Thalassiosira pseudonana (Marine diatom) (Cyclotella nana)

Taxonomy: cellular organisms; Eukaryota; Sar; Stramenopiles; Ochrophyta; Bacillariophyta; Coscinodiscophyceae; Thalassiosirophycidae; Thalassiosirales; Thalassiosiraceae; Thalassiosira

Average proteome isoelectric point is 6.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11718 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B8CAV6|B8CAV6_THAPS Uncharacterized protein OS=Thalassiosira pseudonana OX=35128 GN=THAPSDRAFT_9279 PE=4 SV=1
MM1 pKa = 7.49KK2 pKa = 9.53PLSFTYY8 pKa = 9.92IVTMSPFLFTKK19 pKa = 10.45GASSSSARR27 pKa = 11.84TTVIRR32 pKa = 11.84RR33 pKa = 11.84MRR35 pKa = 11.84TTTTEE40 pKa = 3.69QVYY43 pKa = 10.04KK44 pKa = 10.37SSKK47 pKa = 10.14AADD50 pKa = 3.76SKK52 pKa = 11.5SSSKK56 pKa = 10.69DD57 pKa = 3.0RR58 pKa = 11.84SLLTNEE64 pKa = 4.2EE65 pKa = 4.18GAISFSFSLHH75 pKa = 5.94MPPILPPDD83 pKa = 4.4IISNNPFDD91 pKa = 5.09TIPDD95 pKa = 3.85TTTTTTTDD103 pKa = 3.13NEE105 pKa = 4.93SPFSITNDD113 pKa = 3.4GVPSLIDD120 pKa = 3.41GSMSIMDD127 pKa = 3.66TTEE130 pKa = 3.35ISAEE134 pKa = 4.06SDD136 pKa = 3.21TTPDD140 pKa = 4.05DD141 pKa = 3.69NPFDD145 pKa = 4.03VDD147 pKa = 3.79LSTRR151 pKa = 11.84VPLSTIPTLSPMEE164 pKa = 5.04DD165 pKa = 3.59DD166 pKa = 4.43NNNILTPSPSVDD178 pKa = 3.34PFSGLWDD185 pKa = 3.99LDD187 pKa = 3.49IPGAFSMSMSLSMMSMSVGYY207 pKa = 9.84FDD209 pKa = 5.32HH210 pKa = 7.7SEE212 pKa = 4.06GLPDD216 pKa = 4.92FPFSDD221 pKa = 4.08FSMPMMSMSMDD232 pKa = 5.18DD233 pKa = 4.14IVSMACPSSCTTSFCEE249 pKa = 3.8CTYY252 pKa = 10.01MEE254 pKa = 4.86EE255 pKa = 4.39VEE257 pKa = 4.77ADD259 pKa = 4.52CIPQIVNEE267 pKa = 4.31CSITQPKK274 pKa = 8.59SQCLVDD280 pKa = 5.07LFDD283 pKa = 4.32NGVGEE288 pKa = 4.84SSDD291 pKa = 3.38IFCNSAVCLGEE302 pKa = 4.16GKK304 pKa = 9.96SEE306 pKa = 4.11LEE308 pKa = 3.56CRR310 pKa = 11.84YY311 pKa = 10.3RR312 pKa = 11.84GVCFAMEE319 pKa = 4.64EE320 pKa = 4.04EE321 pKa = 5.04GGWNTIIGLSLSMSFSMSVSLTEE344 pKa = 4.12TTVTTVVPSPSFGGLTMIPTSCYY367 pKa = 10.33TFDD370 pKa = 3.97SDD372 pKa = 4.71

Molecular weight: 40.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|B5YLN3|B5YLN3_THAPS Aminopeptidase OS=Thalassiosira pseudonana OX=35128 GN=THAPS_269937 PE=3 SV=1
MM1 pKa = 7.5TKK3 pKa = 9.06RR4 pKa = 11.84TLSNKK9 pKa = 7.81TRR11 pKa = 11.84SSILKK16 pKa = 9.43VSGFRR21 pKa = 11.84ARR23 pKa = 11.84MATAQGRR30 pKa = 11.84KK31 pKa = 8.94IIRR34 pKa = 11.84TRR36 pKa = 11.84RR37 pKa = 11.84QKK39 pKa = 10.56GRR41 pKa = 11.84KK42 pKa = 8.67KK43 pKa = 10.47LAIPRR48 pKa = 3.95

Molecular weight: 5.55 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11717

1

11718

5824029

29

8463

497.0

54.81

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.707 ± 0.019

1.618 ± 0.01

6.285 ± 0.019

6.557 ± 0.022

3.487 ± 0.017

6.957 ± 0.023

2.315 ± 0.011

4.716 ± 0.017

5.367 ± 0.024

8.461 ± 0.026

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.512 ± 0.01

4.55 ± 0.015

4.743 ± 0.022

3.783 ± 0.017

5.332 ± 0.024

9.382 ± 0.033

6.009 ± 0.016

6.429 ± 0.017

1.115 ± 0.007

2.676 ± 0.011
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski