Candidatus Synechococcus spongiarum LMB bulk10D

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Cyanobacteria/Melainabacteria group; Cyanobacteria; Synechococcales; Synechococcaceae; Synechococcus; Candidatus Synechococcus spongiarum

Average proteome isoelectric point is 6.89

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 532 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A6N4ASP3|A0A6N4ASP3_9SYNE Transcriptional repressor OS=Candidatus Synechococcus spongiarum LMB bulk10D OX=1449121 GN=BO98_00165 PE=3 SV=1
MM1 pKa = 7.57AVGIYY6 pKa = 9.88FATTTGKK13 pKa = 7.71TQDD16 pKa = 3.25VAEE19 pKa = 4.63RR20 pKa = 11.84LHH22 pKa = 6.83ALLSSANAPKK32 pKa = 10.59DD33 pKa = 3.69LADD36 pKa = 3.67MADD39 pKa = 3.09VSEE42 pKa = 4.19FTEE45 pKa = 4.96LDD47 pKa = 3.48GLICGVPTWNTGAEE61 pKa = 4.26TEE63 pKa = 4.27RR64 pKa = 11.84SGTAWDD70 pKa = 4.6GVLEE74 pKa = 5.3DD75 pKa = 4.93IAEE78 pKa = 4.36LDD80 pKa = 3.86LNGKK84 pKa = 8.98FVAVFGLGDD93 pKa = 3.59SGSYY97 pKa = 9.69TDD99 pKa = 4.21NFCDD103 pKa = 5.08AMEE106 pKa = 4.29EE107 pKa = 3.89LHH109 pKa = 7.04NFFKK113 pKa = 10.87QAGATMVGYY122 pKa = 9.12MDD124 pKa = 3.69KK125 pKa = 11.21SNYY128 pKa = 7.86TFEE131 pKa = 4.23EE132 pKa = 4.42SKK134 pKa = 11.06SVIGDD139 pKa = 4.01KK140 pKa = 10.9FCGLALDD147 pKa = 4.73EE148 pKa = 5.47DD149 pKa = 4.75SEE151 pKa = 5.92SDD153 pKa = 3.43MTDD156 pKa = 2.95DD157 pKa = 6.22RR158 pKa = 11.84LAKK161 pKa = 9.48WAEE164 pKa = 3.86QLKK167 pKa = 10.13QEE169 pKa = 4.6IPGLL173 pKa = 3.83

Molecular weight: 18.78 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A6N4ATU9|A0A6N4ATU9_9SYNE Rubredoxin OS=Candidatus Synechococcus spongiarum LMB bulk10D OX=1449121 GN=BO98_02760 PE=3 SV=1
MM1 pKa = 7.15ITTASRR7 pKa = 11.84QSALEE12 pKa = 3.79RR13 pKa = 11.84RR14 pKa = 11.84KK15 pKa = 10.38ALATAGKK22 pKa = 9.61AAASRR27 pKa = 11.84YY28 pKa = 9.97AKK30 pKa = 9.37TRR32 pKa = 11.84QRR34 pKa = 11.84NISDD38 pKa = 3.46EE39 pKa = 3.87QSPRR43 pKa = 11.84RR44 pKa = 11.84GHH46 pKa = 5.22VVTRR50 pKa = 11.84TVSVTPRR57 pKa = 11.84PRR59 pKa = 11.84PTVVFSPAAVAQPAPAGTPNLNTRR83 pKa = 11.84KK84 pKa = 9.65ISNPTRR90 pKa = 11.84DD91 pKa = 3.05LALARR96 pKa = 11.84RR97 pKa = 11.84QALSRR102 pKa = 11.84QGKK105 pKa = 10.08RR106 pKa = 11.84NDD108 pKa = 3.53PGNNRR113 pKa = 11.84IRR115 pKa = 11.84TEE117 pKa = 3.49VRR119 pKa = 11.84RR120 pKa = 11.84PSRR123 pKa = 11.84TTPQPAVPDD132 pKa = 3.88RR133 pKa = 11.84QDD135 pKa = 3.05TSHH138 pKa = 6.61TCDD141 pKa = 3.01CRR143 pKa = 11.84KK144 pKa = 10.16RR145 pKa = 11.84PGKK148 pKa = 8.41EE149 pKa = 3.43VRR151 pKa = 11.84KK152 pKa = 9.71PNLTSLSTGRR162 pKa = 11.84RR163 pKa = 11.84STAEE167 pKa = 3.86ARR169 pKa = 11.84LKK171 pKa = 9.48TPKK174 pKa = 10.43AKK176 pKa = 10.08NAKK179 pKa = 8.64NPSRR183 pKa = 11.84ALVLARR189 pKa = 11.84RR190 pKa = 11.84LAMSKK195 pKa = 10.04HH196 pKa = 5.51GKK198 pKa = 8.44SANAPTSASSAAVARR213 pKa = 11.84QGNPEE218 pKa = 3.82LSGRR222 pKa = 11.84EE223 pKa = 3.43LAQRR227 pKa = 11.84VRR229 pKa = 11.84EE230 pKa = 4.06LKK232 pKa = 10.61ARR234 pKa = 11.84SGSAGQCVKK243 pKa = 10.91GNNKK247 pKa = 9.46RR248 pKa = 11.84PCGPRR253 pKa = 11.84RR254 pKa = 11.84GMNRR258 pKa = 11.84PKK260 pKa = 11.21DD261 pKa = 3.51NDD263 pKa = 3.62QPVQAADD270 pKa = 3.47AHH272 pKa = 5.91WKK274 pKa = 10.05VGASTTPSGQTVTGTRR290 pKa = 11.84TDD292 pKa = 3.26RR293 pKa = 11.84SVKK296 pKa = 7.61TTGNEE301 pKa = 3.52ASTCRR306 pKa = 11.84TITGTEE312 pKa = 3.88YY313 pKa = 10.03MGADD317 pKa = 3.71VFQSYY322 pKa = 9.69CQSQAPSRR330 pKa = 11.84PRR332 pKa = 11.84KK333 pKa = 9.96VGATPTGYY341 pKa = 11.12GNTVTGTEE349 pKa = 3.87VGRR352 pKa = 11.84SVKK355 pKa = 9.31VTGDD359 pKa = 3.29EE360 pKa = 4.31PGTCKK365 pKa = 10.48SVTGTQYY372 pKa = 11.37LSGNHH377 pKa = 6.21GEE379 pKa = 4.43TWCGSPTQPQPAKK392 pKa = 10.25VGQTRR397 pKa = 11.84TQAEE401 pKa = 4.02QRR403 pKa = 11.84VSGVMVGRR411 pKa = 11.84SARR414 pKa = 11.84VTGDD418 pKa = 3.15EE419 pKa = 4.48PGSGRR424 pKa = 11.84QLTGSQYY431 pKa = 10.47MNPTPDD437 pKa = 3.4MGRR440 pKa = 11.84GPAPAKK446 pKa = 10.14VRR448 pKa = 11.84SFSTLRR454 pKa = 11.84GSAVTGNQVGRR465 pKa = 11.84STKK468 pKa = 9.42VTGDD472 pKa = 3.38EE473 pKa = 4.5PGTCKK478 pKa = 10.52NVTGDD483 pKa = 4.08DD484 pKa = 3.78YY485 pKa = 11.82VGRR488 pKa = 11.84QQYY491 pKa = 10.71DD492 pKa = 3.45AFCEE496 pKa = 4.03SRR498 pKa = 11.84PASEE502 pKa = 3.85AAKK505 pKa = 10.49VGLSATNRR513 pKa = 11.84NQIVSGTYY521 pKa = 8.41TGRR524 pKa = 11.84SPLVTGDD531 pKa = 3.48EE532 pKa = 4.45PGTCKK537 pKa = 10.48VVTGTPYY544 pKa = 10.97AGLEE548 pKa = 3.88QAGNFCSAPEE558 pKa = 3.7QATIVARR565 pKa = 11.84TPAGPGTPGQPMTGLQPGIGGVMTGAEE592 pKa = 4.04RR593 pKa = 11.84GACEE597 pKa = 4.3PVTGTPYY604 pKa = 10.67VGADD608 pKa = 3.45QFLSACDD615 pKa = 3.19QGGAGDD621 pKa = 5.07AVPADD626 pKa = 4.09LPQPLEE632 pKa = 4.08GAPWTRR638 pKa = 11.84FTVMSPFRR646 pKa = 11.84AAHH649 pKa = 5.7TEE651 pKa = 3.58HH652 pKa = 6.82TEE654 pKa = 3.87QKK656 pKa = 10.03RR657 pKa = 11.84RR658 pKa = 11.84LATGVTGTLYY668 pKa = 10.64EE669 pKa = 4.25RR670 pKa = 11.84GGTRR674 pKa = 11.84ITGPFGMAGGRR685 pKa = 11.84VTGTEE690 pKa = 3.89EE691 pKa = 3.94FRR693 pKa = 11.84FDD695 pKa = 3.58QRR697 pKa = 11.84GTNVSLPSRR706 pKa = 11.84NPLQPLASKK715 pKa = 11.07ADD717 pKa = 3.58AFAGATSPAPVAPPAPSRR735 pKa = 11.84VTGEE739 pKa = 3.9GMVQGVRR746 pKa = 11.84ISGDD750 pKa = 2.81DD751 pKa = 2.98WSRR754 pKa = 11.84NEE756 pKa = 4.14RR757 pKa = 11.84VTGTEE762 pKa = 4.09GPSARR767 pKa = 11.84RR768 pKa = 11.84RR769 pKa = 11.84NPSRR773 pKa = 11.84PGPMSAMHH781 pKa = 7.26AFQLKK786 pKa = 9.78RR787 pKa = 11.84NAEE790 pKa = 4.3TEE792 pKa = 4.41VPLSPITGSSGNTDD806 pKa = 2.66KK807 pKa = 11.5GAVVTFSGGARR818 pKa = 11.84GG819 pKa = 3.36

Molecular weight: 86.05 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

532

0

532

149200

21

1132

280.5

30.75

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.254 ± 0.126

1.346 ± 0.044

5.22 ± 0.079

5.422 ± 0.095

3.198 ± 0.067

8.252 ± 0.108

2.523 ± 0.065

4.041 ± 0.087

2.902 ± 0.081

11.642 ± 0.154

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.121 ± 0.041

2.757 ± 0.062

5.611 ± 0.084

4.703 ± 0.077

7.584 ± 0.099

5.832 ± 0.079

5.41 ± 0.098

7.582 ± 0.092

1.518 ± 0.049

2.082 ± 0.051
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski