Flavobacterium psychrotolerans

Taxonomy: cellular organisms; Bacteria; FCB group; Bacteroidetes/Chlorobi group; Bacteroidetes; Flavobacteriia; Flavobacteriales; Flavobacteriaceae; Flavobacterium

Average proteome isoelectric point is 6.8

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2753 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    

Protein with the lowest isoelectric point:
>tr|A0A2U1JMW5|A0A2U1JMW5_9FLAO Urease accessory protein UreE OS=Flavobacterium psychrotolerans OX=2169410 GN=ureE PE=3 SV=1
MM1 pKa = 7.51KK2 pKa = 10.42KK3 pKa = 8.47ITLLFMLFIGFSAFAQFPQDD23 pKa = 3.54FEE25 pKa = 4.86GTFPPTGWAVYY36 pKa = 10.08RR37 pKa = 11.84GANDD41 pKa = 4.43LGIEE45 pKa = 4.22SDD47 pKa = 3.4WTATTVGVATGLQAAYY63 pKa = 10.5VDD65 pKa = 4.39YY66 pKa = 10.8EE67 pKa = 4.13AVTAGEE73 pKa = 4.26LAEE76 pKa = 4.79DD77 pKa = 3.9WLVSPAYY84 pKa = 9.28TVTAPNTLMAFYY96 pKa = 10.11QGQDD100 pKa = 3.12DD101 pKa = 4.03PTDD104 pKa = 3.52YY105 pKa = 11.36GSTYY109 pKa = 9.27TIRR112 pKa = 11.84VSTTSQIDD120 pKa = 3.4INSFTIVDD128 pKa = 4.01TQTEE132 pKa = 4.34SDD134 pKa = 3.75FTIEE138 pKa = 3.9MTTRR142 pKa = 11.84TVDD145 pKa = 3.51LSAYY149 pKa = 9.63AGQTIYY155 pKa = 10.61IAFVLEE161 pKa = 4.15NNEE164 pKa = 3.58GDD166 pKa = 3.06AWAIDD171 pKa = 4.43GIDD174 pKa = 4.05FISNTVIAPGCVSNPSPADD193 pKa = 3.12AALEE197 pKa = 4.21VPIGATTFSWTAPTTGGPVTLYY219 pKa = 10.64NIYY222 pKa = 10.73SGATSDD228 pKa = 4.45AVTDD232 pKa = 5.27FITSTTTPSVDD243 pKa = 3.54LSLADD248 pKa = 4.71FSTTFYY254 pKa = 10.18WKK256 pKa = 10.05VVAVNLVGSATDD268 pKa = 3.46CAVWSFTTQSPPGYY282 pKa = 9.57CLAASGGQYY291 pKa = 10.05PADD294 pKa = 3.97AYY296 pKa = 9.99TPEE299 pKa = 4.52ILDD302 pKa = 3.76GVTEE306 pKa = 4.41NIIVSDD312 pKa = 4.19GFAGEE317 pKa = 4.04YY318 pKa = 10.45SVVNVLSGQTYY329 pKa = 7.8TFTSGTTDD337 pKa = 4.9FITIGNEE344 pKa = 3.76AGDD347 pKa = 3.67TSLIAGASPLTWVSDD362 pKa = 3.13ISGVIRR368 pKa = 11.84FYY370 pKa = 11.61SHH372 pKa = 7.32VDD374 pKa = 3.48DD375 pKa = 4.26QCGEE379 pKa = 4.12EE380 pKa = 4.32NIEE383 pKa = 4.07RR384 pKa = 11.84PRR386 pKa = 11.84TVISDD391 pKa = 3.86PLLATDD397 pKa = 4.73TFNSASFHH405 pKa = 5.7TFPNPVKK412 pKa = 10.59DD413 pKa = 3.88ILNISYY419 pKa = 10.42DD420 pKa = 3.76KK421 pKa = 11.01NISNVAVFNLLGQEE435 pKa = 4.16VMTKK439 pKa = 8.87SVKK442 pKa = 9.96QNQSQVDD449 pKa = 3.91MSHH452 pKa = 6.92LSNGTYY458 pKa = 8.32MVKK461 pKa = 8.8VTSDD465 pKa = 3.46NQVKK469 pKa = 8.88TIKK472 pKa = 10.31VIKK475 pKa = 10.09GG476 pKa = 3.21

Molecular weight:
51.05 kDa
Isoelectric point according different methods:






Protein with the highest isoelectric point:
>tr|A0A2U1JFJ3|A0A2U1JFJ3_9FLAO IS91 family transposase OS=Flavobacterium psychrotolerans OX=2169410 GN=DB895_14025 PE=4 SV=1
RR1 pKa = 7.43GAPGVDD7 pKa = 2.48RR8 pKa = 11.84LTVEE12 pKa = 4.37EE13 pKa = 4.4LKK15 pKa = 10.93DD16 pKa = 3.23WLLVHH21 pKa = 6.55WPSVKK26 pKa = 10.32AALLEE31 pKa = 4.08DD32 pKa = 4.47RR33 pKa = 11.84YY34 pKa = 10.91LPRR37 pKa = 11.84AVRR40 pKa = 11.84RR41 pKa = 11.84VDD43 pKa = 3.0IPKK46 pKa = 9.93PNGGVRR52 pKa = 11.84TLGVPTVVDD61 pKa = 3.65RR62 pKa = 11.84LIQQALHH69 pKa = 5.99QVLQPIFEE77 pKa = 4.52PTFSDD82 pKa = 3.39GSYY85 pKa = 10.61GFRR88 pKa = 11.84PQRR91 pKa = 11.84SALDD95 pKa = 3.4AVVKK99 pKa = 10.48AKK101 pKa = 10.59EE102 pKa = 3.82YY103 pKa = 9.61VAEE106 pKa = 4.68GKK108 pKa = 9.58HH109 pKa = 4.47WVVDD113 pKa = 3.15IDD115 pKa = 3.99LEE117 pKa = 4.57KK118 pKa = 11.04FFDD121 pKa = 3.98RR122 pKa = 11.84VNHH125 pKa = 6.24DD126 pKa = 3.15VLMARR131 pKa = 11.84LAYY134 pKa = 9.58RR135 pKa = 11.84VRR137 pKa = 11.84DD138 pKa = 3.62GRR140 pKa = 11.84VLKK143 pKa = 10.51LIRR146 pKa = 11.84RR147 pKa = 11.84FLEE150 pKa = 4.05AGMMANGVIQPRR162 pKa = 11.84SEE164 pKa = 3.88GTPQGGPLSPLLSNILLTDD183 pKa = 4.08LDD185 pKa = 4.16RR186 pKa = 11.84EE187 pKa = 4.33LEE189 pKa = 3.97RR190 pKa = 11.84RR191 pKa = 11.84QLKK194 pKa = 8.83FCRR197 pKa = 11.84YY198 pKa = 10.23ADD200 pKa = 3.8DD201 pKa = 4.04CNIYY205 pKa = 10.13IGSEE209 pKa = 4.15GAGQRR214 pKa = 11.84AMANIRR220 pKa = 11.84AFLEE224 pKa = 4.02GHH226 pKa = 6.27LKK228 pKa = 10.61LRR230 pKa = 11.84INEE233 pKa = 4.05QKK235 pKa = 10.77SAVARR240 pKa = 11.84PWKK243 pKa = 10.37RR244 pKa = 11.84KK245 pKa = 8.5FLGYY249 pKa = 10.52AITIYY254 pKa = 10.3RR255 pKa = 11.84QEE257 pKa = 4.05TRR259 pKa = 11.84VRR261 pKa = 11.84PAPEE265 pKa = 3.66SLRR268 pKa = 11.84RR269 pKa = 11.84LMDD272 pKa = 3.49RR273 pKa = 11.84VRR275 pKa = 11.84EE276 pKa = 3.97LLRR279 pKa = 11.84KK280 pKa = 9.72GRR282 pKa = 11.84GRR284 pKa = 11.84SLSHH288 pKa = 6.64TIEE291 pKa = 3.9MLNPVLRR298 pKa = 11.84GWANYY303 pKa = 8.69FRR305 pKa = 11.84LTANMRR311 pKa = 11.84MLEE314 pKa = 3.99EE315 pKa = 4.4LDD317 pKa = 2.56WWLRR321 pKa = 11.84RR322 pKa = 11.84KK323 pKa = 9.23LRR325 pKa = 11.84CLLWRR330 pKa = 11.84QWKK333 pKa = 7.62TPKK336 pKa = 9.11TRR338 pKa = 11.84EE339 pKa = 3.55RR340 pKa = 11.84RR341 pKa = 11.84LILLGLRR348 pKa = 11.84PEE350 pKa = 4.41RR351 pKa = 11.84AWKK354 pKa = 10.52SSVNGRR360 pKa = 11.84GPWWNSASKK369 pKa = 10.6HH370 pKa = 4.88MNQAVPTLYY379 pKa = 10.88

Molecular weight:
44.12 kDa
Isoelectric point according different methods:






Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2753

0

2753

953119

34

3340

346.2

38.88

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.433 ± 0.061

0.824 ± 0.016

5.124 ± 0.036

6.068 ± 0.071

5.385 ± 0.044

6.521 ± 0.062

1.689 ± 0.025

8.414 ± 0.044

7.886 ± 0.072

9.129 ± 0.06

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.223 ± 0.025

6.398 ± 0.049

3.4 ± 0.027

3.346 ± 0.025

3.061 ± 0.03

6.679 ± 0.052

6.251 ± 0.103

6.254 ± 0.037

1.003 ± 0.018

3.911 ± 0.031

Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski