Jeotgalibacillus malaysiensis

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Planococcaceae; Jeotgalibacillus

Average proteome isoelectric point is 6.05

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4426 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A0B5AN96|A0A0B5AN96_9BACL D-alanine--D-alanine ligase OS=Jeotgalibacillus malaysiensis OX=1508404 GN=ddl PE=3 SV=1
MM1 pKa = 7.62KK2 pKa = 10.43KK3 pKa = 10.5LLFPVIAGALVLGACGSEE21 pKa = 4.09EE22 pKa = 3.99NAEE25 pKa = 4.33TTTGSAAEE33 pKa = 4.19KK34 pKa = 10.37EE35 pKa = 4.5EE36 pKa = 3.97MTISHH41 pKa = 7.6LLGDD45 pKa = 4.0TTVEE49 pKa = 3.99KK50 pKa = 10.75SPEE53 pKa = 4.16SVVVFDD59 pKa = 6.09FGTLDD64 pKa = 3.28TLDD67 pKa = 3.79ALGVEE72 pKa = 4.87VAGVPQTNIPSYY84 pKa = 8.87LSKK87 pKa = 11.5YY88 pKa = 9.63EE89 pKa = 4.14GEE91 pKa = 4.5DD92 pKa = 3.53YY93 pKa = 11.42EE94 pKa = 4.44NAGGLKK100 pKa = 10.32EE101 pKa = 4.4PDD103 pKa = 3.37FEE105 pKa = 7.27AINAMDD111 pKa = 5.57PDD113 pKa = 5.02LIIISGRR120 pKa = 11.84QADD123 pKa = 4.15LYY125 pKa = 11.36EE126 pKa = 4.47EE127 pKa = 4.73FEE129 pKa = 5.17AIAPTIYY136 pKa = 10.02MGVDD140 pKa = 3.02TEE142 pKa = 4.95NYY144 pKa = 8.7MEE146 pKa = 4.32SFEE149 pKa = 5.12EE150 pKa = 4.32NVNTLAALFEE160 pKa = 4.41KK161 pKa = 10.8EE162 pKa = 3.5EE163 pKa = 3.99AAAEE167 pKa = 4.03KK168 pKa = 10.42LDD170 pKa = 4.62EE171 pKa = 4.8INVSIDD177 pKa = 3.38EE178 pKa = 4.56LNEE181 pKa = 3.9QVTSQDD187 pKa = 3.1LEE189 pKa = 4.32ALVTLANDD197 pKa = 4.45GKK199 pKa = 10.57ISAYY203 pKa = 10.24GAGSRR208 pKa = 11.84FGIIHH213 pKa = 7.04DD214 pKa = 3.85VFGFTPADD222 pKa = 3.48EE223 pKa = 4.69TIEE226 pKa = 4.17VSTHH230 pKa = 4.87GQSISYY236 pKa = 9.54EE237 pKa = 4.28YY238 pKa = 10.19IVEE241 pKa = 4.33EE242 pKa = 4.28DD243 pKa = 3.25PQYY246 pKa = 11.3IFVVDD251 pKa = 4.52RR252 pKa = 11.84GAAVDD257 pKa = 4.24GEE259 pKa = 4.54SSAKK263 pKa = 9.74QVVEE267 pKa = 3.91NEE269 pKa = 4.5FIEE272 pKa = 4.78GTQAYY277 pKa = 8.5EE278 pKa = 3.94NGNIIYY284 pKa = 10.15LDD286 pKa = 3.54PDD288 pKa = 3.14YY289 pKa = 10.58WYY291 pKa = 10.92LSGGGLVSVQEE302 pKa = 4.45MVNEE306 pKa = 3.95IQSGIEE312 pKa = 3.66

Molecular weight:
33.86 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A0B5ALD0|A0A0B5ALD0_9BACL tRNA dimethylallyltransferase OS=Jeotgalibacillus malaysiensis OX=1508404 GN=miaA PE=3 SV=1
MM1 pKa = 8.08AITQEE6 pKa = 4.12RR7 pKa = 11.84KK8 pKa = 10.14NEE10 pKa = 4.02IIQEE14 pKa = 4.13FRR16 pKa = 11.84THH18 pKa = 6.56EE19 pKa = 4.34GDD21 pKa = 3.3TGSVEE26 pKa = 4.13VQVAILTEE34 pKa = 4.94DD35 pKa = 3.29INNLNQHH42 pKa = 5.96LRR44 pKa = 11.84THH46 pKa = 6.54KK47 pKa = 10.02KK48 pKa = 9.14DD49 pKa = 3.03HH50 pKa = 6.33HH51 pKa = 6.06SRR53 pKa = 11.84RR54 pKa = 11.84GLFKK58 pKa = 10.02MVGRR62 pKa = 11.84RR63 pKa = 11.84RR64 pKa = 11.84NLLTYY69 pKa = 10.47LRR71 pKa = 11.84NNDD74 pKa = 2.95VARR77 pKa = 11.84YY78 pKa = 9.44RR79 pKa = 11.84EE80 pKa = 4.66LINKK84 pKa = 9.78LGLRR88 pKa = 11.84RR89 pKa = 3.7

Molecular weight:
10.63 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4426

0

4426

1165848

37

2235

263.4

29.55

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.338 ± 0.044

0.63 ± 0.013

5.517 ± 0.033

8.21 ± 0.051

4.432 ± 0.028

6.933 ± 0.04

2.16 ± 0.019

7.162 ± 0.036

6.526 ± 0.039

9.439 ± 0.039

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.965 ± 0.019

4.068 ± 0.03

3.522 ± 0.022

3.744 ± 0.027

4.171 ± 0.033

6.173 ± 0.032

5.486 ± 0.035

7.051 ± 0.03

1.027 ± 0.016

3.448 ± 0.024

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski