKa = 9.33VSNEE280 pKa = 4.29LPDD283 pKa = 4.28CDD285 pKa = 5.55FSFADD290 pKa = 3.78MFMDD294 pKa = 4.14GTGNFEE300 pKa = 3.64GLRR303 pKa = 11.84RR304 pKa = 11.84HH305 pKa = 5.69VFSNCWVNYY314 pKa = 6.46TAWFACADD322 pKa = 4.31DD323 pKa = 4.03YY324 pKa = 12.04SCIIFNAIFAEE335 pKa = 4.2VRR337 pKa = 11.84YY338 pKa = 10.12KK339 pKa = 10.88LSQPDD344 pKa = 3.63GLVNPFIKK352 pKa = 10.71CNGLDD357 pKa = 3.78LYY359 pKa = 11.0SITKK363 pKa = 8.93GCSSGFVLRR372 pKa = 11.84YY373 pKa = 9.33QLYY376 pKa = 9.87ANGSFDD382 pKa = 4.02VNSYY386 pKa = 9.25TPDD389 pKa = 3.21YY390 pKa = 9.16MEE392 pKa = 4.66CFGYY396 pKa = 9.87FALYY400 pKa = 9.58NGYY403 pKa = 10.4VIYY406 pKa = 10.4NAKK409 pKa = 9.93FVSKK413 pKa = 10.42GLTVCVVQPVEE424 pKa = 4.53PEE426 pKa = 3.77LDD428 pKa = 3.41VCKK431 pKa = 10.46SYY433 pKa = 10.68TIDD436 pKa = 3.13GVTFQGILRR445 pKa = 11.84NTTHH449 pKa = 6.99QIDD452 pKa = 3.85SFHH455 pKa = 6.93NILYY459 pKa = 9.35YY460 pKa = 10.98GDD462 pKa = 3.43MVSYY466 pKa = 10.32VRR468 pKa = 11.84IRR470 pKa = 11.84GVVYY474 pKa = 10.34AVEE477 pKa = 4.24SCNRR481 pKa = 11.84FYY483 pKa = 11.67YY484 pKa = 10.69SVFKK488 pKa = 9.96TLSAIGYY495 pKa = 8.24LYY497 pKa = 10.93SGATCDD503 pKa = 3.72STDD506 pKa = 2.76VTTFMTKK513 pKa = 9.79ARR515 pKa = 11.84ATTFVDD521 pKa = 3.31SSLGCFIDD529 pKa = 4.58VSVTDD534 pKa = 4.99GNYY537 pKa = 9.43TDD539 pKa = 4.64CLNPIGNGFCVDD551 pKa = 3.72VNVTGQPVVGNIFIQTHH568 pKa = 5.14DD569 pKa = 3.41TDD571 pKa = 3.78YY572 pKa = 11.58ARR574 pKa = 11.84PILTAQQIEE583 pKa = 4.61LPIDD587 pKa = 3.45HH588 pKa = 6.39YY589 pKa = 11.93VSVKK593 pKa = 9.75EE594 pKa = 4.06QFIQTSTPKK603 pKa = 10.22FDD605 pKa = 3.87VDD607 pKa = 3.72CEE609 pKa = 4.22RR610 pKa = 11.84YY611 pKa = 9.46ICDD614 pKa = 3.08VSSDD618 pKa = 3.63CRR620 pKa = 11.84EE621 pKa = 4.04LLVKK625 pKa = 10.85YY626 pKa = 10.33GGYY629 pKa = 9.55CSKK632 pKa = 10.48ILADD636 pKa = 3.97IKK638 pKa = 10.99SSSIQLDD645 pKa = 3.72YY646 pKa = 11.29QILGLYY652 pKa = 7.6KK653 pKa = 10.11TLAVDD658 pKa = 4.82FKK660 pKa = 11.66VPDD663 pKa = 3.36IDD665 pKa = 5.12FGDD668 pKa = 4.56FNFSMYY674 pKa = 9.85MSEE677 pKa = 4.07ANGRR681 pKa = 11.84SFIEE685 pKa = 4.8DD686 pKa = 3.74LLFDD690 pKa = 5.23KK691 pKa = 10.72IVTTGPGFYY700 pKa = 9.77QDD702 pKa = 5.25YY703 pKa = 10.36YY704 pKa = 11.41DD705 pKa = 4.07CKK707 pKa = 11.08KK708 pKa = 10.59MNLQDD713 pKa = 4.03LTCKK717 pKa = 10.15QYY719 pKa = 11.27YY720 pKa = 8.62NGIMVIPPVMDD731 pKa = 3.47DD732 pKa = 3.41TLITFWSSAVAGSMTAGLFGGQAGMVSWTVALAGRR767 pKa = 11.84LNALGVMQDD776 pKa = 3.55ALVEE780 pKa = 4.24DD781 pKa = 4.41VNKK784 pKa = 10.3LANGFNNLTQYY795 pKa = 10.95VSDD798 pKa = 3.99GFKK801 pKa = 8.42TTSQALSTIQAVVNNNAQQVSQLVQGLSEE830 pKa = 4.2NFGAISNNFALIAEE844 pKa = 4.1RR845 pKa = 11.84LEE847 pKa = 4.47RR848 pKa = 11.84IEE850 pKa = 5.82AAMQMDD856 pKa = 4.41RR857 pKa = 11.84LINGRR862 pKa = 11.84MNILQNFVTNYY873 pKa = 9.51KK874 pKa = 10.59LSISEE879 pKa = 4.69LKK881 pKa = 10.33SQQALAQSLINEE893 pKa = 4.31CVYY896 pKa = 10.93AQSSRR901 pKa = 11.84NGFCGDD907 pKa = 3.51GLHH910 pKa = 6.68LFSLMQRR917 pKa = 11.84APDD920 pKa = 4.2GIMFFHH926 pKa = 6.52YY927 pKa = 9.1TLKK930 pKa = 10.61PNNTIIVEE938 pKa = 4.32TTPGLCLSNDD948 pKa = 3.04VCIAPKK954 pKa = 10.5DD955 pKa = 3.73GLFVRR960 pKa = 11.84LSTARR965 pKa = 11.84DD966 pKa = 3.78TDD968 pKa = 3.13WHH970 pKa = 5.32FTTRR974 pKa = 11.84NRR976 pKa = 11.84YY977 pKa = 8.21SPEE980 pKa = 4.89PITVNNTLTISGGVNFTVVNSTIDD1004 pKa = 4.17GIEE1007 pKa = 4.13PPANPSFDD1015 pKa = 3.75EE1016 pKa = 4.55EE1017 pKa = 4.15FAEE1020 pKa = 4.83LYY1022 pKa = 11.03KK1023 pKa = 11.06NVTLEE1028 pKa = 4.5LEE1030 pKa = 4.02QLKK1033 pKa = 10.79NISFDD1038 pKa = 3.78PEE1040 pKa = 4.25MLNLTYY1046 pKa = 10.84YY1047 pKa = 10.11IDD1049 pKa = 4.39RR1050 pKa = 11.84LDD1052 pKa = 3.88EE1053 pKa = 4.2LATNVSQLHH1062 pKa = 5.6VDD1064 pKa = 3.09VSEE1067 pKa = 4.22FNKK1070 pKa = 9.68FVQYY1074 pKa = 10.55IKK1076 pKa = 10.02WPWYY1080 pKa = 7.76VWLAIFLVLVLFSFLMLWCCCATGCCGCCGLCGAACNGCCTKK1122 pKa = 10.18PQPIEE1127 pKa = 3.79FEE1129 pKa = 4.31KK1130 pKa = 11.18VHH1132 pKa = 5.12VQQ1134 pKa = 3.25

Molecular weight: 126.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1L7HJ65|A0A1L7HJ65_9ALPC Membrane protein OS=Lucheng Rn rat coronavirus OX=1508224 GN=M PE=3 SV=1
MM1 pKa = 7.45SSNVSWADD9 pKa = 3.15QVEE12 pKa = 4.7TVNRR16 pKa = 11.84RR17 pKa = 11.84QRR19 pKa = 11.84SRR21 pKa = 11.84SRR23 pKa = 11.84GRR25 pKa = 11.84SQNRR29 pKa = 11.84TNASNPLSWFTSIIDD44 pKa = 3.59EE45 pKa = 4.55SNGNFISLMPHH56 pKa = 5.84SGVPTGMGTAAQQCGYY72 pKa = 8.99WYY74 pKa = 9.86RR75 pKa = 11.84APTVYY80 pKa = 9.83QVRR83 pKa = 11.84RR84 pKa = 11.84GKK86 pKa = 10.02RR87 pKa = 11.84VPLPPVWYY95 pKa = 8.96FYY97 pKa = 11.33FLGTGPHH104 pKa = 6.53ANAAYY109 pKa = 7.11GTAMDD114 pKa = 3.85GVFWVKK120 pKa = 9.53TKK122 pKa = 10.78NGQIDD127 pKa = 3.96PKK129 pKa = 9.07STKK132 pKa = 10.51ALGVRR137 pKa = 11.84DD138 pKa = 3.77SGTDD142 pKa = 3.25PRR144 pKa = 11.84RR145 pKa = 11.84ANIPNLPEE153 pKa = 3.99GLRR156 pKa = 11.84VNVPNASRR164 pKa = 11.84PQSRR168 pKa = 11.84AQSQTRR174 pKa = 11.84SQNTSRR180 pKa = 11.84ASSVSRR186 pKa = 11.84NGSRR190 pKa = 11.84APSVDD195 pKa = 3.03RR196 pKa = 11.84TKK198 pKa = 11.28EE199 pKa = 3.74DD200 pKa = 3.04LKK202 pKa = 11.41AVVSQLLAEE211 pKa = 4.39MGISKK216 pKa = 10.34NSKK219 pKa = 7.23QTQNQKK225 pKa = 9.11KK226 pKa = 9.71QKK228 pKa = 9.96GSTPAATPHH237 pKa = 6.75PNQDD241 pKa = 3.55GKK243 pKa = 9.81PVWKK247 pKa = 10.25KK248 pKa = 10.68KK249 pKa = 9.19PNKK252 pKa = 9.71EE253 pKa = 3.8EE254 pKa = 3.86TVAQCFGPRR263 pKa = 11.84SDD265 pKa = 3.88NKK267 pKa = 10.55NFGDD271 pKa = 4.76AEE273 pKa = 4.06FLRR276 pKa = 11.84LGVDD280 pKa = 3.38DD281 pKa = 5.19PRR283 pKa = 11.84FKK285 pKa = 10.14TASYY289 pKa = 8.67YY290 pKa = 11.24APGAAASLFDD300 pKa = 4.33SMVTVSDD307 pKa = 4.08GPDD310 pKa = 2.93GKK312 pKa = 10.96KK313 pKa = 9.72RR314 pKa = 11.84VTFHH318 pKa = 5.24TTIEE322 pKa = 4.02VDD324 pKa = 3.14PTKK327 pKa = 10.65PGFEE331 pKa = 4.09VFLAQIDD338 pKa = 4.16AFKK341 pKa = 11.0KK342 pKa = 9.97PATFQQTQNFWEE354 pKa = 4.47NQASTQNNISEE365 pKa = 4.68YY366 pKa = 10.63FRR368 pKa = 11.84GTTPGAGGSAVEE380 pKa = 4.19IEE382 pKa = 4.46TFEE385 pKa = 4.26MTDD388 pKa = 3.2EE389 pKa = 4.43TNN391 pKa = 3.0

Molecular weight: 43.07 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5

0

5

8600

78

6749

1720.0

192.39

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.86 ± 0.499

3.674 ± 0.739

6.047 ± 0.958

3.837 ± 0.266

5.895 ± 0.305

6.547 ± 0.268

1.884 ± 0.332

4.86 ± 0.729

5.419 ± 0.828

8.488 ± 0.977

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.174 ± 0.318

5.605 ± 0.445

3.442 ± 0.694

3.058 ± 0.808

3.605 ± 0.589

7.186 ± 0.662

6.035 ± 0.757

8.895 ± 0.568

1.36 ± 0.443

5.128 ± 0.496
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski