Pectobacterium phage PP81

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Studiervirinae; Pektosvirus; Pectobacterium virus PP81

Average proteome isoelectric point is 6.86

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 53 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1L7DS89|A0A1L7DS89_9CAUD Holin OS=Pectobacterium phage PP81 OX=1927014 GN=PP81_gp49 PE=3 SV=1
MM1 pKa = 7.7AEE3 pKa = 4.14SNADD7 pKa = 3.26VYY9 pKa = 11.66ASFGVNPAVVTSGSVSEE26 pKa = 5.25HH27 pKa = 4.95EE28 pKa = 4.21QAMLEE33 pKa = 4.18LDD35 pKa = 3.49VSARR39 pKa = 11.84DD40 pKa = 3.39GDD42 pKa = 4.15DD43 pKa = 4.77AITLAEE49 pKa = 4.22MPDD52 pKa = 3.79VQSDD56 pKa = 3.7PNDD59 pKa = 3.84PYY61 pKa = 11.86GNPDD65 pKa = 3.66KK66 pKa = 10.92FADD69 pKa = 4.15PNDD72 pKa = 3.79DD73 pKa = 2.75SRR75 pKa = 11.84MQIRR79 pKa = 11.84INADD83 pKa = 3.23GEE85 pKa = 4.75TVEE88 pKa = 4.86MDD90 pKa = 3.98ADD92 pKa = 3.89GQPIEE97 pKa = 4.89GDD99 pKa = 3.42GDD101 pKa = 3.77DD102 pKa = 4.85TEE104 pKa = 4.46FTPLGEE110 pKa = 4.21PSEE113 pKa = 4.2EE114 pKa = 3.8LSASVEE120 pKa = 3.85LLGQHH125 pKa = 5.62EE126 pKa = 4.86AGFQEE131 pKa = 4.6MVNQAGEE138 pKa = 4.03RR139 pKa = 11.84GLAAEE144 pKa = 4.81SIQRR148 pKa = 11.84IQLEE152 pKa = 4.19YY153 pKa = 9.98QADD156 pKa = 4.75GISEE160 pKa = 3.85QSYY163 pKa = 10.72EE164 pKa = 3.96EE165 pKa = 4.09LAAAGYY171 pKa = 10.38SKK173 pKa = 11.3AFVDD177 pKa = 5.06SYY179 pKa = 11.67IKK181 pKa = 10.65GQEE184 pKa = 4.03ALVDD188 pKa = 4.25GYY190 pKa = 11.11VRR192 pKa = 11.84QVQQMAGGEE201 pKa = 4.18EE202 pKa = 4.49KK203 pKa = 9.79FTNIMSHH210 pKa = 6.84LEE212 pKa = 4.14STNKK216 pKa = 9.32EE217 pKa = 4.03AAEE220 pKa = 4.02SLYY223 pKa = 11.14AALAARR229 pKa = 11.84DD230 pKa = 3.58LATVKK235 pKa = 10.9GILNLAGEE243 pKa = 4.41SRR245 pKa = 11.84AKK247 pKa = 10.72KK248 pKa = 9.73FGKK251 pKa = 8.95PAEE254 pKa = 4.05RR255 pKa = 11.84SVTQRR260 pKa = 11.84ATPSKK265 pKa = 9.59AAVVKK270 pKa = 11.01AEE272 pKa = 4.17GFGSSEE278 pKa = 4.09EE279 pKa = 4.11MVKK282 pKa = 10.77AMSDD286 pKa = 2.97PRR288 pKa = 11.84YY289 pKa = 8.98RR290 pKa = 11.84TDD292 pKa = 2.8SKK294 pKa = 10.77FRR296 pKa = 11.84QEE298 pKa = 4.24VEE300 pKa = 4.05QKK302 pKa = 10.28VAASSFGWW310 pKa = 3.36

Molecular weight: 33.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7S8WIF3|A0A7S8WIF3_9CAUD Terminase small subunit OS=Pectobacterium phage PP81 OX=1927014 GN=PP81_gp50 PE=4 SV=1
MM1 pKa = 7.48LKK3 pKa = 10.66APIKK7 pKa = 10.49ASTTIRR13 pKa = 11.84LSDD16 pKa = 4.27TVDD19 pKa = 2.55QWSRR23 pKa = 11.84RR24 pKa = 11.84IHH26 pKa = 5.76VNVRR30 pKa = 11.84NGKK33 pKa = 6.82PTLVYY38 pKa = 9.87RR39 pKa = 11.84WRR41 pKa = 11.84DD42 pKa = 3.35SKK44 pKa = 11.39SPKK47 pKa = 9.74SHH49 pKa = 5.11TQRR52 pKa = 11.84VTLSDD57 pKa = 3.66VQVGRR62 pKa = 11.84LIAALSIATGVAADD76 pKa = 3.92NNEE79 pKa = 3.63SRR81 pKa = 11.84LLVVANGMDD90 pKa = 3.43AQQRR94 pKa = 11.84EE95 pKa = 4.28AGII98 pKa = 4.0

Molecular weight: 10.81 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

53

0

53

12669

25

1338

239.0

26.59

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.338 ± 0.54

0.924 ± 0.16

6.204 ± 0.236

6.401 ± 0.264

3.923 ± 0.215

7.585 ± 0.318

1.863 ± 0.197

5.004 ± 0.272

6.457 ± 0.337

8.256 ± 0.29

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.771 ± 0.177

4.712 ± 0.266

3.584 ± 0.173

3.868 ± 0.311

5.367 ± 0.233

6.086 ± 0.312

5.636 ± 0.264

7.072 ± 0.246

1.405 ± 0.164

3.544 ± 0.2
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski