Methylobacterium tarhaniae

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Hyphomicrobiales; Methylobacteriaceae; Methylobacterium

Average proteome isoelectric point is 6.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 5746 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0J6V5T0|A0A0J6V5T0_9RHIZ Beta-ketoacyl synthase OS=Methylobacterium tarhaniae OX=1187852 GN=VQ03_23540 PE=3 SV=1
MM1 pKa = 7.13ATILGTNGNDD11 pKa = 3.04VLTGTTGDD19 pKa = 3.9DD20 pKa = 3.92VILGLLGNDD29 pKa = 4.31RR30 pKa = 11.84ISDD33 pKa = 3.46PGGFNRR39 pKa = 11.84IDD41 pKa = 3.75GQDD44 pKa = 3.5GADD47 pKa = 3.92VITGGANLDD56 pKa = 4.32YY57 pKa = 10.87IAGGPGNDD65 pKa = 3.36VIYY68 pKa = 10.63GGGGADD74 pKa = 3.55QLIGEE79 pKa = 5.02AGDD82 pKa = 3.81DD83 pKa = 4.54LIYY86 pKa = 10.86GQDD89 pKa = 3.55GDD91 pKa = 5.55DD92 pKa = 3.75YY93 pKa = 11.91AAGNPGNDD101 pKa = 3.74TIYY104 pKa = 11.07GGAGNDD110 pKa = 3.53FFVGEE115 pKa = 4.06QGNDD119 pKa = 3.18QVYY122 pKa = 10.66GEE124 pKa = 4.77AGNDD128 pKa = 3.61FVAGGEE134 pKa = 4.03DD135 pKa = 3.98DD136 pKa = 5.35DD137 pKa = 5.28LVSGGDD143 pKa = 3.64GDD145 pKa = 5.31DD146 pKa = 5.34LVDD149 pKa = 4.57GDD151 pKa = 5.14LGNDD155 pKa = 3.66TLLGDD160 pKa = 4.6AGNDD164 pKa = 3.46VLFGDD169 pKa = 4.34YY170 pKa = 11.29GNDD173 pKa = 3.29RR174 pKa = 11.84MNGGPGNDD182 pKa = 3.47RR183 pKa = 11.84LDD185 pKa = 3.91GAVGTDD191 pKa = 3.04TAVFDD196 pKa = 3.67TAFRR200 pKa = 11.84NLRR203 pKa = 11.84VTSSGSLVTFEE214 pKa = 4.39GATGIDD220 pKa = 3.42EE221 pKa = 4.54VKK223 pKa = 9.32NTEE226 pKa = 3.76VFEE229 pKa = 4.37FSDD232 pKa = 3.64RR233 pKa = 11.84TIVQADD239 pKa = 3.44GNAAVDD245 pKa = 3.61DD246 pKa = 4.81LYY248 pKa = 11.65YY249 pKa = 10.88LSRR252 pKa = 11.84NADD255 pKa = 3.38VLLAGLDD262 pKa = 3.73AEE264 pKa = 4.61AHH266 pKa = 6.03FGQYY270 pKa = 7.65GWRR273 pKa = 11.84EE274 pKa = 3.68GRR276 pKa = 11.84NPNAYY281 pKa = 9.96FDD283 pKa = 3.99TKK285 pKa = 10.9GYY287 pKa = 9.62LAAYY291 pKa = 9.62SDD293 pKa = 3.9VAAAGIDD300 pKa = 3.76PLQHH304 pKa = 5.55YY305 pKa = 8.23LQYY308 pKa = 10.55GWKK311 pKa = 9.81EE312 pKa = 3.86GRR314 pKa = 11.84DD315 pKa = 3.41PSANFDD321 pKa = 3.57TKK323 pKa = 11.13AYY325 pKa = 10.23LAANPDD331 pKa = 3.2VAAAGINPLEE341 pKa = 4.8HH342 pKa = 6.45FLQYY346 pKa = 11.23GSVEE350 pKa = 3.99GRR352 pKa = 11.84AVQPGDD358 pKa = 3.38GAFATATAPGVYY370 pKa = 8.56TT371 pKa = 4.26

Molecular weight: 38.28 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0J6SXX4|A0A0J6SXX4_9RHIZ Uncharacterized protein OS=Methylobacterium tarhaniae OX=1187852 GN=VQ03_16955 PE=4 SV=1
MM1 pKa = 7.35KK2 pKa = 9.43RR3 pKa = 11.84TYY5 pKa = 10.27QPSKK9 pKa = 9.73LVRR12 pKa = 11.84KK13 pKa = 9.15RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.42GFRR19 pKa = 11.84ARR21 pKa = 11.84MATVGGRR28 pKa = 11.84KK29 pKa = 9.31VIAARR34 pKa = 11.84RR35 pKa = 11.84ARR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 9.03RR41 pKa = 11.84LSAA44 pKa = 4.03

Molecular weight: 5.14 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

5746

0

5746

1734764

29

3241

301.9

32.35

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

14.485 ± 0.055

0.808 ± 0.01

5.471 ± 0.026

5.504 ± 0.03

3.296 ± 0.022

9.257 ± 0.038

1.951 ± 0.016

4.2 ± 0.027

2.325 ± 0.024

10.679 ± 0.041

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.05 ± 0.015

1.962 ± 0.02

5.946 ± 0.036

2.772 ± 0.019

8.282 ± 0.038

4.704 ± 0.023

5.271 ± 0.029

7.76 ± 0.031

1.259 ± 0.012

2.019 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski